Rapid detection of tulathromycin in pure milk and honey with an immunochromatographic test strip

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A rapid, specific and sensitive method based an immunochromatographic strip test has been developed for the determination of sodium nifurstyrenate (NFS) residues in fish. Sample preparation procedure includes ultrasonic bath extraction with acetonitrile, sample centrifugation at 8000 rpm for 10 min, filtration through the 0.22 µm membrane filter, and drying over nitrogen (60°C) and res-suspension. The antigen (NFS-ovalbumin) and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test line and control line, respectively. Under optimized conditions, the NFS cutoff limits for test strips was determined as 5 ng/mL in 0.01 M phosphate buffered saline and 10 ng/mL in fish. Results were obtained within 5 min. Our data suggests this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening for NFS in fish.

Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic strip test for rapid detection of sodium nifurstyrenate in fish

Song

Suryoprabowo

Liu

et al. 2019

Self Cite

“…Surfactants are essential for the efficient running of test strips due to incompatibility issues between the NC membrane and detection solution (Kong et al, 2019;Liu et al, 2017;. In this study, GNP-labelled mAb and sample solution were mixed with a basic buffer (0.02 M Tris-HCl supplemented with 5% sucrose, 0.1% PEG, 0.1% Tween-20, 5% trehalose and 0.2% BSA) containing different surfactants, including polyvinylpyrrolidone (PVP), PEG and BSA.…”

Section: Optimization Of the Icsmentioning

confidence: 99%

Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay

Jin

Liu

et al. 2020

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Citreoviridin (CTV) is a toxic secondary metabolite that can be found in mouldy cereals. In this study, a sensitive and ethanol-tolerant anti-CTV monoclonal antibody (6B2) was produced with a 50% inhibition concentration of 4.1 ng/mL and then labelled using gold nanoparticle (GNP). Based on competitive inhibition of the binding of CTV-COOH-BSA conjugate sprayed onto a membrane to limited GNP-labelled antibody by free CTV in the samples, an easy-to-operate immunochromatographic strip (ICS) was developed for the fast screening of CTV in rice. The results are seen as a colour change within 5-10 min. We used relatively safe ethanol instead of highly toxic methanol or chloroform as the extraction solution and found a visible detection limit of 100 μg/ kg and cut-off value of 1000 μg/kg. The developed ICS assay worked well and represented a reliable, safe, fast and a highthroughput method for the detection of CTV residues in rice samples.

“…Female BALB/c mice (6-8 weeks of age) were immunized with complete antigen (BPS-KLH). Tail vein blood sampling was carried out after the third immunization and serum was analyzed using an ic-ELISA (Liu, Xu, Suryoprabowo, Song, & Kuang, 2018). The mouse with the highest inhibitory rate and titre was selected for cell fusion.…”

Section: Production Of the Bps Monoclonal Antibody (Mab)mentioning

confidence: 99%

IC-ELISA and immunochromatographic strip assay based monoclonal antibody for the rapid detection of bisphenol S

Lü

Luo

et al. 2019

Self Cite

A highly sensitive and specific monoclonal antibody against bisphenol S (BPS) was prepared. The derived BPS was coupled to keyhole limpet hemocyanin as the immunogen and ovalbumin as the coating antigen. Based on monoclonal antibodies, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established, and the conditions of action were optimized. The half maximal inhibitory concentration (IC 50 ) of BPS was 0.228 ng/mL and the linear range was 0.064-0.810 ng/mL. In the recovery test for the milk samples, the recovery rates were in the range of 89%-95% and coefficient of variation ranged from 1.0% to 6.0% respectively. In addition, the cut-off value of the immunochromatographic strip detection method in milk samples was 5 ng/mL. Therefore, both methods are suitable for use in milk samples. Furthermore, this immunochromatographic strip detection method is suitable for on-site testing and screening of very large samples.