IC-ELISA and immunochromatographic strip assay based monoclonal antibody for the rapid detection of bisphenol S

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In this study, we produced a specific monoclonal antibody (mAb) against triadimefon (TDF) and triadimenol (TDN). The half-maximal inhibitory concentration for TDF and TDN was 5.25 and 9.98 ng/ mL, respectively, with a linear detection range (IC 20-IC 80) of 1.09-25.35 and 2.29-43.61 ng/mL, respectively. Subsequently, we developed a colloidal gold immunochromatography test strip for the analysis of TDF and TDN in tomato. The results obtained from the colloidal gold immunochromatography test strip were in accordance with those obtained from the indirect competitive enzyme-linked immunosorbent assay. The colloidal gold immunochromatography test strip is efficient and suitable for onsite detection of TDF and TDN.

“…2.7.3. Sensitivity and specificity of mAb MAb sensitivity and specificity against TDF and TDN were determined by ic-ELISA (Lin et al, 2019;Z. Wang et al, 2019).…”

Section: Characterization Of Ic-elisamentioning

confidence: 99%

A colloidal gold immunochromatography test strip based on a monoclonal antibody for the rapid detection of triadimefon and triadimenol in foods

Lü

Song

et al. 2020

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“…GNPs possesses useful optical properties and can serve as a visible detection probes (Lin et al, 2019). GNPs with a diameter of about 15 nm appear red wine in colour and were supplied by our laboratory.…”

Section: Synthesis Of Gnp-labelled Mabmentioning

confidence: 99%

Fast determination of citreoviridin residues in rice using a monoclonal antibody-based immunochromatographic strip assay

Jin

Liu

et al. 2020

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Citreoviridin (CTV) is a toxic secondary metabolite that can be found in mouldy cereals. In this study, a sensitive and ethanol-tolerant anti-CTV monoclonal antibody (6B2) was produced with a 50% inhibition concentration of 4.1 ng/mL and then labelled using gold nanoparticle (GNP). Based on competitive inhibition of the binding of CTV-COOH-BSA conjugate sprayed onto a membrane to limited GNP-labelled antibody by free CTV in the samples, an easy-to-operate immunochromatographic strip (ICS) was developed for the fast screening of CTV in rice. The results are seen as a colour change within 5-10 min. We used relatively safe ethanol instead of highly toxic methanol or chloroform as the extraction solution and found a visible detection limit of 100 μg/ kg and cut-off value of 1000 μg/kg. The developed ICS assay worked well and represented a reliable, safe, fast and a highthroughput method for the detection of CTV residues in rice samples.

“…The capture and control reagents were then sprayed onto a glass fibre membrane to prepare a conjugate pad and dried at 37°C for 2 h. An NC film coated with the capture reagent was attached to the centre of the clinker back sheet (PVC), and the conjugate pad, sample pad and absorbent pads were laminated and adhered to the back sheet. Finally, the plate was cut into 2.8 mm wide strips (Lin et al, 2019).…”

Section: Preparation Of Nc Capture Membranementioning

confidence: 99%

Development of an immunocolloidal strip for rapid detection of picoxystrobin

Song

Chen

Liu

et al. 2020

Self Cite

In this study, we developed a monoclonal antibody against picoxystrobin (PIC). After cell fusion, four cell lines (3C2, 4D1, 4G4 and 5D4) capable of producing monoclonal antibodies against oxystrobin were obtained. The monoclonal antibody produced by the cell line 3C2 was the most sensitive to PIC, and its IC 50 value was 0.1 ng/ml. The monoclonal antibody was then used to prepare immunochromatographic strips, and its cut-off values in both phosphate-buffered saline solution and cucumber samples were 50 and 100 ng/ml, respectively. It was concluded that the ICS can sensitively and rapidly detect PIC in cucumber samples.