Rapid detection of Trypanosoma cruzi by colorimetric loop-mediated isothermal amplification (LAMP): A potential novel tool for the detection of congenital Chagas infection

Rivero, Rocío; Bisio, Margarita; Velázquez, Elsa; Esteva, M; Scollo, Karenina; González, Nicolás; Altcheh, Jaime; Ruiz, Andrés M.

doi:10.1016/j.diagmicrobio.2017.06.012

Cited by 38 publications

(26 citation statements)

References 14 publications

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“…Therefore, this prototype kit detects T.cruzi DNA at concentrations 1 x 10 −2 fg/µL using purified DNA and 1x10 −2 parasite equivalents/mL ("parasite equivalent" means the equivalent genomic content of one parasite cell) in extracts from spiked EDTA blood samples. The analytical sensitivity of this LAMP assay was superior to other studies using a LAMP procedure based on the 18S rDNA gene [107,109].…”

Section: Lamp In Diagnosis Of American Try-panosomiasis (T Cruzi)mentioning

confidence: 72%

Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

Dea-Ayuela

Galiana‐Roselló

Lalatsa

et al. 2018

CTMC

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One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector-borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of resistance. Molecular assays based on loopmediated isothermal amplification (LAMP) techniques are a good alternative to light microscopy and antigen-based rapid diagnostic tests in developing countries, since they allow for a large amount of genetic material generated from a few copies of DNA, and use primers that lead to high sensitivity and specificity, while the amplification process can be performed in isothermal conditions without the need of sophisticated equipment to interpret the results. In this review, the main advances in the development of LAMP assays for the diagnosis of malaria, leishmaniasis and Chagas' disease are discussed as well as the feasibility of their implementation in developing countries and use as point-of-care diagnostic tests.

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Section: Lamp In Diagnosis Of American Try-panosomiasis (T Cruzi)mentioning

confidence: 72%

Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

Dea-Ayuela

Galiana‐Roselló

Lalatsa

et al. 2018

CTMC

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“… 44 Other groups have developed similar LAMP kits to detect T. cruzi DNA. 45 Another platform that meets the criteria for implementation of molecular diagnosis at such sites is recombinase polymerase amplification (RPA), which also enables sensitive, specific and rapid amplification of DNA under isothermal conditions. The RPA reaction runs optimally at 37°C–42°C which may present an advantage in terms of energy costs saving.…”

Section: Implementation Of Molecular Tools To Test For Congenital Chamentioning

confidence: 99%

The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America

et al. 2018

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It is estimated that between 8000 and 15 000 Trypanosoma cruzi infected babies are born every year to infected mothers in Chagas disease endemic countries. Currently, poor access to and performance of the current diagnostic algorithm, based on microscopy at birth and serology at 8–12 months after delivery, is one of the barriers to congenital Chagas disease (CCD) control. Detection of parasite DNA using molecular diagnostic tools could be an alternative or complement to current diagnostic methods, but its implementation in endemic regions remains limited. Prompt diagnosis and treatment of CCD cases would have a positive clinical and epidemiological impact. In this paper, we analysed the burden of CCD in Latin America, and the potential use of molecular tests to improve access to early diagnosis and treatment of T. cruzi infected newborns.

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“…However, multiple infant specimens are required to maximize sensitivity [18]. A number of PCR assays are now available to detect congenital T. cruzi infections, including quantitative PCR [40] and a recent loop-mediated isothermal amplification (LAMP) protocol [51]. Molecular techniques require considerable equipment, expertise and rigorous quality control, which are difficult to implement and sustain in resource-limited settings.…”

Section: Congenital Chagas Disease Screening and Diagnosismentioning

confidence: 99%

Congenital Chagas disease: current diagnostics, limitations and future perspectives

Messenger

Bern

2018

Current Opinion in Infectious Diseases

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Congenital Chagas disease remains an important contributor to the global disease burden because of T. cruzi. PCR and related molecular techniques represent the most sensitive diagnostic modalities for early detection but require further optimization for resource-limited settings. Several novel diagnostic tests show promise for the future but further validation and adaptation to field settings are needed.

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Rapid detection of Trypanosoma cruzi by colorimetric loop-mediated isothermal amplification (LAMP): A potential novel tool for the detection of congenital Chagas infection

Cited by 38 publications

References 14 publications

Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

The burden of congenital Chagas disease and implementation of molecular diagnostic tools in Latin America

Congenital Chagas disease: current diagnostics, limitations and future perspectives

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