Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

Dea-Ayuela, M. Auxiliadora; Galiana‐Roselló, Cristina; Lalatsa, Aikaterini; Serrano, Dolores R.

doi:10.2174/1568026618666181025095735

Cited by 9 publications

(4 citation statements)

References 86 publications

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“…Nevertheless, sometimes the visualisation of magnesium pyrophosphate precipitate is a little bit difficult, especially when the DNA concentration is relatively low. A sample is considered to be positive or negative if an obvious increase in the turbidity is observed by the naked eye compared to the negative control, in addition, a LAMP test/result is considered valid if the turbidity is present in the positive control but completely absent in the negative control [64]. The result also can be interpreted by the use of ultraviolet (UV) fluorescent in the presence of an indicator such as Calcein.…”

Section: Loop-mediated Isothermal Amplificationmentioning

confidence: 99%

Advanced Techniques and Unusual Samples for Malaria Diagnosis

Muhammad,

Pukuma Sale,

Mahmoud Mohammed

2024

Infectious Diseases

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Successful malaria control, treatment, and prevention depends on successful diagnosis using appropriate equipment with high sensitivity and specificity. In most tropical countries where the disease is endemic, malaria diagnosis is still based on the conventional techniques (Microscopy and RDT) which have so many shortcomings, hence the need to switch to the most advanced diagnostic technique for better results. In this review, several serological and molecular malaria diagnostic techniques like Polymerase Chain Reaction (PCR), Flow cytometry, Loop-mediated Isothermal Amplification (LAMP), Indirect Immunofluorescence, Enzyme-Linked Immunosorbent Assay (ELISA), Radioimmunoassay (RIA), Quantitative Buffy Coat (QBC) and Laser Desorption Mass Spectrometry (LDMS) were systematically discussed in simple and direct language for easier understanding of the principle involved in each case scenario. In addition, some unusual samples for malaria diagnosis like Urine and saliva were also discussed.

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Section: Loop-mediated Isothermal Amplificationmentioning

confidence: 99%

Advanced Techniques and Unusual Samples for Malaria Diagnosis

Muhammad,

Pukuma Sale,

Mahmoud Mohammed

2024

Infectious Diseases

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“…Importantly, it does not have strict requirements in terms of infrastructure and resources, such as a reliable power supply or a temperature-controlled environment. This makes the implementation and scalability of decentralized LAMP diagnostics possible in lowresource settings (Notomi et al, 2000;Dea-Ayuela et al, 2018).…”

Section: Other Potential Uses Of Molecular Diagnostic Techniques In C...mentioning

confidence: 99%

Clinical use of molecular methods for Trypanosoma cruzi infection in endemic and non-endemic countries: Benefits, limitations and challenges

Pinazo,

Forsyth,

Lopez-Albizu

et al. 2023

Front. Parasitol.

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Trypanosoma cruzi infection is diagnosed by parasitological, molecular, and serological tests. Molecular methods based on DNA amplification provide a more sensitive alternative to classical parasitological techniques for detecting evidence of T. cruzi parasitemia, and are the preferred tests for congenital and oral transmission cases and parasite reactivation in chronically infected immunosuppressed individuals. In newborns at risk of vertical transmission, simplified diagnostic algorithms that provide timely results can reduce the high follow-up losses observed with current algorithms. Molecular methods have also proved useful for monitoring T. cruzi infection in solid organ transplantation recipients, regardless of host immune status, allowing parasite detection even before symptom manifestation. Furthermore, in the absence of other biomarkers and a practical test of cure, and given the limitations of serological methods, recent clinical guidelines have included polymerase chain reaction (PCR) to detect therapeutic failure after antiparasitic treatment in chronically infected adults. Increasing evidence supports the use of molecular tests in a clinical context, given the improved sensitivity and specificity of current assays – characteristics which largely depend on epidemiological factors and genetic and antigenic variability among T. cruzi strains. Further development and registration of commercial PCR kits will improve the use of molecular tests. We discuss the attributes of PCR and other molecular tests for clinical management in people with T. cruzi infection.

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“…The great burden of viral gastroenteritis on health care due to related illness and hospitalization highlights the need for fast, sensitive, and reliable diagnostic assays to guide infection control measures. The loop-mediated isothermal amplification (LAMP) assay has been successfully implemented for the detection of a wide range of pathogens of infectious diseases, including SARS-CoV-2 ( 10 ), Zika virus ( 11 , 12 ), enteroviruses ( 13 ), Dengue virus ( 14 ), tuberculosis ( 15 , 16 ), Ebola virus ( 17 ), influenza virus ( 18 ), Helicobacter pylori ( 19 ), Plasmodium falciparum ( 20 ), Leishmania ( 20 ), Trypanosoma ( 20 ), Treponema pallidum ( 21 ), and Haemophilus ducreyi ( 21 ). LAMP is a highly specific and sensitive reaction of DNA amplification, as LAMP significantly reduces the amplification time from 2 to 3 h to 15 to 30 min compared to that of PCR.…”

Section: Introductionmentioning

confidence: 99%