Rapid detection of thioredoxin reductase with a fluorescent probe via a Tag-Sec method

Abstract

A Tag-Sec fluorescent probe was designed that could detect both thioredoxin reductase and selenocysteine with high sensitivity, selectivity, broad pH tolerance and good water solubility.

A Fluorescent Probe to Detect Quick Disulfide Reductase Activity in Bacteria

“…In the last two decades, there has been a growing trend towards using fluorescent probes to determine the reactive sulfur species in vivo [ 21 , 22 ]. In recent years, studies have provided important information on the use of fluorescent probes to detect LMW thiols e.g., cysteine [ 23 ], as well as antioxidant enzymes, including mammalian TrxR [ 24 ] and Trx [ 25 , 26 ], whereas, there is a very limited understanding of cellular redox homeostasis in bacteria [ 27 ]. Besides the differences in the cell wall/cell membrane between bacterial and mammalian cells, there are great differences in the composition and function of thiol-dependent systems between two types of organisms.…”

Recent progress in the development of small-molecule fluorescent probes for detection and imaging of selenocysteine and application in thyroid disease diagnosis

“…This blocks the internal twisting of the nitrogen atom which otherwise results in quenching of the fluorescence of coumarin due to charge transfer from coumarin to 2,4-DNB. 47–49 The fluorescent probes L 2 , L 3 , and L 4 have also been reported to detect thiophenol. Motivated by the excellent selectivity and sensitivity of thiophenol probes, these were used for SeCys detection.…”

A fluorescent probe for specifically measuring the overall thioredoxin and glutaredoxin reducing activity in bacterial cells

“…11 So far, cellular small thiol molecules and single antioxidant enzymes have been well characterized with various probes, especially in mammalian cells. 12–18 However, there are few studies to clarify the exact intracellular contribution of various Trxs and Grxs in maintaining the cellular redox balance due to the lack of an effective approach to detect the overall disulfide-reducing activities of both systems. 11,19…”