Recent progress in the development of small-molecule fluorescent probes for detection and imaging of selenocysteine and application in thyroid disease diagnosis

Abstract

Selenocysteine (SeCys) is the 21st genetically encoded amino acid present in proteins and is involved in various biological functions.

Highly selective fluorescence detection of l-selenium-methylselenocysteine in selenium-enriched Cardamine violifolia

“…5 Fluorescence assay for SeCys has a lot of reports. [6][7][8][9] For example, Chen et al designed and synthesized a ratiometric near-infrared uorescent probe to analyze the uctuations and roles of SeCys in cells and in a mouse thyroid disease model. 10 Methyl selenocysteine (SeMC) has a similar molecular structure compared with SeCys and the molecular formula differs by only one -CH 2 .…”

A terbium(iii) complex-based time-resolved luminescent probe for selenocysteine as an inhibitor of selenoproteins

“…10 Over the past few years, various DBSA-derived fluorescent probes for Sec have been reported in a “turn-on” manner via Sec-triggered cleavage reaction of the sulfonamide bond that can remove the quenching effect of the strong electron-withdrawing nitro groups. 11 However, these probes may encounter interference of background autofluorescence in biological samples, which can largely impair the detection sensitivity towards the rare amount of Sec inside living systems. 12 Additionally, the limited photo-stability of organic fluorophores may raise concerns about photo-bleaching of the probes, resulting in low reproducibility of the Sec analysis.…”