Rapid detection of theophylline using aptamer-based nanopore thin film sensor

Feng, Silu; Che, Xiangchen; Que, Long; Chen, Changtian; Wang, Wei

doi:10.1109/icsens.2016.7808959

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…To obtain the mean transduction signal, the same sample was measured using a minimum of three sensors. 39…”

Section: F Measurement and Data Analysismentioning

confidence: 99%

3D printed microfluidic chip integrated with nanointerferometer for multiplex detection of foodborne pathogens

Feng,

Mo,

Song

2024

AIP Advances

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The current foodborne pathogen detection methods, such as culture-based methods, polymerase chain reaction, and optical and electrochemical biosensors with nucleic acid, have high sensitivity and selectivity. However, they are slow, expensive, and require well-trained operators. In this study, we utilized a 3D printer to develop a novel chip with an aptamer-based nanointerferometer capable of identifying four distinct foodborne pathogens: Listeria monocytogenes, Escherichia coli, Salmonella typhimurium, and Staphylococcus aureus. The aptamer sensor on the chip achieved a limit-of-detection of 10 colony forming unit (CFU)/ml. With its high sensitivity and specificity, this chip offers a cost-effective platform for distinguishing and screening different foodborne pathogens.

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“…To obtain the mean transduction signal, the same sample was measured using a minimum of three sensors. 39…”

Section: F Measurement and Data Analysismentioning

confidence: 99%

3D printed microfluidic chip integrated with nanointerferometer for multiplex detection of foodborne pathogens

Feng,

Mo,

Song

2024

AIP Advances

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“…The selective binding abilities of aptamers were employed to develop different sensors [ 6 , 7 ]. A variety of aptamer-based sensors for low-molecular-weight substrates have been developed recently including electrochemical [ 8 , 9 , 10 , 11 ] and optical [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase

Andrianova¹,

Комарова²,

Grudtsov³

et al. 2017

Sensors

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The electrochemical detection of interactions between aptamers and low-molecular-weight targets often lacks sensitivity. Signal amplification improves the detection of the aptamer-analyte complex; Bsm DNA polymerase was used to amplify the signal from the interaction of vanillin and its aptamer named Van_74 on an ion-sensitive field-effect transistor (ISFET)-based biosensor. The aptamer was immobilized on the ISFET sensitive surface. A short DNA probe was hybridized with the aptamer and dissociated from it upon vanillin addition. A free probe interacted with a special DNA molecular beacon initiated the Bsm DNA polymerase reaction that was detected by ISFET. A buffer solution suitable for both aptamer action and Bsm DNA polymerase activity was determined. The ISFET was shown to detect the Bsm DNA polymerase reaction under the selected conditions. Vanillin at different concentrations (1 × 10−6–1 × 10−8 M) was detected using the biosensor with signal amplification. The developed detection system allowed for the determination of vanillin, starting at a 10−8 M concentration. Application of the Bsm DNA polymerase resulted in a 15.5 times lower LoD when compared to the biosensor without signal amplification (10.1007/s00604-017-2586-4).

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Detection of plant hormone abscisic acid (ABA) using an optical aptamer-based sensor with a microfluidics capillary interface

Song

Chen

Che

et al. 2017

2017 IEEE 30th International Conference on Micro Electro Mechanical Systems (MEMS)

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Rapid detection of theophylline using aptamer-based nanopore thin film sensor

Cited by 4 publications

References 10 publications

3D printed microfluidic chip integrated with nanointerferometer for multiplex detection of foodborne pathogens

3D printed microfluidic chip integrated with nanointerferometer for multiplex detection of foodborne pathogens

Amplified Detection of the Aptamer–Vanillin Complex with the Use of Bsm DNA Polymerase

Detection of plant hormone abscisic acid (ABA) using an optical aptamer-based sensor with a microfluidics capillary interface

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