Detection of plant hormone abscisic acid (ABA) using an optical aptamer-based sensor with a microfluidics capillary interface

Song, Chao; Chen, Changtian; Che, Xiangchen; Wang, Wei; Que, Long

doi:10.1109/memsys.2017.7863418

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…A competitive immunoassay was developed, in which the anti-ABA antibody is functionalized on the surface of microbeads trapped in the microfluidic device, allowing for the detection of ABA using a labelled ABA-BSA conjugate. It was possible to detect ABA not only in buffer but also in real grape samples (both spiked and intrinsic), in concentrations ranging from 10 −11 to 10 −1 mg/mL, making this method more sensitive than any other ABA detection method reported in the literature [ 24 , 28 ]. Using HPLC, only ABA concentrations above 10 −5 mg/mL could be detected.…”

Section: Discussionmentioning

confidence: 99%

Competitive Immunoassay in a Microfluidic Biochip for In-Field Detection of Abscisic Acid in Grapes

Domingues,

Meirinho,

Rodrigues

et al. 2024

Biosensors

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Viticulture and associated products are an important part of the economy in many countries. However, biotic and abiotic stresses impact negatively the production of grapes and wine. Climate change is in many aspects increasing both these stresses. Routine sample retrievals and analysis tend to be time-consuming and require expensive equipment and skilled personnel to operate. These challenges could be overcome through the development of a miniaturized analytic device for early detection of grapevine stresses in the field. Abscisic acid is involved in several plant processes, including the onset of fruit ripening and tolerance mechanisms against drought stress. This hormone can be detected through a competitive immunoassay and is found in plants in concentrations up to 10−1 mg/mL. A microfluidic platform is developed in this work which can detect a minimum of 10−11 mg/mL of abscisic acid in buffer. Grape samples were tested using the microfluidic system alongside benchmark techniques such as high-performance liquid chromatography. The microfluidic system could detect the increase to 10−5 mg/mL of abscisic acid present in real berry samples at the veraison stage of ripening.

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Section: Discussionmentioning

confidence: 99%

Competitive Immunoassay in a Microfluidic Biochip for In-Field Detection of Abscisic Acid in Grapes

Domingues,

Meirinho,

Rodrigues

et al. 2024

Biosensors

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“…Song et al have reported an optical interferometric aptamer-based biosensor for sensitive and specific detection of plant hormone abscisic acid (ABA) on a microfluidic device [104]. As can be seen in Figure 17, the microfluidic chip consists of a nanopore-based sensing region and SU-8 microstructures as capillary microfluidics on both sides of the sensing region consisting of periodically distributed nanopores.…”

Section: Optical Interferometric Detectionmentioning

confidence: 99%

“…Optical interferometric detection-based biosensor: (A) schematic of the sensor chip; (B,C) enlarged view of the SU8 microstructures for microfluidics capillary interface; (D) the optical transducing signal from the nanopore-sensing region. Reprinted from[104], Copyright 2017, with permission from the 30th IEEE International Conference on Micro Electro Mechanical Systems (MEMS).…”

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confidence: 99%

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

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“…In recent years, microfluidic chips have aroused increasing interest for various application because of their desirable features, such as the smaller sample amount needed and lowered reagent consumption. The substrate materials of microfluidic chips such as polymers (e.g., PDMS, PMMA, PS) [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ], ceramics (e.g., glass) [ 6 , 13 , 14 , 16 , 17 , 18 , 19 , 21 , 25 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ], and semiconduct...…”

Section: Classification Of Microdevicesmentioning

confidence: 99%

Recent Microdevice-Based Aptamer Sensors

et al. 2018

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Since the systematic evolution of ligands by exponential enrichment (SELEX) method was developed, aptamers have made significant contributions as bio-recognition sensors. Microdevice systems allow for low reagent consumption, high-throughput of samples, and disposability. Due to these advantages, there has been an increasing demand to develop microfluidic-based aptasensors for analytical technique applications. This review introduces the principal concepts of aptasensors and then presents some advanced applications of microdevice-based aptasensors on several platforms. Highly sensitive detection techniques, such as electrochemical and optical detection, have been integrated into lab-on-a-chip devices and researchers have moved towards the goal of establishing point-of-care diagnoses for target analyses.

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Detection of plant hormone abscisic acid (ABA) using an optical aptamer-based sensor with a microfluidics capillary interface

Cited by 7 publications

References 10 publications

Competitive Immunoassay in a Microfluidic Biochip for In-Field Detection of Abscisic Acid in Grapes

Competitive Immunoassay in a Microfluidic Biochip for In-Field Detection of Abscisic Acid in Grapes

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Recent Microdevice-Based Aptamer Sensors

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