Rapid detection of staphylococcal enterotoxin B in milk samples based on fluorescence hybridization chain reaction amplification

Yan, Xu; Huo, Bingyang; Sun, Xuan; Ning, Baoan; Peng, Yuan; Bai, Jintao; Gao, Zhixian

doi:10.1039/c8ra01599f

Cited by 14 publications

(12 citation statements)

References 51 publications

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“…Some research groups have been studying on the combination of HCR and detection. Xu et al 24 modified the fluorophore and quencher groups on the hairpins, and opened the hairpins through the HCR to recover the fluorescence and realize the detection of staphylococcal enterotoxin B (SEB). Li et al 25 combined the fluorescence quenching of gold nanoparticles (AuNPs) and HCR amplification to detect anterior gradient homologue 2 (AGR2).…”

mentioning

confidence: 99%

Upconversion Fluorescent Aptasensor for Polychlorinated Biphenyls Detection Based on Nicking Endonuclease and Hybridization Chain Reaction Dual-Amplification Strategy

et al. 2018

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A novel upconversion fluorescent aptasensor based on hybridization chain reaction and nicking endonuclease has been developed for detection of polychlorinated biphenyls (PCBs). It combined the dual advantages of UCNPs and HCR. Two harpins (H1 and H2) were first designed according to the partial complementary sequence (cDNA) of the PCB72/106. Since the aptamer specifically recognized the target, the cDNA was detached from the magnetic microspheres (MMPs). The cDNA could initiate hybridization chain reaction (HCR) and open the stems of H1 and H2. After the addition of nicking endonuclease, UCNPs were further away from the quenchers (BHQ-1). Hence, the fluorescence intensity of upconversion nanoparticals (UCNPs) could be restored via fluorescence resonance energy transfer (FRET). Therefore, the fluorescence of UCNPs was directly proportional to concentration of PCB72/106, which was the basis for the quantification of PCB72/106. PCB72/106 could be analyzed within the ranges of 0.004 to 800 ng/mL with a detection limit of 0.0035 ng/mL ( S/ N = 3). The aptasensor was also used for the detection of water and soil samples, and the average recoveries ranged from 93.4% to 109.7% and 83.2% to 118.5%, respectively. The relative standard deviations (RSDs) were all below 3.2%. The signal was first amplified through HCR and further amplified with the help of nicking endonuclease. This work also provided the opportunity to develop fluorescent aptasensors for other targets using this dual-amplification strategy.

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confidence: 99%

Upconversion Fluorescent Aptasensor for Polychlorinated Biphenyls Detection Based on Nicking Endonuclease and Hybridization Chain Reaction Dual-Amplification Strategy

et al. 2018

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“…Thus considering these factors, chicken IgY antibodies offer several advantages over mammalian counterparts as they do not interact with rheumatoid factor (RF), human anti-mouse IgG antibodies (HAMA), complement components or mammalian Fc receptors 31 and thereby enhances the aptness of the developed platform for onsite applications. Several SEB detection assays, 23,24,48 as well as systems with IgY based strategies have been reported previously. [52][53][54] Despite establishment of such novel approaches and numerous improvements within these described assays, the commercially available kits still utilize polyclonal/monoclonal IgG antibodies based ELISA as represented in Table 8.…”

Section: Discussionmentioning

confidence: 99%

“…[20][21][22] Furthermore, SEB is one of the most potent potential agents of bioterrorism, despite its ban aer Biological and Toxin Weapons Convention (BWC) of 1972, it remains as serious concern that SEB could be used as bio-warfare agent. 23 The SEB toxin could also be aerosolized and its superantigenic nature leads to incapacitation of enemy forces. 24 It is therefore critical to develop countermeasures to prevent or treat the lethal and incapacitating effects of SEB.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of an IgY based silica matrix immunoassay platform for rapid onsite SEB detection

et al. 2018

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“…Nucleic acid-based amplification technologies, such as strand displacement reaction (SDR) [63][64][65], metal ion-dependent DNAzyme [66,67], hybridization chain reaction (HCR) [68][69][70], and nuclease cycling cleavage [71,72] remarkably enhanced the detection sensitivity. Zhao et al designed an ultrasensitive impedimetric CEA aptasensor based on the amplification effect of Zn 2+ -dependent DNAzyme-inspired cycling cleavage [73].…”

Section: Electrochemical Aptasensorsmentioning

confidence: 99%

“…(HCR) [68][69][70], and nuclease cycling cleavage [71,72] remarkably enhanced the detection sensitivity. Zhao et al designed an ultrasensitive impedimetric CEA aptasensor based on the amplification effect of Zn 2+ -dependent DNAzyme-inspired cycling cleavage [73].…”

Section: Electrochemical Aptasensorsmentioning

confidence: 99%

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

Jiang

Xia

Zang

et al. 2021

Sensors

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Recently, electrochemistry- and photoelectrochemistry-based biosensors have been regarded as powerful tools for trace monitoring of carcinoembryonic antigen (CEA) due to the fact of their intrinsic advantages (e.g., high sensitivity, excellent selectivity, small background, and low cost), which play an important role in early cancer screening and diagnosis and benefit people’s increasing demands for medical and health services. Thus, this mini-review will introduce the current trends in electrochemical and photoelectrochemical biosensors for CEA assay and classify them into two main categories according to the interactions between target and biorecognition elements: immunosensors and aptasensors. Some recent illustrative examples are summarized for interested readers, accompanied by simple descriptions of the related signaling strategies, advanced materials, and detection modes. Finally, the development prospects and challenges of future electrochemical and photoelectrochemical biosensors are considered.

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Rapid detection of staphylococcal enterotoxin B in milk samples based on fluorescence hybridization chain reaction amplification

Abstract: A new competitive aptasensor combined with HCR was developed for SEB detection.

Cited by 14 publications

References 51 publications

Upconversion Fluorescent Aptasensor for Polychlorinated Biphenyls Detection Based on Nicking Endonuclease and Hybridization Chain Reaction Dual-Amplification Strategy

Upconversion Fluorescent Aptasensor for Polychlorinated Biphenyls Detection Based on Nicking Endonuclease and Hybridization Chain Reaction Dual-Amplification Strategy

Development and evaluation of an IgY based silica matrix immunoassay platform for rapid onsite SEB detection

Electrochemistry/Photoelectrochemistry-Based Immunosensing and Aptasensing of Carcinoembryonic Antigen

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