Rapid detection of rotavirus in stool by latex agglutination: Comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test

Oj, Haikala; Jo, Kokkonen; Mk, Leinonen; Nurmi, T; Mäntyjärvi, R; Hk, Sarkkinen

doi:10.1002/jmv.1890110202

Cited by 44 publications

(30 citation statements)

References 24 publications

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“…Rotalex is rapid and does not require complicated or expensive equipment. We found Rotalex to be more sensitive than EM in accordance with previous reports (21,22). Nevertheless, its sensitivity has to be improved.…”

Section: Discussionsupporting

confidence: 92%

Comparison of six methods for detecting human rotavirus in stools

Morinet

Ferchal

Colimon

et al. 1984

Eur. J, Clin. Microbiol.

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The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost ) and a latex agglutination test ( Rotalex ). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex , 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost , 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex .

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Section: Discussionsupporting

confidence: 92%

Comparison of six methods for detecting human rotavirus in stools

Morinet

Ferchal

Colimon

et al. 1984

Eur. J, Clin. Microbiol.

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“…In addition, a variety of in vitro detection systems, including enzyme immunoassays (149,175,333,408), radioimmunoassays (137,141,181,264), latex agglutination (140,169), and nucleic acid hybridization techniques (116,175), have been developed for their rapid detection. The detection and identification of these agents are important since viral gastroenteritis is the second most common clinical entity in developed countries, second only to viral upper respiratory tract illness (188).…”

Section: Christensenmentioning

confidence: 99%

Human viral gastroenteritis

Christensen

1989

Clin Microbiol Rev

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During the last 15 years, several different groups of fastidious viruses that are responsible for a large proportion of acute viral gastroenteritis cases have been discovered by the electron microscopic examination of stool specimens. This disease is one of the most prevalent and serious clinical syndromes seen around the world, especially in children. Rotaviruses, in the family Reoviridae, and fastidious fecal adenoviruses account for much of the viral gastroenteritis in infants and young children, whereas the small caliciviruses and unclassified astroviruses, and possibly enteric coronaviruses, are responsible for significantly fewer cases overall. In addition to electron microscopy, enzyme immunoassays and other rapid antigen detection systems have been developed to detect rotaviruses and fastidious fecal adenoviruses in the stool specimens of both nonhospitalized patients and those hospitalized for dehydration and electrolyte imbalance. Experimental rotavirus vaccines have also been developed, due to the prevalence and seriousness of rotavirus infection. The small, unclassified Norwalk virus and morphologically similar viruses are responsible for large and small outbreaks of acute gastroenteritis in older children, adolescents, and adults. Hospitalization of older patients infected with these viruses is usually not required, and their laboratory diagnoses have been limited primarily to research laboratories.

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“…The grids were examined with a Jeol-Jem-100S electron microscope (Japanese Electron Optics, Beabody, Mass., U.S.A.) at a magnification of 40 000X. A latex agglutination (LA) method was used to detect rotavixus antigen (Haikala et al, 1983;Morinet et al, 1984): 20 u[ of the fecal suspension from each sample was mixed with an equal amount of LA reagent on a glass slide from each sample. Negative controls were also made.…”

Section: Virologymentioning

confidence: 99%

Certain enteropathogens in calves of Finnish dairy herds with recurrent outbreaks of diarrhea

Pohjola¹,

Oksanen²,

Neuvonen³

et al. 1986

Preventive Veterinary Medicine

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Pohjola, S., Oksanen, H., Neuvonen, E., Veijalainen, P. and Henriksson, K., 1986. Certain enteropathogens in calves of Finnish dairy herds with recurrent outbreaks of diarrhea. Prey. Vet. Med., 3: 547--558.The occurrence and distribution of certain enteropathogenic organisms and the manifestation of diarrhea, especially in relation to colostral management, were studied in neonatal calves of eight southern Finnish dairy herds with recurrent outbreaks of enteric disturbances. The fecal samples were obtained from the calves and were provided by the farmers. Potentially pathogenic agents were demonstrated in 52 of the 68 calves studied. Cryptosporidium was found in 36 of them while rotavirus was found in 29 and Escherichin coli in 15 of them, either singly or in combination. Salmonella, coronavirus or parvovirus were not detected in any of the calves. Twenty-six of the 36 calves contracted their Cryptosporidium infection and 18 of the 29 calves their rotavirus infection within the first week of life.Eighteen of the 52 infected calves developed diarrhea of microbial origin within 20 days of age; the most frequent finding was mixed infection with Cryptosporidium and rotavirus. In addition there were six diarrheic calves where no infection could be demonstrated. Only 10 of the 68 calves had neither infections nor diarrhea. There was a highly significant difference between the mean times of the first colostral feeding of the uninfected non-diarrheic and diarrheic calves and a significant difference between the mean times of the first colostral feeding of the subclinically infected and diarrheic calves. The importance of sucking of colostrum at the first feeding was observed. The smallest amount protecting the calves from infections was 0.75 1 given within 1 h of delivery.

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Rapid detection of rotavirus in stool by latex agglutination: Comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test

Cited by 44 publications

References 24 publications

Comparison of six methods for detecting human rotavirus in stools

Comparison of six methods for detecting human rotavirus in stools

Human viral gastroenteritis

Certain enteropathogens in calves of Finnish dairy herds with recurrent outbreaks of diarrhea

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