Rapid Detection of HSV from Cytologic Specimens Collected into ThinPrep Fixative

Fiel-Gan, Mary; Villamil, Carlos F.; Mandavilli, Srinivas; Ludwig, Mark; Tsongalis, Gregory J.

doi:10.1159/000331350

Cited by 17 publications

(12 citation statements)

References 14 publications

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“…This study provides additional evidence that a single cervical specimen can be used for cervical cytology and screening for C. trachomatis and N. gonorrhoeae using nucleic acid amplification (1), even among these adolescents, who theoretically may have had fewer cellular PreservCyt samples. The advantages of a single specimen include the need for only one collection device, the preservation of cellular material and nucleic acid in one liquid medium, and the ability to detect not only C. trachomatis and N. gonorrhoeae but other vaginal-cervical infections as well (5,9). If these results are confirmed, providers and women could benefit from the simplicity and versatility of using a single specimen for many recommended routine screening activities.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of Methods for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Commercially Available Nucleic Acid Amplification Tests and a Liquid Pap Smear Medium

Koumans

Black²,

Markowitz³

et al. 2003

J Clin Microbiol

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Annual screening for Chlamydia trachomatis infection is currently recommended for sexually active women 15 to 25 years old and for women older than 25 if they have a new or multiple sex partners and have not used condoms during the previous 3 months. Annual screening for cervical abnormalities using the Pap smear has achieved a substantial reduction in morbidity and mortality from cervical cancer. Screening for Neisseria gonorrhoeae infection has likely contributed significantly to the reduction in the rates of gonococcal infection. The introduction of liquid Pap smear methods using exfoliated cervical cells presents an opportunity to screen for these three conditions using one specimen. We evaluated the preservation of C. trachomatis and Neisseria gonorrhoeae DNAs from ThinPrep liquid media (PreservCyt; Cytyc Corp., Boxborough, Mass.); tested the feasibility of using a clinical specimen of this medium for the detection of cytologic abnormalities, C. trachomatis, and N. gonorrhoeae; evaluated the agreement between ligase chain reaction (LCR) performed on PreservCyt and LCR performed on a cervical specimen; and compared the performance of LCR performed on PreservCyt to those of LCR performed on a cervical specimen, culture, PCR performed on a cervical specimen, on urine, and on a vaginal specimen (a multiple-site infection status standard), and transcription-mediated amplification (for C. trachomatis only) from 255 sexually active adolescent women. The agreement between LCR performed on PreservCyt and LCR from a cervical swab in LCx transport medium was high (for C. trachomatis, agreement ‫؍‬ 0.97 and kappa ‫؍‬ 0.92; for N. gonorrhoeae, agreement ‫؍‬ 0.99 and kappa ‫؍‬ 0.96). Test performances were similar for LCR-urine, LCR-cervix, and LCR-ThinPrep, with sensitivities from 93 to 99% for C. trachomatis and 81 to 83% for N. gonorrhoeae and specificities from 95.5 to 99% for C. trachomatis and 99.1 to 99.6% for N. gonorrhoeae using a PCR-based multiple-site infection status standard. This is the first study to examine the agreement between liquid cytologic media and multiple nucleic acid amplification tests for the detection of C. trachomatis and N. gonorrhoeae from patient samples. Cytologic fluid shows promise for simultaneous screening for cytologic abnormalities and sexually transmitted infections.

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Section: Discussionmentioning

confidence: 99%

“…C. trachomatis has been detected using both a direct fluorescence assay (9) and nucleic acid amplification (13); human papillomavirus (HPV) has been detected using PCR (12) and the Hybrid Capture test (Digene Corp., Gaithersberg, Md.) (14); herpes simplex virus has been detected using PCR (5).…”

mentioning

confidence: 99%

Comparison of Methods for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Commercially Available Nucleic Acid Amplification Tests and a Liquid Pap Smear Medium

Koumans

Black²,

Markowitz³

et al. 2003

J Clin Microbiol

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“…In clinical practice, HPV is currently detected by molecular methods (in situ hybridization, Hybrid Capture Test, or PCR) to identify women infected with high-risk HPVs or women with equivocal cervical lesions (50). Finally, a recent study has demonstrated that PCR methods can be used to detect herpes simplex virus in ThinPrep cervical cytologic specimens and that this test is useful when the microcopic diagnosis is equivocal (13). This is the first evaluation of the COBAS Amplicor CT/NG test for detection of C. trachomatis by the fluid-based ThinPrep Pap method.…”

Section: Discussionmentioning

confidence: 99%

PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications

Bianchi¹,

Moret

Desrues³

et al. 2002

J Clin Microbiol

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The commercial COBAS Amplicor CT/NG test (Roche Diagnostic Systems, Meylan, France) is a sensitive and specific method for detection of Chlamydia trachomatis infections. This test currently consists of using a nucleic acid amplification method to detect C. trachomatis in first-void urine specimens and in endocervical swabs collected in 2-sucrose-phosphate (2SP) transport medium. We conducted a prospective study to determine whether the automated COBAS Amplicor CT/NG test can detect C. trachomatis in cervical specimens collected in PreservCyt transport medium (ThinPrep Pap Test; Cytyc Corporation, Boxborough, Mass.). PreservCyt medium is used to preserve cervical samples before the preparation of ThinPrep slides. We collected 1,000 cervical specimens from young women (age range, 15 to 25 years) during routine Pap smear tests. Only specimens with normal cytology and in which the gynecologist found no clinical evidence of urogenital infections were selected. The samples were stored in PreservCyt transport medium at 15 to 20°C. C. trachomatis was detected in 22 of the 1,000 cervical specimens that had been stored in PreservCyt. To confirm the positive samples, the test was repeated on new endocervical swab specimens collected in 2SP transport medium. Only 9 of the 22 positive patients agreed to undergo this control, but all 9 retested positive. To evaluate the influence of storage conditions on the sensitivity of the C. trachomatis PCR test, all of the positive samples were stored at 15 to 20°C in PreservCyt transport medium and were retested every 2 weeks for 6 weeks. C. trachomatis was successfully amplified from all 22 specimens for the whole 6-week period. The prevalence of C. trachomatis infection was 2.2% in our study population. These results demonstrate that PreservCyt transport medium is a suitable transport medium for detection of C. trachomatis by the COBAS Amplicor CT/NG test. The ThinPrep Pap Test may enable gynecologists to monitor for both cervical lesions and C. trachomatis infections with a single endocervical specimen.Chlamydia trachomatis infection is one of the most common sexually transmitted diseases and represents a major public health problem (48). In women, untreated C. trachomatis infections cause cervicitis and pelvic inflammatory disease, which may lead to infertility and ectopic pregnancy, and expose newborn infants to C. trachomatis (37). In the early 1990s, molecular diagnostic methods based on nucleic acid amplification tests such as PCR, ligase chain reaction, and transcriptionmediated amplification were developed. These techniques have gradually replaced traditional techniques for detection of C. trachomatis (5,32,49). Commercial DNA amplification tests are now routinely used for diagnosis of C. trachomatis infections. Although these commercial tests are based on different nucleic acid amplification technologies, many studies have shown that nucleic acid amplification-based tests are more sensitive than culture-based tests (17,28,33,35,39,40) and that they offer the opportunity to u...

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“…The residual sample of LBPTs, which is commonly used to test for human papillomavirus DNA, can also be used for the detection of other agents infecting the lower genital tract. Recent studies have demonstrated successful detection of Chlamydia and gonococci, 6 Trichomonas, 7 and even herpes simplex virus (HSV) 8 in residual LBPTs. Genital HSV infection is a highly prevalent latent infection in women which is usually caused by herpes simplex virus type 2 (HSV-2).…”

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confidence: 99%

Accuracy of herpes simplex virus detection in liquid‐based (SurePath) Papanicolaou tests: A comparison with polymerase chain reaction

Aslan

Pambuccian

Prekker

et al. 2008

Diagnostic Cytopathology

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A review of our institution's Papanicolaou test records over an 11-yr period showed that liquid-based Papanicolaou tests (LBPTs) had a significantly higher frequency of diagnoses of Herpes simplex virus (HSV)-related cellular changes compared to conventional Papanicolaou smears (77/302,841, 0.026% vs. 56/376,173, 0.015%, P = 0.002). To investigate the accuracy of the diagnosis of HSV by LBPT, we performed conventional polymerase chain reaction (PCR) on the residual samples from 258 prospectively collected LBPT and real-time PCR using a different primer set on a subset of 40 LBPT. Conventional PCR was positive in 22 of 22 cases diagnosed of HSV, 1 of 2 cases diagnosed as suspicious for HSV, and none of 234 LBPT without a cytologic HSV diagnosis. Real-time PCR was positive in 8 of 8 cases diagnosed as HSV and none of the 32 controls. We conclude that LBPT allows an increased detection of HSV that is highly accurate.

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Rapid Detection of HSV from Cytologic Specimens Collected into ThinPrep Fixative

Cited by 17 publications

References 14 publications

Comparison of Methods for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Commercially Available Nucleic Acid Amplification Tests and a Liquid Pap Smear Medium

Comparison of Methods for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Commercially Available Nucleic Acid Amplification Tests and a Liquid Pap Smear Medium

PreservCyt Transport Medium Used for the ThinPrep Pap Test Is a Suitable Medium for Detection of Chlamydia trachomatis by the COBAS Amplicor CT/NG Test: Results of a Preliminary Study and Future Implications

Accuracy of herpes simplex virus detection in liquid‐based (SurePath) Papanicolaou tests: A comparison with polymerase chain reaction

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