Rapid detection of “highly virulent” Group B Streptococcus ST-17 and emerging ST-1 clones by MALDI-TOF mass spectrometry

Lartigue, Marie‐Frédérique; Kostrzewa, Markus; Salloum, Mazen; Haguenoer, Eve; Héry-Arnaud, Geneviève; Domelier, Anne-Sophie; Stumpf, Simone; Quentin, Roland

doi:10.1016/j.mimet.2011.05.017

Cited by 43 publications

(60 citation statements)

References 9 publications

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“…Consistent with previous results, MALDI-TOF MS was able to identify each of the isolates with high confidence. Moreover, the technique was also able to discriminate highly virulent isolates of the ST-17 and ST-1 serotypes from other isolates with strong predictive values, indicating the potential of this method for future use with respect to strain identification and infection management in clinical practice (104).…”

Section: Streptococcimentioning

confidence: 91%

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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SUMMARY Within the past decade, clinical microbiology laboratories experienced revolutionary changes in the way in which microorganisms are identified, moving away from slow, traditional microbial identification algorithms toward rapid molecular methods and mass spectrometry (MS). Historically, MS was clinically utilized as a high-complexity method adapted for protein-centered analysis of samples in chemistry and hematology laboratories. Today, matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS is adapted for use in microbiology laboratories, where it serves as a paradigm-shifting, rapid, and robust method for accurate microbial identification. Multiple instrument platforms, marketed by well-established manufacturers, are beginning to displace automated phenotypic identification instruments and in some cases genetic sequence-based identification practices. This review summarizes the current position of MALDI-TOF MS in clinical research and in diagnostic clinical microbiology laboratories and serves as a primer to examine the “nuts and bolts” of MALDI-TOF MS, highlighting research associated with sample preparation, spectral analysis, and accuracy. Currently available MALDI-TOF MS hardware and software platforms that support the use of MALDI-TOF with direct and precultured specimens and integration of the technology into the laboratory workflow are also discussed. Finally, this review closes with a prospective view of the future of MALDI-TOF MS in the clinical microbiology laboratory to accelerate diagnosis and microbial identification to improve patient care.

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Section: Streptococcimentioning

confidence: 91%

“…Two studies from Lartigue et al focused exclusively on GBS identification by MALDI-TOF MS analysis (103,104). The first study investigated the ability of MALDI-TOF MS to identify 110 isolates of molecularly and serologically characterized GBS using cellular extracts.…”

Section: Streptococcimentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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“…Today, development is ongoing for a rapid cheap test that would allow the detection of ST-17 marker among GBS isolates at time of culture. Routinely in many laboratories MALDI-TOF mass spectrometry is an effective tool used for bacterial identification; a study has suggested that use of appropriate analysis software for GBS submitted to analysis by may be able to identify simultaneously whether or not strains belong to this ST-17 clone [54]. Clinical studies are needed to confirm the robustness of the detection of the ST-17 clones by this new technical approach and to evaluate the clinical impact on current prevention strategy, on the management and follow-up of neonates.…”

Section: Description Of the Bacteriamentioning

confidence: 99%

Intrapartum GBS screening and antibiotic prophylaxis: a European consensus conference

Renzo

Melin²,

Berardi

et al. 2014

The Journal of Maternal-Fetal & Neonatal Medicine

148

135

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Group B streptococcus (GBS) remains worldwide a leading cause of severe neonatal disease. Since the end of the 1990s, various strategies for prevention of the early onset neonatal disease have been implemented and have evolved. When a universal antenatal GBS screening-based strategy is used to identify women who are given an intrapartum antimicrobial prophylaxis, a substantial reduction of incidence up to 80% has been reported in the USA as in other countries including European countries. However recommendations are still a matter of debate due to challenges and controversies on how best to identify candidates for prophylaxis and to drawbacks of intrapartum administration of antibiotics. In Europe, some countries recommend either antenatal GBS screening or risk-based strategies, or any combination, and others do not have national or any other kind of guidelines for prevention of GBS perinatal disease. Furthermore, accurate population-based data of incidence of GBS neonatal disease are not available in some countries and hamper good effectiveness evaluation of prevention strategies. To facilitate a consensus towards European guidelines for the management of pregnant women in labor and during pregnancy for the prevention of GBS perinatal disease, a conference was organized in 2013 with a group of experts in neonatology, gynecologyobstetrics and clinical microbiology coming from European representative countries. The group reviewed available data, identified areas where results were suboptimal, where revised procedures and new technologies could improve current practices for prevention of perinatal GBS disease. The key decision issued after the conference is to recommend intrapartum antimicrobial prophylaxis based on a universal intrapartum GBS screening strategy using a rapid real time testing.

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“…ST-17 has been shown to be associated with GBS LOD and meningitis (12). In addition, the sequence types ST17 and ST10 are associated with meningitis, and sequence types ST1 and ST23 are associated with septicemia (13,14). U n C o r r e c t e d P r o o f tal procedure, thereby producing an accurate picture of GBS occurrence in pregnant women and infants.…”

Section: Introductionmentioning

confidence: 99%

Serotype, Genotype, and Clinical Manifestations of Group B Streptococcus (GBS) Isolated from Neonates in China

Wang

et al. 2018

Iran J Pediatr

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Background: Group B streptococcus (GBS) is a common bacterial pathogen causing nenatal infections. This study aimed to determine the serotypes and genotypes of GBS isolated from neonates and to ascertain the association between the type and the antibiotic resistance of GBS strains. Methods: A total of 26 group B streptococcus (GBS) strains were isolated from neonates in our hospital between January, 2008 and August, 2014. Species identification was conducted with a VITEK system (BioMerieux) and conventional biochemical tests. A latex agglutination test was used to determine the serotypes of the GBS strains. The antibiotic resistance of GBS strains was then evaluated using a Kirby-Bauer disk susceptibility test and an Epsilometer test (E-test). Multilocus sequence typing (MLST) was used to determine the sequence types (STs) of all 26 GBS strains. Results and Conclusions: Serotype III and sequence type ST17 were the major GBS strain serotype and genotype causing invasive infections in neonates, respectively. These GBS strains exhibited a high rate of resistance to tetracycline, erythromycin, and clindamycin, confirming that penicillin should remain the first-line antibiotic for the treatment of GBS infection. In addition, we identified an association between serotype III and genotype ST17 with septicaemia, purulent meningitis and pneumonia in GBS infections in neonates.

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Rapid detection of “highly virulent” Group B Streptococcus ST-17 and emerging ST-1 clones by MALDI-TOF mass spectrometry

Cited by 43 publications

References 9 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Intrapartum GBS screening and antibiotic prophylaxis: a European consensus conference

Serotype, Genotype, and Clinical Manifestations of Group B Streptococcus (GBS) Isolated from Neonates in China

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