Rapid Detection of Highly Pathogenic Avian Influenza H5N1 Virus by TaqMan Reverse Transcriptase-Polymerase Chain Reaction

Shankar, B. P.; Gowda, R. N. S.; Pattnaik, Bramhadev; Prabhu, B. H. M.; Patil, S. S.; Pradhan, H.K.

doi:10.3923/ijps.2009.260.263

Cited by 2 publications

(1 citation statement)

References 6 publications

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“…In 2007, Chen et al also described a sensitive and specific RRT-PCR method for the detection of influenza A subtype H5N1 [26]. Recently, a TaqMan assay was designed by using two sets of primers and probes to amplify two different regions of the HA gene of HPAI H5N1 virus [29]. Their results showed that the assay is specific for the H5 subtype and capable of detecting H5 RNA samples obtained from infected birds.…”

Section: Pcr-based Methodsmentioning

confidence: 99%

Methods for molecular surveillance of influenza

Wang

Taubenberger

2010

Expert Review of Anti-infective Therapy

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Molecular-based techniques for detecting influenza viruses have become an integral component of human and animal surveillance programs in the last two decades. The recent pandemic of the swineorigin influenza A virus (H1N1) and the continuing circulation of highly pathogenic avian influenza A virus (H5N1) further stress the need for rapid and accurate identification and subtyping of influenza viruses for surveillance, outbreak management, diagnosis and treatment. There has been remarkable progress on the detection and molecular characterization of influenza virus infections in clinical, mammalian, domestic poultry and wild bird samples in recent years. The application of these techniques, including reverse transcriptase-PCR, real-time PCR, microarrays and other nucleic acid sequencing-based amplifications, have greatly enhanced the capability for surveillance and characterization of influenza viruses.

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Section: Pcr-based Methodsmentioning

confidence: 99%