Rapid detection of Escherichia coli using fiber optic surface plasmon resonance immunosensor based on biofunctionalized Molybdenum disulfide (MoS2) nanosheets

Kaushik, Siddharth; Tiwari, Umesh; Pal, Sudipta Sarkar; Sinha, R. K.

doi:10.1016/j.bios.2018.11.006

Cited by 169 publications

(84 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present research outlines a novel method for biofunctionalization and sensitivity enhancement of the optical fiber SPR sensor for biosensing applications. The interfacing of MoS 2 sheets facilitates simple and rapid antibodies immobilization on transducer’s surface via a convenient method of hydrophobic interaction 57 . In this way, the developed sensor avoids the requirement of any harsh chemical or muti-step treatments to the fiber which affects the sensitivity of the sensor by forming additional layer between antibodies and sensing probe.…”

Section: Introductionmentioning

confidence: 99%

Two-dimensional transition metal dichalcogenides assisted biofunctionalized optical fiber SPR biosensor for efficient and rapid detection of bovine serum albumin

Kaushik

Tiwari

Deep

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

The present study reports an alternative method of functionalizing the optical fiber Surface Plasmon Resonance (SPR) sensing probe with antibodies for label-free detection of bovine serum albumin (BSA) protein. In this novel approach, the gold coated fiber was first modified with Molybdenum disulfide (MoS 2 ) nanosheets followed by its bio-functionalization with Anti-BSA antibodies. The developed technique not only allowed the amplification of the SPR signals by synergic effects of MoS 2 and gold metallic thin film but also enabled a direct and chemical-free attachment of representative antibodies through hydrophobic interactions. The sensitivity of the MoS 2 modified sensing probe with detection limit of 0.29 µg/mL was improved as compared to the fiber optic SPR biosensor without MoS 2 overlayer (Detection limit for BSA was 0.45 μg/mL). The developed biosensor has good specificity, and environmental stability. Accordingly, the proposed design of the MoS 2 based SPR optical biosensor can offer the development of a simplified optical device for the monitoring of various biomedical and environmental parameters.

show abstract

Section: Introductionmentioning

confidence: 99%

Two-dimensional transition metal dichalcogenides assisted biofunctionalized optical fiber SPR biosensor for efficient and rapid detection of bovine serum albumin

Kaushik

Tiwari

Deep

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Because only the cladding mode interacts with the bacteria, their presence provides the phase shift and resulting interference signal. An LOD of only 7 CFU/mL of E. coli was reported [72], which highlights the remarkable sensitivity of this technique.…”

Section: Optical Waveguides and Interferometric Approachesmentioning

confidence: 83%

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

2020

View full text Add to dashboard Cite

Photonic biosensors are a major topic of research that continues to make exciting advances. Technology has now improved sufficiently for photonics to enter the realm of microbiology and to allow for the detection of individual bacteria. Here, we discuss the different nanophotonic modalities used in this context and highlight the opportunities they offer for studying bacteria. We critically review examples from the recent literature, starting with an overview of photonic devices for the detection of bacteria, followed by a specific analysis of photonic antimicrobial susceptibility tests. We show that the intrinsic advantage of matching the optical probed volume to that of a single, or a few, bacterial cell, affords improved sensitivity while providing additional insight into single-cell properties. We illustrate our argument by comparing traditional culture-based methods, which we term macroscopic, to microscopic free-space optics and nanoscopic guided-wave optics techniques. Particular attention is devoted to this last class by discussing structures such as photonic crystal cavities, plasmonic nanostructures and interferometric configurations. These structures and associated measurement modalities are assessed in terms of limit of detection, response time and ease of implementation. Existing challenges and issues yet to be addressed will be examined and critically discussed.

show abstract

“…In 70 nm and 100 nm Au-coated optical fiber, the SPR effect is predominant, and it can be used to detect bacteria at concentrations as low as 1.5 × 10 3 CFU/mL. Kaushik et al developed a molybdenum disulfide (MoS 2 ) nanosheets functionalized optic fiber to enhance the signal of an SPR immunosensor for the sensitive detection of E. coli [74] (Figure 6b). The two-dimensional nanosheet (MoS 2 ) was anchored to the surface of the Au coated optic fiber and monoclonal antibodies were immobilized on the functionalized nanosheets via hydrophobic interactions.…”

Section: Analytical Methods For Pathogenic Microbesmentioning

confidence: 99%

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Choi

Lee

Son

et al. 2020

Sensors

View full text Add to dashboard Cite

For the early diagnosis of several diseases, various biomarkers have been discovered and utilized through the measurement of concentrations in body fluids such as blood, urine, and saliva. The most representative analytical method for biomarker detection is an immunosensor, which exploits the specific antigen-antibody immunoreaction. Among diverse analytical methods, surface plasmon resonance (SPR)-based immunosensors are emerging as a potential detection platform due to high sensitivity, selectivity, and intuitive features. Particularly, SPR-based immunosensors could detect biomarkers without labeling of a specific detection probe, as typical immunosensors such as enzyme-linked immunosorbent assay (ELISA) use enzymes like horseradish peroxidase (HRP). In this review, SPR-based immunosensors utilizing noble metals such as Au and Ag as SPR-inducing factors for the measurement of different types of protein biomarkers, including viruses, microbes, and extracellular vesicles (EV), are briefly introduced.

show abstract

Rapid detection of Escherichia coli using fiber optic surface plasmon resonance immunosensor based on biofunctionalized Molybdenum disulfide (MoS2) nanosheets

Cited by 169 publications

References 53 publications

Two-dimensional transition metal dichalcogenides assisted biofunctionalized optical fiber SPR biosensor for efficient and rapid detection of bovine serum albumin

Two-dimensional transition metal dichalcogenides assisted biofunctionalized optical fiber SPR biosensor for efficient and rapid detection of bovine serum albumin

Nanophotonics for bacterial detection and antimicrobial susceptibility testing

Noble Metal-Assisted Surface Plasmon Resonance Immunosensors

Contact Info

Product

Resources

About