Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor

Seo, Gon; Lee, Geonhee; Kim, Mi Jeong; Baek, Seung Hwa; Choi, Minsuk; Ku, Keun Bon; Lee, Chang Seop; Jun, Sangmi; Park, Daeui; Kim, Hong Gi; Kim, Seong Jun; Lee, Jeong O.; Kim, Bum Tae; Park, Edmond Changkyun; Kim, Kyung Sik

doi:10.1021/acsnano.0c02823

Cited by 1,495 publications

(1,635 citation statements)

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“…Compared with reported studies, the LOD of the real-time PCR detection kit was better than other NAT assay or methods. [21][22][23][24] The precision analysis showed that CVs of two different concentrations of reference samples were all less than 5%, indicating a stable testing performance, especially on the detection of samples with low viral load. Therefore, the real-time PCR detection kit was with good performance and suitable for daily work in blood establishments based on available blood screening instruments.…”

Section: Discussionmentioning

confidence: 99%

No evidence of SARS‐CoV‐2 RNA among blood donors: A multicenter study in Hubei, China

et al. 2020

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Background Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) RNA could be detected in the blood of infected cases. From February 9, all blood establishments in Hubei province, China, implemented nucleic acid testing (NAT) for SARS‐CoV‐2 RNA among blood donors to ensure blood safety. Study Design and Methods Nucleic acid test screening individually (ID) or by minipool (MP) testing was performed according to the manufacturerʼs instructions. Inactivated culture supernatant of SARS‐CoV‐2–infected Vero cells was quantified by droplet digital polymerase chain reaction (ddPCR) and series diluted with negative plasma to evaluate the assayʼs performance. Results The limit of detection of the kit for MP testing was 62.94 and 33.14 copies/mL for N and ORF1ab region, respectively. ID testing could achieve 3.87 and 4.85 copies/mL for two regions using 1600 μL of plasma. Coefficients of variations of two different concentrations of reference samples were all less than 5% in MP testing. As of April 30, 2020, a total of 98,342 blood donations including 87,095 whole blood donations and 11,247 platelet donations were tested by ID or MP testing, and no RNAemia was found. In addition, Hubei province suffered precipitously decreased blood supply, especially in February: 86% reduction compared with the same period of 2019. Conclusion Nucleic acid test screening of SARS‐CoV‐2 on blood donations is suitable in blood establishments using the commercial real‐time PCR detection kit based on available instruments. The negative result indicated that SARS‐CoV‐2 appears to be no direct threat to blood safety but raises some serious issues for general blood supply.

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Section: Discussionmentioning

confidence: 99%

No evidence of SARS‐CoV‐2 RNA among blood donors: A multicenter study in Hubei, China

et al. 2020

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“…A direct comparison with other proteins is not possible as the exact charge distribution near to the silicon channel is unknown and highly likely to be completely different. However it is interesting to compare our results with reported work on a graphene TFT binding to the same spike protein [11]. There are two major differences in the experimental technique compared to the results presented here.…”

Section: Resultsmentioning

confidence: 72%

“…Most systems reported in the literature [11,15], use a small source drain potential to determine the response of the system. These small potentials result in relatively small shifts in the source drain current, which is used to detect the presence of the target.…”

Section: Resultsmentioning

confidence: 99%

“…The constant R, is an important observation as unlike other aptamer or antibody-based transistor sensors where the source drain potential is kept at a maximum of 100 mV [11,15,16], here a range of voltages was investigated. At low potentials, a wide variation of R can be seen as effectively, the numbers are small.…”

Section: Resultsmentioning

confidence: 99%

“…Once the target is attached to the antibody, the charged areas of the protein will cause an electrostatic change in the conduction channel of the FET and hence modulate the source-drain current. Such a device and concept has already been demonstrated for potential COVID-19 detection [11].…”

Section: Introductionmentioning

confidence: 99%

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Silicon thin film transistor-based aptamer sensor for COVID-19 detection

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Laumier

Sandall

et al. 2020

Preprint

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Since the beginning of the coronavirus disease 2019 (COVID-19) in December 2019 and the current lack to date of specific drugs or vaccinations to cope with the disease, it has become apparent that the surest way of dealing with this is through early diagnosis and management. Current testing has shown to be unable to rapidly and accurately provide the results required to restrict the spread. Here we report the use of an intrinsic silicon thin film transistor functionalised with aptamers designed to attach to the spike protein of COVID-19. It is shown that a linear response can be obtained in a concentration of 10 fM and 10 pM, and that the relative response is independent of the applied potential allowing a sensory system to fine tune the applied potential to facilitate the interpretation of the results.

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