Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk

Cell junctions serve as a protective barrier for cells and provide an important channel for information transmission between cells and the surrounding environment. Viruses are parasites that invade and commandeer components of host cells in order to survive and replicate, and they have evolved various mechanisms to alter cell junctions to facilitate viral infection. In this review, we examined the current state of knowledge on the action of viruses on host cell junctions. The existing evidence suggests that targeting the molecules involved in the virus‐cell junction interaction can prevent the spread of viral diseases.

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“…45 Thus, disruption of tight junctions may play an important role in the pathogenesis diarrhea caused by RV. 46…”

Section: Rotavirus (Rv) and Tight Junctionsmentioning

confidence: 99%

“…RhoA and its downstream effector Rho kinase (ROCK) are key molecules that mediate destruction of tight junction when RV infection . Thus, disruption of tight junctions may play an important role in the pathogenesis diarrhea caused by RV …”

Section: Introductionmentioning

confidence: 99%

Alteration of cell junctions during viral infection

2020

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“…With the development of carbon-based nanomaterials, mesoporous carbon (MC) have aroused remarkable interest in electrochemical detection fields because of distinct structural characteristics such as a large active surface area and pore volume, great conductivity, and outstanding stability in the terms of mechanical and thermal factors [ 54 , 55 ]. Some papers have been proved that mesoporous carbon can be used as a favorable position for electron transfer and adsorption position in electrochemical sensors owing to its vacancy defects [ 56 , 57 ].…”

Section: Application Of Carbon Nanomaterials In the Determination mentioning

confidence: 99%

Application of Electrochemical Sensors Based on Carbon Nanomaterials for Detection of Flavonoids

Zhang

2020

Nanomaterials

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Flavonoids have a variety of physiological activities such as anti-free radicals, regulating hormone levels, antibacterial factors, and anti-cancer factors, which are widely present in edible and medicinal plants. Real-time detection of flavonoids is a key step in the quality control of diverse matrices closely related to social, economic, and health issues. Traditional detection methods are time-consuming and require expensive equipment and complicated working conditions. Therefore, electrochemical sensors with high sensitivity and fast detection speed have aroused extensive research interest. Carbon nanomaterials are preferred material in improving the performance of electrochemical sensing. In this paper, we review the progress of electrochemical sensors based on carbon nanomaterials including carbon nanotubes, graphene, carbon and graphene quantum dots, mesoporous carbon, and carbon black for detecting flavonoids in food and drug homologous substances in the last four years. In addition, we look forward to the prospects and challenges of this research field.

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“…These results were analyzed by corresponding Ct values and melting curve. Additionally, the positive samples detected by real-time RT-PCR were confirmed by a rapid recombinase polymerase amplification in combination with a lateral flow dipstick (RPA-LFD) for BVDV to verify the accuracy and reliability of testing results [8].…”

Section: Performance Of Real-time Rt-pcr and Rt-pcr Assay On Aerosolmentioning

confidence: 99%

“…The genome is a single-stranded, positive-sense RNA molecule consisting of a 5′ untranslated region (5′-UTR), a single open reading frame (ORF) encoding structural proteins and non-structural proteins, and a 3′-UTR [7]. The 5′-UTR is most often targeted for molecular diagnosis technology and genotyping since it is highly conserved in the pestivirus genome [8][9][10][11]. Commonly, BVDV strains can be categorized into BVDV-1 and BVDV-2, and each genotype has been further divided into distinct subtypes [12][13][14][15].…”

mentioning

confidence: 99%

Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay

Hou

Wang

et al. 2020

BMC Vet Res

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Background: As a pestivirus of the Flaviviridae family, bovine viral diarrhea virus (BVDV), has imposed a large burden on animal husbandry worldwide, and such virus can be transmitted mainly through direct contact with other infected animals and probably via aerosols. In the present study, we aimed to develop a real-time RT-PCR method for detection of BVDV-1 in aerosol samples.Methods: A pair of primers specific for highly conserved regions of the BVDV-1 5′-UTR was designed. The standard curve and sensitivity of the developed assay were assessed based on 10-fold serial dilutions of RNA molecular standard. The specificity of the assay was evaluated with other pestiviruses and infectious bovine viruses. The clinical performance was examined by testing 169 aerosol samples. Results: The results showed that a good linear relationship existed between the standard curve and the concentration of template. The lowest detection limit was 5.2 RNA molecules per reaction. This assay was specific for detection of BVDV-1, and no amplification was found for other pestiviruses such as classical swine fever virus (CSFV), border disease virus (BDV), and common infectious bovine viruses, including BVDV-2, infectious bovine rhinotracheitis virus (IBRV), bovine parainfluenza virus type 3 (BPIV-3), bovine respiratory syncytial virus (BRSV), bovine ephemeral fever virus (BEFV) and bovine coronavirus (BcoV). The assay was highly reproducible with low variation coefficient values (CVs) for intra-assay and inter-assay. A total of 169 aerosol samples collected from six dairy herds were tested using this method. The results showed that the positive detection rate of BVDV-1 was 17.2% (29/169), which was significantly higher compared with the conventional RT-PCR. Additionally, the positive samples (n = 29) detected by real-time RT-PCR were verified by BVDV RPA-LFD, and a concordance rate of 100% was obtained between them. Conclusions:Taken together, we developed a real-time RT-PCR assay for quantitative analysis of BVDV-1 in aerosol samples, and our finding provided valuable insights into the risk on aerosol transmission of BVDV-1.

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Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk

Abstract: He: Rapid detection of bovine viral diarrhea virus using recombinase polymerase amplification combined with lateral flow dipstick assays in bulk milk. Vet. arhiv 88, 627-642, 2018.

Cited by 12 publications

References 33 publications

Alteration of cell junctions during viral infection

Alteration of cell junctions during viral infection

Application of Electrochemical Sensors Based on Carbon Nanomaterials for Detection of Flavonoids

Detection of bovine viral diarrhea virus genotype 1 in aerosol by a real time RT-PCR assay

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