Rapid detection of Bombyx mori nucleopolyhedrovirus (BmNPV) by loop-mediated isothermal amplification assay combined with a lateral flow dipstick method

Zhou, Yang; Jie-ge, WU; Lin, Feng; Chen, Nai-Fu; Yuan, Shaofei; Ding, Lei; Gao, Li; Han, Bangxing

doi:10.1016/j.mcp.2015.09.003

Cited by 16 publications

(1 citation statement)

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“…A similar approach was presented in order to establish a quick, sensitive visual detection and easy‐to‐use innovative technology for the detection of BmNPV. A 10‐fold dilution (2 μg–0.02 pg) of DNA extracted from BmNPV infected silkworms was used to determine the detection limit of the LAMP‐LFD assay as well as the PCR assay, which typically indicated 0.2 pg as the LOD for LAMP‐LFD which is 100 times more sensitive than the LOD for PCR which was observed to be 20 pg (Zhou et al., 2015).…”

Section: Molecular Tools For the Detection Of Silkworm Pathogensmentioning

confidence: 99%

Molecular diagnostics in sericulture: A paradigm shift towards disease diagnosis in silkworms

Deepika,

Ramesh,

Kumar

et al. 2024

Entomologia Exp Applicata

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Sericulture, the practice of rearing silkworms for the production of silk, is an essential agro‐based industry in several countries. However, silkworms are susceptible to a variety of diseases caused by viruses, bacteria and parasites, which may have a significant negative impact on global silk production. Traditional methods of pathogen identification, such as microscopy and laboratory culturing, have limitations in terms of accuracy and efficiency. The development of molecular techniques for pathogen identification has revolutionised the field of sericulture over the last decade. Genomic DNA and RNA‐based molecular techniques allow for the rapid and accurate detection of disease‐causing pathogens in silkworms. Molecular diagnosis has several advantages over traditional methods, including increased sensitivity and specificity, shorter turnaround time and the ability to detect pathogens that are difficult to culture or visualise under a microscope. Molecular techniques have been applied to detect several important pathogens of silkworms, including Nosema sp., nucleopolyhedrovirus, cypovirus, iflavirus and bidensovirus. The use of molecular diagnostics in sericulture is immensely important as the demand for high‐quality silk increases globally and the assessment of emerging pathogens associated with crop loss is essential. Major advancements in the improvement and application of molecular methods for diagnosing widespread silkworm pathogens are discussed.

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Section: Molecular Tools For the Detection Of Silkworm Pathogensmentioning

confidence: 99%