Rapid Detection of Ag(I) via Size-Induced Photoluminescence Quenching of Biocompatible Green-Emitting, <scp>l</scp>-Tryptophan-Scaffolded Copper Nanoclusters

Aarya, None; Thomas, Telna; Sarangi, Bibhu Ranjan; Mojumdar, Supratik Sen

doi:10.1021/acsomega.3c00462

Cited by 9 publications

(3 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lysozyme-protected copper nanocluster (Lys-Cu NC) and the tryptophan-capped copper nanocluster (Trp-Cu NC) were synthesized following a previously reported protocol. , …”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Temperature-Induced Luminescence Intensity Fluctuation of Protein-Protected Copper Nanoclusters: Role of Scaffold Conformation vs Nonradiative Transition

Sebastian,

Aarya

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

Protein-scaffolded atomically precise metal nanoclusters (NCs) have emerged as a promising class of biofriendly nanoprobes at the forefront of modern research, particularly in the area of sensing. The photoluminescence (PL) intensity of several nanoclusters showed a systematic temperature-dependent fluctuation, but the mechanism remains ambiguous and is poorly understood. We tried to shed some light on this mechanistic aspect by testing a couple of hypotheses: (i) conformational fluctuation of the protein scaffold-mediated PL intensity fluctuation and (ii) PL intensity fluctuation due to the variation in the radiative and nonradiative transition rates. Herein, the PL intensity of the lysozyme-capped copper nanocluster (Lys-Cu NC) showed excellent temperature dependency; upon increasing the temperature, the PL intensity gradually decreased. However, contrasting effects can be seen when the nanocluster is exposed to a chemical denaturant (guanidine hydrochloride (GdnHCl)); the PL intensity increased with the increase in the GdnHCl concentration due to the change in the ionic strength of the medium. This discrepancy clearly suggests that the thermal PL intensity fluctuation cannot be explained by a change in the scaffold conformation. Furthermore, upon closer investigation, we observed a 2-fold increase in the nonradiative decay rate of the Lys-Cu NC at the elevated temperature, which could reasonably explain the decrease in the PL intensity of the nanocluster at the higher temperature. Additionally, from the result, it was evident that the protein scaffold−metal core interaction played a key role here in stabilizing each other; hence, the scaffold structure remained unaffected even in the presence of chemical denaturants.

show abstract

“…The lysozyme-protected copper nanocluster (Lys-Cu NC) and the tryptophan-capped copper nanocluster (Trp-Cu NC) were synthesized following a previously reported protocol. , …”

Section: Methodsmentioning

confidence: 99%

“…To date, a wide variety of scaffolds such as proteins, , peptides, , DNA, , polymer, dendrimers, amino acids, − etc. have been extensively used for the facile synthesis of noble metal NCs.…”

Section: Introductionmentioning

confidence: 99%

Temperature-Induced Luminescence Intensity Fluctuation of Protein-Protected Copper Nanoclusters: Role of Scaffold Conformation vs Nonradiative Transition

Sebastian,

Aarya

et al. 2024

ACS Omega

Self Cite

View full text Add to dashboard Cite

show abstract

“…The solid line in Fig. 5c is the theoretical curve obtained according to this method (Aarya et al 2023).…”

Section: Quenching Mechanismmentioning

confidence: 99%

Synthesis, theoretical analysis, and biological properties of a novel tridentate Schiff base palladium (II) complex

Jahangiry,

Lashanizadegan,

Pourhosseini

et al. 2024

Biometals

View full text Add to dashboard Cite

Schiff base complexes play a crucial role in bioinorganic chemistry. A novel curcumin/phenylalanine tridentate Schiff base ligand and its palladium (II) complex were synthesized in order that they were stable in aqueous buffer. The structure of the complex was investigated using a variety of methods, including DFT, NBO analysis, FMOs, and MESP. The interaction of the complex with plasmid (pUC19) and CT-DNA was studied. The anticancer, antibacterial, and antioxidant activities of the complex were examined. The statistical analysis of MTT assay was compared using the 1-way ANOVA and Tukey test. Results showed that the complexes were stable in aqueous buffer, pH 8. The extrinsic fluorescence emission of the plasmid and CT-DNA was quenched while interacting with the complex. The complex had an IC50 of 72.47 μM against MCF-7 cells. The ANOVA and Tukey analysis of MTT data demonstrated a statistically significant difference between groups (P<0.0001). The minimum inhibitory concentrations (MIC) of the complex for E. coli and S. aureus were 300 and 200 μg/mL, with 96.3 % and 95.2 % biofilm growth inhibition at 250 μg/mL, respectively. The sample concentrations contributing to 50% radical inhibition in the 1,1-diphenyl-2picrylhydrazyl (DPPH) test for curcumin, ligand, and palladium (II) complex were 33.62, 21.27, and 51.26 μM, respectively. The results suggest that the complex interaction with DNA is one of the potential mechanisms in the elimination of cancer cells and bacteria in the planktonic and biofilm. On the other hand, while stability in aqueous buffer at pH 8 increases, modified curcumin antioxidant effect decreases.

show abstract

Role of a mild reducing agent in designing a copper nanocluster-based tunable dual-metal sensor for the selective and sensitive detection of Ag+ and Fe2+

Sebastian

Aarya

Kavya

et al. 2023

Chemical Physics Impact

View full text Add to dashboard Cite

Rapid Detection of Ag(I) via Size-Induced Photoluminescence Quenching of Biocompatible Green-Emitting, l-Tryptophan-Scaffolded Copper Nanoclusters

Cited by 9 publications

References 86 publications

Temperature-Induced Luminescence Intensity Fluctuation of Protein-Protected Copper Nanoclusters: Role of Scaffold Conformation vs Nonradiative Transition

Temperature-Induced Luminescence Intensity Fluctuation of Protein-Protected Copper Nanoclusters: Role of Scaffold Conformation vs Nonradiative Transition

Synthesis, theoretical analysis, and biological properties of a novel tridentate Schiff base palladium (II) complex

Role of a mild reducing agent in designing a copper nanocluster-based tunable dual-metal sensor for the selective and sensitive detection of Ag+ and Fe2+

Contact Info

Product

Resources

About