Rapid Detection Methods for Bacterial Pathogens in Ambient Waters at the Point of Sample Collection: A Brief Review

Li, Jing; Zhu, Yuqing; Wu, Xunyi; Hoffmann, Michael R.

doi:10.1093/cid/ciaa498

Cited by 41 publications

(29 citation statements)

References 50 publications

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“…Most of these analytes are of low abundance and will require a biosensor with high sensitivity. This is an example of the importance of identifying the biosensor target analyte, which is the major issue to resolve (38. The development of whole cell-based biosensors has rapidly accelerated, demonstrating their huge potential in biomedical diagnosis and analysis of environmental and food matrices (15,39,40). The major advantages of these systems are high sensitivity and selectivity, reagentless rapid detection, and cost-effective testing during routine inspections or at the points of care (25).…”

Section: Biosensors As Medical Devicesmentioning

confidence: 99%

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Barbosa

Cepeda

León-Torres

et al. 2021

Vitae

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BACKGROUND : Biosensing techniques have been the subject of exponentially increasing interest due to their performance advantages such as high selectivity and sensitivity, easy operation, low cost, short analysis time, simple sample preparation, and real-time detection. Biosensors have been developed by integrating the unique specificity of biological reactions and the high sensitivity of physical sensors. Therefore, there has been a broad scope of applications for biosensing techniques, and nowadays, they are ubiquitous in different areas of environmental, healthcare, and food safety. Biosensors have been used for environmental studies, detecting and quantifying pollutants in water, air, and soil. Biosensors also showed great potential for developing analytical tools with countless applications in diagnosing, preventing, and treating diseases, mainly by detecting biomarkers. Biosensors as a medical device can identify nucleic acids, proteins, peptides, metabolites, etc.; these analytes may be biomarkers associated with the disease status. Bacterial food contamination is considered a worldwide public health issue; biosensor-based analytical techniques can identify the presence or absence of pathogenic agents in food. OBJECTIVES: The present review aims to establish state-of-the-art, comprising the recent advances in the use of nucleic acid-based biosensors and their novel application for the detection of nucleic acids. Emphasis will be given to the performance characteristics, advantages, and challenges. Additionally, food safety applications of nucleic acid-based biosensors will be discussed. METHODS: Recent research articles related to nucleic acid-based biosensors, biosensors for detecting nucleic acids, biosensors and food safety, and biosensors in environmental monitoring were reviewed. Also, biosensing platforms associated with the clinical diagnosis and food industry were included. RESULTS: It is possible to appreciate that multiple applications of nucleic acid-based biosensors have been reported in the diagnosis, prevention, and treatment of diseases, as well as to identify foodborne pathogenic bacteria. The use of PNA and aptamers opens the possibility of developing new biometric tools with better analytical properties. CONCLUSIONS: Biosensors could be considered the most important tool for preventing, treating, and monitoring diseases that significantly impact human health. The aptamers have advantages as biorecognition elements due to the structural conformation, hybridization capacity, robustness, stability, and lower costs. It is necessary to implement biosensors in situ to identify analytes with high selectivity and lower detection limits.

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Section: Biosensors As Medical Devicesmentioning

confidence: 99%

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Barbosa

Cepeda

León-Torres

et al. 2021

Vitae

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“…A key factor in understanding and possibly managing the pathogenic component of environmental stressors is monitoring the presence of various pathogens, and especially those deemed to be opportunistic [ 16 ]. Monitoring the prevalence and extent of pathogenic outbreaks is easily performed by PCR using environmental samples [ 17 , 18 , 19 ]. However, this information may not reveal the potential for infectivity, transmission, or immunological responses over time and distance [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Quantifying Circulating IgY Antibody Responses against Select Opportunistic Bacterial Pathogens and Correlations with Body Condition Factors in Wild American Alligators, Alligator mississippiensis

Alston

Rainwater

Parrott

et al. 2022

Biology

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Little is known about the disease ecology of American alligators (Alligator mississippiensis), and especially how they respond immunologically to emerging infectious diseases and zoonotic pathogens. In this study, we examined serum samples collected from wild alligators in Florida (2010–2011) and South Carolina (2011–2012, 2014–2017) for antibody responses to multiple bacteria. Immunoglobulin Y (IgY) was purified from serum to generate a mouse monoclonal antibody (mAb AMY-9) specific to the IgY heavy chain. An indirect ELISA was then developed for quantifying antibody responses against whole cell Escherichia coli, Vibrio parahaemolyticus, Vibrio vulnificus, Mycobacterium fortuitum, Erysipelothrix rhusiopthiae, and Streptococcus agalactiae. In Florida samples the primary differences in antibody levels were between January–March and late spring through summer and early fall (May–October), most likely reflecting seasonal influences in immune responses. Of note, differences over the months in antibody responses were confined to M. fortuitum, E. rhusiopthiae, V. vulnificus, and E. coli. Robust antibody responses in SC samples were observed in 2011, 2014, and 2015 against each bacterium except E. coli. All antibody responses were low in 2016 and 2017. Some of the highest antibody responses were against V. parahaemolyticus, M. fortuitum, and E. rhusiopthiae. One SC alligator estimated to be 70+ years old exhibited the highest measured antibody response against V. parahaemolyticus and M. fortuitum. By combining data from both sites, we show a clear correlation between body-mass-indices (BMI) and antibody titers in all six of the bacteria examined. Our study provides a critical antibody reagent and a proof-of-concept approach for studying the disease ecology of alligators in both the wild and in captivity.

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“… 20 , 21 To address these challenges, isothermal NAA methods, such as loop-mediated isothermal amplification (LAMP), are used for environmental quantification of other microbial pathogens including Zika virus, 22 astrovirus, 23 MS2, 24 Escherichia coli , and Enterococcus spp. 25 , 26 RT-LAMP quantification has higher tolerances to inhibitors and shorter amplification times (e.g., 30 min) compared to RT-qPCR. 24 , 27 , 28 Huang et al used a portable in-gel LAMP platform for the sensitive detection of MS2 coliphage in wastewater, while RT-qPCR failed to produce a positive result.…”

Section: Introductionmentioning

confidence: 99%

Membrane-Based In-Gel Loop-Mediated Isothermal Amplification (mgLAMP) System for SARS-CoV-2 Quantification in Environmental Waters

Zhu

et al. 2021

Environ. Sci. Technol.

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Since the COVID-19 pandemic is expected to become endemic, quantification of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in ambient waters is critical for environmental surveillance and for early detection of outbreaks. Herein, we report the development of a membrane-based in-gel loop-mediated isothermal amplification (mgLAMP) system that is designed for the rapid point-of-use quantification of SARS-CoV-2 particles in environmental waters. The mgLAMP system integrates the viral concentration, in-assay viral lysis, and on-membrane hydrogel-based RT-LAMP quantification using enhanced fluorescence detection with a target-specific probe. With a sample-to-result time of less than 1 h, mgLAMP successfully detected SARS-CoV-2 below 0.96 copies/mL in Milli-Q water. In surface water, the lowest detected SARS-CoV-2 concentration was 93 copies/mL for mgLAMP, while the reverse transcription quantitative polymerase chain reaction (RT-qPCR) with optimal pretreatment was inhibited at 930 copies/mL. A 3D-printed portable device is designed to integrate heated incubation and fluorescence illumination for the simultaneous analysis of nine mgLAMP assays. Smartphone-based imaging and machine learning-based image processing are used for the interpretation of results. In this report, we demonstrate that mgLAMP is a promising method for large-scale environmental surveillance of SARS-CoV-2 without the need for specialized equipment, highly trained personnel, and labor-intensive procedures.

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Rapid Detection Methods for Bacterial Pathogens in Ambient Waters at the Point of Sample Collection: A Brief Review

Cited by 41 publications

References 50 publications

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Nucleic acid-based biosensors: analytical devices for prevention, diagnosis and treatment of diseases

Quantifying Circulating IgY Antibody Responses against Select Opportunistic Bacterial Pathogens and Correlations with Body Condition Factors in Wild American Alligators, Alligator mississippiensis

Membrane-Based In-Gel Loop-Mediated Isothermal Amplification (mgLAMP) System for SARS-CoV-2 Quantification in Environmental Waters

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