Rapid detection and identification of two lineages of influenza B strains with monoclonal antibodies

Human RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus in Canine Cells

“…To amplify the rescued virus more efficiently, fresh feeder MDCK 33016-PF cells in serum-free media plus 0.5 g/ml TrypZean (Sigma) were added. Cleared medium supernatants were collected at 72 h posttransfection, and virus titers were determined on fresh MDCK 33016-PF cells using a slight modification of previously described focus-forming assays (10,12). Using this optimized virus rescue protocol, we were able to rescue the A/Puerto Rico/8/34 virus in each cell type.…”

Emergence of an Influenza B Virus with Antigenic Change

“…MAbs were obtained by immunizing mice with influenza B virus strains as described previously (9)(10)(11)(12). Ascitic fluid samples from mice injected with hybridoma cells were used as the sources of MAbs.…”

“…The standard ferret sera were provided by the National Institute of Health, Tokyo, Japan: the sera against influenza virus B/Yamanashi/166/98 for influenza virus B/Yamagata and the sera against influenza virus B/Shangdong/ 7/97 for influenza virus B/Victoria. The methods for virus inoculation and visualization of infected cells by peroxidaseantiperoxidase (PAP) staining were described previously (9,14,16). Briefly, infected cells were treated successively with MAbs, rabbit anti-mouse immunoglobulin antibody, goat antirabbit immunoglobulin antibody, and PAP complex.…”

Discovery of the Neutralizing Epitope Common to Influenza B Virus Victoria Group Isolates in Japan

“…Site B and site A (a protruding loop region) of the HA molecule have been reported to be the highly immunodominant regions of influenza A and B viruses (1,17). We developed monoclonal antibodies (MAbs) 10B8 and 10D7, whose epitopes had been conserved at site B of B/Victoria isolates since the mid-1980s until the 1996/1997 season (7,9). However, the 2002/2003 isolates in Kobe, Japan, were divided into three groups according to their reactivities to 10B8 and 10D7.…”