Rapid detection and identification methods for Listeria monocytogenes in the food chain – A review

Välimaa, Anna-Liisa; Tilsala-Timisjärvi, Anu; Virtanen, Elina

doi:10.1016/j.foodcont.2015.02.037

Cited by 157 publications

(99 citation statements)

References 102 publications

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“…3 However, the two target genes were not specific to L. monocytogenes, iap gene was present in all six Listeria species and hlyA gene for Listeria seeligeri, L. ivanovii, and L. monocytogenes. 15 In this study, the MCDA primer sequences were based on the species-specific gene (lmo0733), which was unique to L. monocytogenes.…”

Section: Discussionmentioning

confidence: 97%

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of <em>Listeria monocytogenes</em>

Wang¹,

Li²,

Wang³

et al. 2017

IJN

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Section: Discussionmentioning

confidence: 97%

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of <em>Listeria monocytogenes</em>

Wang¹,

Li²,

Wang³

et al. 2017

IJN

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“…The invA marker contains a highly conserved sequence unique for Salmonella DNA and its amplification is considered the international gold standard for detection of this pathogen (9). Several virulence-associated markers have been proposed for L. monocytogenes identification, but the hly gene, which encodes listeriolysin O, is one of the most frequently used (17,29). Regarding VTEC, the virulence markers stx1, stx2, and eae targeted in the present study encode Shiga toxins and adhesion protein intimin respectively (2).…”

Section: Discussionmentioning

confidence: 99%

Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals

Denis

Bielińska

Wieczorek

et al. 2016

Journal of Veterinary Research

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Introduction: The study objective was to develop and evaluate a new TaqMan multiplex real-time PCR method for Salmonella, L. monocytogenes, and verotoxigenic Escherichia coli (VTEC) detection in slaughtered animal carcasses. Material and Methods: The procedure included an enrichment step, DNA extraction, and two multiplex real-time PCRs. The first PCR detected the invA and hly genes of Salmonella and L. monocytogenes respectively, the second the vtx1, vtx2, and eae genes of VTEC. Results: The validation of this method resulted in 100% relative sensitivity, specificity, and accuracy as compared to the reference ISO methods. The limit of detection per swab sample was established at 1 cfu for Salmonella and L. monocytogenes and 2 cfu for VTEC. The authors analysed 265 slaughterhouse-collected swabs from cattle, pig, and poultry carcasses. Among 125 from cattle, 51 were positive for VTEC, 29 for Salmonella, and 1 for L. monocytogenes. Among swabs from pig carcasses (n = 95), three, two, and one sample were positive for these pathogens respectively. None of the microorganisms tested for was identified in 45 samples of poultry origin. Conclusion: The obtained results showed that the method developed can rapidly identify the main bacterial pathogens that may contaminate carcasses of food-producing animals.

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“…This microorganism represents the ability to grow under aerobic and anaerobic conditions [68], at a wide range of temperature ( 0.4°C to 45°C) [69,70], over a wide pH range from 4.0 to 9.6 [71], in a modified atmosphere packaging [72], and even at relatively low water activity levels [73]. These features make Listeria a serious concern in food industry that requires strong controls along the food chain [74].…”

Section: Pressure Of 35 Mpa and Temperature Of 36°cmentioning

confidence: 99%

Recovery of valuable components and inactivating microorganisms in the agro-food industry with ultrasound-assisted supercritical fluid technology

Koubàa

Mhemdi

Fages

2018

The Journal of Supercritical Fluids

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Food industry is increasingly interested in replacing conventional processes of plant extraction, as well as microbial inactivation, by alternative, greener and cheaper techniques. In many cases, chemical extraction processes are hindered by several drawbacks such as the use of chemical solvents, which is associated with many health and environmental issues. Furthermore, the use of high temperatures to improve and/or accelerate the processes, which may affect and degrade the thermolabile compounds, is usually required. Among the most promising techniques that could be competitive to the current extraction methods of molecules from plant matrices and microbial inactivation of food products, ultrasound assisted supercritical fluid extraction has taken great interest. This non-exhaustive review, covering the last 20 years, gives a critical commentary on the main published results using this emerging technology. Promising prospects opened up by new applications are described, without omitting the main limitations still to be overcome for a harmonious industrial development of these technologies.

show abstract

Rapid detection and identification methods for Listeria monocytogenes in the food chain – A review

Cited by 157 publications

References 102 publications

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of <em>Listeria monocytogenes</em>

Development of multiple cross displacement amplification label-based gold nanoparticles lateral flow biosensor for detection of <em>Listeria monocytogenes</em>

Multiplex real-time PCRs for detection of Salmonella, Listeria monocytogenes, and verotoxigenic Escherichia coli in carcasses of slaughtered animals

Recovery of valuable components and inactivating microorganisms in the agro-food industry with ultrasound-assisted supercritical fluid technology

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