Rapid, colorimetric quantification of lipid from algal cultures

Wawrik, Boris; Harriman, Brian H.

doi:10.1016/j.mimet.2010.01.016

Cited by 61 publications

(42 citation statements)

References 12 publications

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“…Glycogen concentration was estimated relatively to calibration curve prepared using glucose concentrations of 2-20 lg/ml. Total lipid concentration was determined according to the procedure of Wawrik and Harriman (2010) with some modifications. Flies were homogenized in a ratio 1:20 (w:v) in 25% ethanol solution with 1.5 M NaOH at temperature of 21°C.…”

Section: Determination Of Glycogen Total Lipid and Protein Concentramentioning

confidence: 99%

High sucrose consumption promotes obesity whereas its low consumption induces oxidative stress in Drosophila melanogaster

Rovenko

Kubrak

Gospodaryov

et al. 2015

Journal of Insect Physiology

101

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Section: Determination Of Glycogen Total Lipid and Protein Concentramentioning

confidence: 99%

High sucrose consumption promotes obesity whereas its low consumption induces oxidative stress in Drosophila melanogaster

Rovenko

Kubrak

Gospodaryov

et al. 2015

Journal of Insect Physiology

101

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“…TD-NMR is a fast and convenient method to quantify lipid of C. protothecoides (Gao et al 2008), and its application for other microalgal species needs further investigation. Wawrik and Harriman (2010) developed a simple colorimetric method for quantification of algal lipid from small cultures. Algal lipids are saponified into fatty acids and reacted with a copper reagent.…”

Section: Determination Of Microalgal Lipid Contentmentioning

confidence: 99%

Biodiesel production with microalgae as feedstock: from strains to biodiesel

2011

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Due to negative environmental influence and limited availability, petroleum-derived fuels need to be replaced by renewable biofuels. Biodiesel has attracted intensive attention as an important biofuel. Microalgae have numerous advantages for biodiesel production over many terrestrial plants. There are a series of consecutive processes for biodiesel production with microalgae as feedstock, including selection of adequate microalgal strains, mass culture, cell harvesting, oil extraction and transesterification. To reduce the overall production cost, technology development and process optimization are necessary. Genetic engineering also plays an important role in manipulating lipid biosynthesis in microalgae. Many approaches, such as sequestering carbon dioxide from industrial plants for the carbon source, using wastewater for the nutrient supply, and maximizing the values of by-products, have shown a potential for cost reduction. This review provides a brief overview of the process of biodiesel production with microalgae as feedstock. The methods associated with this process (e.g. lipid determination, mass culture, oil extraction) are also compared and discussed.

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“…Chlorella microalgae have been present on earth since the preCambrian period-2.5 billion years ago. Japan is currently the world leader in Chlorella microalgae consumption [1][2][3]. The name Spirulina was based on its spiral shaped.…”

Section: Introductionmentioning

confidence: 99%

Chlorella and Spirulina Microalgae as Sources of Functional Foods, Nutraceuticals, and Food Supplements; an Overview

Andrade

Dias

et al. 2018

MOJFPT

192

115

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Chlorella and Spirulina are the two of the most well-known microalgae genus. Both microalgae genus have a significant content of proteins, vitamins, pigments, fatty acids, sterols, among others, which make their production/application by the food industry quite interesting. Chlorella genus is a eukaryotic microorganism, whereas Spirulina genus (cyanobacteria) is a prokaryotic microorganism. The aim of this review was to provide an overview on Chlorella and Spirulina microalgae, particularly as an alternative source of functional foods, nutraceuticals, and food supplements, in which the following compound groups were addressed: (I) Long-Chain Polyunsaturated Fatty Acids; (II) Phenolic Compounds; (III) Volatile Compounds; (IV) Sterols; (V) Proteins, Amino Acids, Peptides; (VI) Vitamins; (VII) Polysaccharides; (VIII) Pigments and (IX) Food. Chlorella and Spirulina microalgae and their derivatives are concluded not to be widely commercially exploited. However, they are remarkable sources of functional foods, nutraceuticals and food supplements.

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Rapid, colorimetric quantification of lipid from algal cultures

Cited by 61 publications

References 12 publications

High sucrose consumption promotes obesity whereas its low consumption induces oxidative stress in Drosophila melanogaster

High sucrose consumption promotes obesity whereas its low consumption induces oxidative stress in Drosophila melanogaster

Biodiesel production with microalgae as feedstock: from strains to biodiesel

Chlorella and Spirulina Microalgae as Sources of Functional Foods, Nutraceuticals, and Food Supplements; an Overview

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