Rapid colorimetric assay of β-galactosidase and N-acetyl-β-glucosaminidase in human urine

Maruhn, D

doi:10.1016/0009-8981(76)90147-9

Cited by 319 publications

(104 citation statements)

References 8 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…NAG is a high molecular weight lysosomal enzyme present in renal tubular cells (1,31). The excretion rate is not affected by serum concentrations since NAG is not filtered by the glomeruli.…”

Section: Discussionmentioning

confidence: 99%

Biochemical profile of amniotic fluid for the assessment of fetal and renal development

Oliveira

Barros

Magalhães

2002

Braz J Med Biol Res

View full text Add to dashboard Cite

Creatinine plays a key role in the function and maturation of fetal kidneys throughout pregnancy. It is important to identify other markers that may help in the diagnosis of renal dysfunction. Our aim was to determine the profile of and the correlation between biochemical markers to be used to assess renal function and maturation of the fetus in the amniotic fluid during pregnancy and to determine the distribution of normal values for creatinine, N-acetyl-ß-D-glucosaminidase (NAG), ß2-microglobulin, glucose, urea, sodium, potassium, phosphorus, calcium, uric acid, albumin, and osmolality in three gestational age groups. This was a cross-section study that assessed 115 samples of amniotic fluid during three different periods of pregnancy, i.e., 13 to 20, 27 to 34, and 36 to 42 weeks. Concentrations of creatinine, NAG, urea, potassium and uric acid increased during pregnancy (P<0.05). ß2-Microglobulin, glucose, sodium, phosphorus, calcium, and albumin concentration and osmolality decreased (P<0.05), whereas ß2-microglobulin, glucose and uric acid presented significant correlations with gestational age and creatinine, respectively (r>0.6, P<0.05). Urea, potassium and phosphorus showed mild correlations with both (r>0.5, P<0.05). NAG, sodium, albumin and osmolality did not show significant correlations (r<0.5, P<0.05). These tests confirmed the important role of creatinine in terms of correlation with gestational age. ß2-Microglobulin, glucose and uric acid were significant as markers of function and maturation of fetal kidneys, whereas NAG did not demonstrate a useful role for the assessment of renal maturation.

show abstract

Section: Discussionmentioning

confidence: 99%

Biochemical profile of amniotic fluid for the assessment of fetal and renal development

Oliveira

Barros

Magalhães

2002

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…N-acetyl glucosaminidase activity was assessed by the method of Maruhn 23 and the activity of b-galactosidase was assessed by the method of Rosenblit et al 24 Cathepsin D activity was calculated by the method of Biber et al 25 using hemoglobin as the substrate and the activity expressed as mmol of tyrosine liberated/h/mg protein.…”

Section: Assay Of Lysosomal Enzymes and Glycoproteinsmentioning

confidence: 99%

Protective effect of administration ofWithania somiferaagainst bromobenzene induced nephrotoxicity and mitochondrial oxidative stress in rats

2014

View full text Add to dashboard Cite

Background: The present study was conducted to elucidate the protective role of Withania somnifera against bromobenzene induced nephrotoxicity and mitochondrial dysfunction in rats. Methods: In this study, Wistar albino rats of either sex were divided into six groups consisting of six animals each. The first one was control, those in group II received bromobenzene (10 mmol/kg, intragastric intubation) once, but group III and IV animals received W. somnifera (250 and 500 mg/kg, orally), respectively for 8 days followed by bromobenzene once on the 8th day and silymarin (100 mg/kg, orally) was given for 8 days to group V animals and then bromobenzene on the last day. Group VI animals received only W. somnifera (500 mg/kg) for 8 days. Results: The levels of renal lipid peroxidation, lysosomal enzymes and glycoprotein were increased significantly (p50.05) in the bromobenzene alone treated rats and antioxidant status and mitochondrial enzymes were found to be decreased, when compared to the control group. The levels of kidney functional markers (urea, uric acid and creatinine) were also found to be abnormal in serum of bromobenzene alone treated rats. On the other hand, administration of W. somnifera (250 and 500 mg/kg) along with bromobenzene offered a significant dose-dependent protection to the biochemical alterations as observed in the bromobenzene alone treated rats, which was also evidenced by histopathology. Conclusion: Thus, the oral administration of W. somnifera significantly protected against the bromobenzene induced nephrotoxicity and renal mitochondrial dysfunction in rats.

show abstract

“…The calculation of NAG was carried out according to the modified Maruch method. [4] According to this method, NAG activity is proportional to the amount of n-nitrophenol, which is formed as a result of the hydrolytic reaction of n-nitro-N-acetyl-β-glucosamine catalyzed by this enzyme. The measurement of the n-nytrophenol amount was carried out by spectrophotometry at λ=400 nm.…”

Section: Methodsmentioning

confidence: 99%

Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis

Zharikov¹,

Saldan²,

Киселев³

et al. 2017

Int J Biomed

View full text Add to dashboard Cite

The aim of our study was to test anti-lithogenic activity of the new biomedical substance from pig kidneys (PK-BMS) on the experimentally induced nephrolithiasis. Materials and Methods:The experiments were performed with 30 male Wistar outbred rats weighting 220-280g. The experimental animals were divided into two groups. In the disease-control group (DCG), the animals received a 1% solution of EG for 6 weeks. The rats in therapy group (TG), beginning from the fourth week, in addition to the daily EG intake, received PK-BMS in the amount of 1.0 per rat. The production of this substance was carried out at Altaivitaminy ZAO (Biysk, Altai Krai, Russia) by the method of cool dehumidification. In daily urine, we determined the concentration of oxalate, phosphate and calcium ions, creatinine excretion every 3 or 4 days in both groups; the activity of LDH, GGT, and NAG was measured every 7 days in the injured kidney epithelium. For the study of free-radical oxidation activity and for morphological examination of animal kidneys, after 6 weeks of the experiment, 5 rats from each group were euthanized. The occurrence of calcium compounds was determined by von Kossa's staining method.Results: After a 6-week EG intake, we found the typical biochemical and morphological symptoms of experimental nephrolithiasis in rats of DCG: urine supersaturation with oxalate ions, significant increase in the activity of marker enzymes, activation of free-radical oxidation in the kidneys, and formation of calcium deposits in the kidneys. In TG, the new PK-BMS intake resulted in a significant alleviation of the experimental nephrolithiasi: significant decrease in the level of oxalate ions and activity of marker enzymes, reduction of free-radical oxidation in the kidneys, decrease in the number and size of calcium deposits in the area of renal papillae.Conclusion: It was established that during the experimental nephrolithiasis, a three-week intake of the new PK-BMS is accompanied by a significant anti-lithogenic effect. (Int J Biomed. 2017; 7(1):41-45.)

show abstract

Rapid colorimetric assay of β-galactosidase and N-acetyl-β-glucosaminidase in human urine

Cited by 319 publications

References 8 publications

Biochemical profile of amniotic fluid for the assessment of fetal and renal development

Biochemical profile of amniotic fluid for the assessment of fetal and renal development

Protective effect of administration ofWithania somiferaagainst bromobenzene induced nephrotoxicity and mitochondrial oxidative stress in rats

Experimental Testing of the New Biomedical Substance of Pig Kidneys for the Treatment of Nephrolithiasis

Contact Info

Product

Resources

About