Rapid capture of biomolecules from blood <i>via</i> stimuli-responsive elastomeric particles for acoustofluidic separation

Li, Linying; Shields, C. Wyatt; Huang, Jin; Zhang, Yiqun; Ohiri, Korine A.; Yellen, Benjamin B.; Chilkoti, Ashutosh; López, Gabriel P.

doi:10.1039/d0an01164a

Cited by 6 publications

(6 citation statements)

References 67 publications

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“…Reproduced with permission. [ 70 ] Copyright 2020, Royal Society of Chemistry. C) The left: the silicon wafer was used to etch the microfluidic chip, which consisted of three layers: borosilicate glass, silicon, and a PZT piezoelectric transducer.…”

Section: Different Designs In Acoustofluidic Devices For Micro/nanopa...mentioning

confidence: 99%

“…Aside from particle separation, BAW-based microfluidics have been investigated for use in biomarker isolation. For example, Li et al [70] created a BAW-based separation device, using the bulk standing acoustic waves (BSAWs) generated from a PZT piezoelectric transducer with a 2.93 MHz resonance frequency to isolate the desired protein-microparticle complexes from blood cells at a flow rate of 3 mL h −1 . And they took advantage of a linking agent, a form of thermally responsive polypeptide connected to ligands, that specifically captures proteins into silicone microparticles (Figure 3B).…”

Section: The Various Configurations In Baw Microfluidicsmentioning

confidence: 99%

“…Finally, the proteins were able to be released from the silicone particles by cooling the solution below a certain temperature. Reproduced with permission [70]. Copyright 2020, Royal Society of Chemistry.…”

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confidence: 99%

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Acoustofluidics: A Versatile Tool for Micro/Nano Separation at the Cellular, Subcellular, and Biomolecular Levels

Zhao

Yang

et al. 2023

Adv Materials Technologies

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Separation of micro/nanoparticles, such as cellular, subcellular and biomolecular, has attracted increasing attention because of their remarkable potential applications in various fields, including chemistry, physics, medicine, etc. Among different micro/nanoparticle separation methods, acoustofluidics, which combines acoustics and microfluidics, has drawn the interest of researchers due to its biocompatibility, high efficiency and free labeling. In this review, the basic constitutions, mechanisms, and materials of acoustofluidics are described. Subsequently, sorts of delicately designed acoustofluidic devices, including diverse bulk acoustic wave (BAW) microfluidics and surface acoustic wave (SAW) microfluidics, are discussed, covering principles, advantages, limitations and applications in separation. Besides the introduction of advances of micro/nanoparticle separation in the BAW microfluidics, the SAW microfluidics are elaborated in detail with a focus on various configurations of interdigital transducers (IDTs), comprising straight IDT, slanted‐finger IDT, chirped IDT and focused IDT. Microfluidic systems of the acoustofluidics involve the forms of straight channels, serpentine channels, and droplets. Additionally, besides simply structured acoustofluidics, acoustofluidics integrated with other structures are also mentioned. Finally, the prospects and limitations of acoustofluidics in micro/nanoparticle separation are also discussed. The acoustofluidics reviewed here is envisioned as a versatile tool for micro/nanoparticle separation at the cellular, sub‐cellular, and biomolecular levels.

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Section: Different Designs In Acoustofluidic Devices For Micro/nanopa...mentioning

confidence: 99%

Section: The Various Configurations In Baw Microfluidicsmentioning

confidence: 99%

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Acoustofluidics: A Versatile Tool for Micro/Nano Separation at the Cellular, Subcellular, and Biomolecular Levels

Zhao

Yang

et al. 2023

Adv Materials Technologies

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“…For instance, Ahmad et al successfully separated thrombin from mCardinal2 and human serum samples by capturing the proteins using aptamer-functionalized PS beads in a TSAW device 97 . Similarly, Li et al developed a type of thermally responsive polypeptide fused to ligands that acts as a linking agent to selectively capture proteins into silicone microparticles for separating specific proteins from blood using a BAW device 98 . In particular, the separated proteins could be released from the microparticles for downstream analysis by cooling the solution below the solubility temperature of the polypeptides.…”

Section: Biological Applications Of Acoustofluidic Separation Technol...mentioning

confidence: 99%

Recent advances in acoustofluidic separation technology in biology

et al. 2022

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Acoustofluidic separation of cells and particles is an emerging technology that integrates acoustics and microfluidics. In the last decade, this technology has attracted significant attention due to its biocompatible, contactless, and label-free nature. It has been widely validated in the separation of cells and submicron bioparticles and shows great potential in different biological and biomedical applications. This review first introduces the theories and mechanisms of acoustofluidic separation. Then, various applications of this technology in the separation of biological particles such as cells, viruses, biomolecules, and exosomes are summarized. Finally, we discuss the challenges and future prospects of this field.

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“…ELPs are based on sequence motifs from human tropoelastin, 21 characterized by the pentapeptide motif E X = VPGXG, where X is any amino acid except proline, and have been well-characterized. 22 Indeed, ELPs have found applications in biosensing, 23 tissue engineering, 24 nanoparticle coatings, 25 drug-delivery systems, 26 biomineralization studies, 27 immunoassays, 28 and molecular switches. 29 To obtain inert and hydrophilic polypeptide brushes, we choose serine as a guest residue in the ELPs, X = S. Some of us have previously developed a diblock polypeptide C−B K12 consisting of an oligolysine block B K12 = K 12 and a random coil hydrophilic block with a collagen-like sequence, C = (GXaaYaa) 132 .…”

Section: ■ Introductionmentioning

confidence: 99%

Self-Assembly of Elastin-like Polypeptide Brushes on Silica Surfaces and Nanoparticles

et al. 2021

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Control over the placement and activity of biomolecules on solid surfaces is a key challenge in bionanotechnology. While covalent approaches excel in performance, physical attachment approaches excel in ease of processing, which is equally important in many applications. We show how the precision of recombinant protein engineering can be harnessed to design and produce protein-based diblock polymers with a silica-binding and highly hydrophilic elastin-like domain that self-assembles on silica surfaces and nanoparticles to form stable polypeptide brushes that can be used as a scaffold for later biofunctionalization. From atomic force microscopy-based single-molecule force spectroscopy, we find that individual silica-binding peptides have high unbinding rates. Nevertheless, from quartz crystal microbalance measurements, we find that the self-assembled polypeptide brushes cannot easily be rinsed off. From atomic force microscopy imaging and bulk dynamic light scattering, we find that the binding to silica induces fibrillar self-assembly of the peptides. Hence, we conclude that the unexpected stability of these self-assembled polypeptide brushes is at least in part due to peptide–peptide interactions of the silica-binding blocks at the silica surface.

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Rapid capture of biomolecules from blood via stimuli-responsive elastomeric particles for acoustofluidic separation

Abstract: Detection of biomarkers often requires extensive sample preparation. We report a simple acoustofluidic method to isolate biomarkers on elastomeric particles by co-aggregation of elastin-like polypeptides, enabling their efficient capture and release.

Cited by 6 publications

References 67 publications

Acoustofluidics: A Versatile Tool for Micro/Nano Separation at the Cellular, Subcellular, and Biomolecular Levels

Acoustofluidics: A Versatile Tool for Micro/Nano Separation at the Cellular, Subcellular, and Biomolecular Levels

Recent advances in acoustofluidic separation technology in biology

Self-Assembly of Elastin-like Polypeptide Brushes on Silica Surfaces and Nanoparticles

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