Rapid Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Bacteria with Microwave Irradiation

Abstract

The development of rapid and reliable processes for the synthesis of nanosized materials is of great importance in the field of nanotechnology. Synthesis of silver nanoparticles using microorganism have been reported, but the process is rather slow. In this paper, we describe a novel combinatorial synthesis approach which is rapid, simple and “green” for the synthesis of metallic nanostructures of noble metals such as silver (Ag), by using a combination of culture supernatanant ofBacillus subtilisand microwave (MW) irradiation in water in absence of a surfactant or soft template. It was found that exposure of culture supernatanant ofBacillus subtilisand microwave irradiation to silver ion lead to the formation of silver nanoparticles. The silver nanoparticles were in the range of 5-60 nm in dimension. The nanoparticles were examined using UV-Visible Spectroscopy, and Transmission Electron Microscopy (TEM) analyses. The formation of nanoparticles by this method is extremely rapid, requires no toxic chemicals and the nanoparticles are stable for several months. The main conclusion is that the bio-reduction method to produce nanoparticles is a good alternative to the electrochemical methods.

Green synthesis of silver nanoparticles using Azadirachta indica leaf extract and its antimicrobial study

“…Green synthesis is preferred over conventional synthesis because it is ecofriendly, cost-effective, single-step method that can be easily scaled up for large scale synthesis and does not require high pressure, temperature, energy and toxic chemicals (Saha et al 2017). Many researchers have reported the use of materials such as plant leaf extract, root, stem, bark, leaf, fruit, bud and latex (Mariselvam et al 2014), fungi (Bhainsa 2006), bacteria (Saifuddin et al 2009) and enzymes (Willner et al 2007) for the synthesis of silver nano-particles. A lot of work has been done on green synthesis of silver nano-particles using microorganisms including bacteria, fungi and plants because of their antioxidant properties capable of reducing metal compounds in their respective nanoparticle.…”

Biogenic synthesis of zinc oxide nanoparticles using Ruta graveolens (L.) and their antibacterial and antioxidant activities

“…It is believed, that broad usage of these metal oxide nanoparticles may lead to their potentially hazardous the environment which could adversely affect human health. Their possible toxicity could induce cell membrane leakage, warranting the need to develop alternative methods of environmentally benign synthesis route (Limbach et al 2007) An eco-friendly, concerned materials like plant extract (Parashar et al 2009), bacteria (Saifuddin and Wong 2009), fungi (Bhainsa and Sauza 2006) and enzymes (Willner et al 2007) for the synthesis of silver nanoparticles offers numerous benefits of eco-friendly and compatibility for biomedical applications as they do not use toxic chemicals for the synthesis. Plant materials provide a biological synthesis route of several metallic nanoparticles which are more eco-friendly and allows a controlled synthesis with well-defined size and shape (Bar et al 2009).…”

Effect of Biosynthesized Silver Nanoparticles on Staphylococcus aureus Biofilm Quenching and Prevention of Biofilm Formation

“…Biological methods are regarded as safe, cost-effective, sustainable and environment friendly processes for the synthesis of nanoparticles [2]. Silver nanoparticles have been successfully synthesized using various bacteria [3][4][5], fungi [6,7] and plants [8,9].…”