Rapid Assembly of Heterogeneous 3D Cell Microenvironments in a Microgel Array

Li, Yiwei; Chen, Pu; Wang, Yachao; Yan, Shuangqian; Feng, Xiaojun; Du, Wei; Koehler, Stephan A.; Demirci, Utkan; Liu, Bi‐Feng

doi:10.1002/adma.201600247

Cited by 97 publications

(69 citation statements)

References 44 publications

(47 reference statements)

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“…"Worm-chip" have successfully used in phenotyping and stage screening, nerve system imaging, behavioral dynamics, microsurgery and microinjection [18][19][20][21][22][23][24][25][26][27]. Currently, several dilution micro-devices have been proposed for generating logarithmic, liner, and parabolic chemical concentration gradients [28][29][30][31][32][33][34][35][36]. Compared to traditional methods, microfluidic devices could generate linear chemical concentration gradient which shown great potential in studying chemotaxis behavior [22,[37][38][39], screening of chemotaxis-defective mutants [23,40] and drug screening in C. elegans [20,37,41].…”

Section: Introductionmentioning

confidence: 99%

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Wang

et al. 2018

Sensors and Actuators B: Chemical

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a b s t r a c t C. elegans is a commonly used model organism for studying the basic mechanisms of lifespan extension by dietary restriction (DR). Progress has been limited, however, by the lack of an automated system for quantitative analysis of this effect of bacterial diet. In this work, multifunctional logarithmic gradientcustomizing microfluidic device, which takes advantage of hydrodynamics, was developed to long-term maintain worm culture on the chip with parallel live imaging and generate logarithmic concentration gradient of bacteria. We successfully employed this platform to investigate the longevity and stress response of the worms in response to various concentrations of bacterial food. The results indicated that the lifespan extension of the worms was induced by DR when bacteria density was 10 8 cells mLand further dilutions would reduce the lifespan, which proposed a new dietary restriction regime in C. elegans. In addition, imaging analysis showed that DR regulated the subcellular localization DAF-16 in a way different from starvation that reduced lifespan of the worms. The platform allows not only horizontal studies of C. elegans in response to various environments over a wide range of concentrations, but also multiparameter evaluation of longevity in the worms with high temporal resolution, providing clues to better understand the aging process and help age-related drug screen.

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Section: Introductionmentioning

confidence: 99%

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Wang

et al. 2018

Sensors and Actuators B: Chemical

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“…Typically, high-throughput screening platforms consist of hydrogel microarrays with different material compositions, which enable the simultaneous analysis of a large number of interactions between cells and hydrogels. To this end, various techniques including contact printing, soft-lithography, and wettability patterning have been used to manufacture hydrogel microarrays [336–343]. …”

Section: Future Perspectivesmentioning

confidence: 99%

“…Briefly, nanoliter volumes of biomaterials could be inserted into the tips of pin arrays via the capillary force and transferred via contact between the tips and a substrate [344–346]. Combinatorial screenings of 2D and 3D cellular behaviors on biomaterial microarrays have been conducted by utilizing contact printing technique [336, 339, 344–350]. For instance, Dolatshahi-Pirouz et al utilized the contact printing method to print 3D cell-laden GelMA hydrogel arrays for the investigation of osteogenic differentiation of hMSCs [339].…”

Section: Future Perspectivesmentioning

confidence: 99%

“…Wettability patterning can potentially be used for screening 3D cellular behaviors in spatially engineered hydrogels. Recently, Li et al developed a simple method to generate spatially engineered 3D cell microenvironments using a surface-wettability-guided assembly (SWGA) method [336]. To make wettability patterns, a thin PDMS layer with microarray patterns was transferred to a clean glass substrate by using soft lithography.…”

Section: Future Perspectivesmentioning

confidence: 99%

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Spatially and temporally controlled hydrogels for tissue engineering

Leijten

Seo

Yue

et al. 2017

Materials Science and Engineering: R: Reports

156

129

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Summary Recent years have seen tremendous advances in the field of hydrogel-based biomaterials. One of the most prominent revolutions in this field has been the integration of elements or techniques that enable spatial and temporal control over hydrogels’ properties and functions. Here, we critically review the emerging progress of spatiotemporal control over biomaterial properties towards the development of functional engineered tissue constructs. Specifically, we will highlight the main advances in the spatial control of biomaterials, such as surface modification, microfabrication, photo-patterning, and three-dimensional (3D) bioprinting, as well as advances in the temporal control of biomaterials, such as controlled release of molecules, photocleaving of proteins, and controlled hydrogel degradation. We believe that the development and integration of these techniques will drive the engineering of next-generation engineered tissues.

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“…To date such systems have been utilized for numerous biomedical applications, including: peptide and protein delivery vehicles [56], cell delivery platforms with the capacity to modulate angiogenic paracrine responses [57], three-dimensional platforms (e.g., microfluidic or array) to study cell responses in vitro [58][59][60].…”

mentioning

confidence: 99%

Structured Substrates and Delivery Vehicles: Trending Now In Biomedicine

Pandit

Zeugolis

2016

Nanomedicine (Lond.)

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Biomedicine has entered the era of biofunctional biomaterials [1][2][3][4][5][6]. These are materials that utilize biophysical (e.g., topography [7][8][9][10][11][12]), biochemical (e.g., drug delivery [13-18]) and biological (e.g., gene delivery [19-24]) signals to control cellular functions in both in vitro and in vivo setting. In vitro, biofunctional materials aspire to either maintain the phenotype fidelity of permanently differentiated cells or direct stem cells toward specific lineage. In vivo, biofunctional materials desire to positively interact with host cells and surrounding tissues and promote functional repair and regeneration. The former frequently utilizes a structured substrate (e.g., electro-spun scaffolds, imprinted substrates) that would imitate the topographical/ architectural features of the tissue from which the cells were derived from (in the case of permanently differentiated cells) or the tissue that the cells are due to be implanted in (in the case of stem cells). The latter often uses a delivery vehicle (e.g., hydrogels, particles, dendrimers, micelles) that would support sustained and localized delivery of its cargo (e.g., cells, drugs, genes, growth factors).Electro-spun scaffolds and imprinted substrates have become an inherent element of modern biomedicine [25][26][27][28][29][30]. Indicative recent highlights of the electro-spinning technology include: formation of three-dimensional structures of controlled porosity [31], production of substrates for the differentiation of stem cells toward specific lineage [32], fabrication of delivery vehicles of biofunctional molecules for hypertrophic scars [33], development of high sensitivity acoustic sensors [34], assembly of high-performance lithium ion batteries [35]. Although nanoto microscale imprinted substrates did not bring about the anticipated effect in in vivo setting [36,37], numerous advances have been achieved recently, including: development of substrates with optimal dimensionality for osteogenic differentiation of human bone marrow stem cells [38], fabrication of electrode arrays to monitor the dopaminergic differentiation of human neural stem cells [39], production of electro-conductive nano-patterned substrates with enhanced myogenic differentiation and maturation capacity [40], construction of microfluidic devices [41], even manufacturing of high-performance lithium-ion micro-batteries [42]. The multimodal capacity/potential of structured technologies is undeniable. Refinement of engineering and manufacturing technologies in the years to come would enable more accurate and reproducible production of structured substrates at high volume and low cost, revolutionizing that way multiple disciplines, including biomedicine, filtration, imaging, energy and computing.Modern biomedicine requires localized and sustained delivery of bioactive molecules, therapeutic agents, trophic factors and viable cell populations to activate/enhance the innate reparative host capacity. The rationale of using delivery vehicles is based on the fa...

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Rapid Assembly of Heterogeneous 3D Cell Microenvironments in a Microgel Array

Cited by 97 publications

References 44 publications

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Logarithmic bacterial gradient chip for analyzing the effects of dietary restriction on C. elegans growth

Spatially and temporally controlled hydrogels for tissue engineering

Structured Substrates and Delivery Vehicles: Trending Now In Biomedicine

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