Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system

Tsou, Pei Hsiang; Sreenivasappa, Harini; Hong, Sungmin; Yasuike, Masayuki; Miyamoto, Hiroshi; Nakano, Keiyo; Misawa, T; Kameoka, Jun

doi:10.1016/j.bios.2010.06.034

Cited by 17 publications

(6 citation statements)

References 36 publications

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“…10 10 to 10 11 cm –2 ) and narrow pore size distribution that offers great opportunities for the creation of various nanostructured functional materials. In particular, large-scale photonic and plasmonic crystals for manipulation of light, highly permeable membranes for gas and liquid separation, − and templates for synthesis of ordered arrays of high-aspect-ratio nanostructures − are commonly prepared using anodic alumina films.…”

Section: Introductionmentioning

confidence: 99%

Crystallography-Induced Correlations in Pore Ordering of Anodic Alumina Films

Roslyakov¹,

Koshkodaev

Eliseev³

et al. 2016

J. Phys. Chem. C

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A crystallographic approach to tailoring the morphology and ordering degree of the porous structure of alumina films obtained by anodization of single-crystalline aluminum is discussed. The examination of porous structure of anodic alumina films formed on low-index and vicinal planes of Al single crystals under self-ordering conditions by high-resolution small-angle Xray scattering revealed the existence of two pore growth directions on vicinal facets. The inclination of channels from the normal to the metal surface is explained by the competitive impact of electromigration driving force and the crystallographic anisotropy of the substrate. It was also shown that pores growing in different directions during anodization retain hexagonal domains with various in-plane orientations. These results for the first time demonstrate the strong correlations between the longitudinal alignment and in-plane packing options of pores in anodic alumina films.

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Section: Introductionmentioning

confidence: 99%

Crystallography-Induced Correlations in Pore Ordering of Anodic Alumina Films

Roslyakov¹,

Koshkodaev

Eliseev³

et al. 2016

J. Phys. Chem. C

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“…9,13 PAO has also been applied for antimicrobial resistance or susceptibility testing of bacteria and fungi, giving more rapid results when compared to conventional tests such as disc diffusion and Etest. 15,16 The potential applications for PAO in microbiology and especially in culturing will expand if the PAO surface can be chemically modified to enable the attachment of biomolecules that can specifically interact with and bind to target bacteria. We have recently reported a modification approach that uses 1,ω-dialkynes to generate an alkyne-terminated monolayer on the PAO surface, which could be lactosylated via copper(I)catalyzed alkyne−azide cycloaddition (CuAAC), the "click" reaction.…”

Section: Introductionmentioning

confidence: 99%

“…The use of PAO in this application presents many advantages, such as the possibility to cultivate certain bacteria that do not grow on traditional agar plates (e.g., soil and river bacteria), , moving microcolonies from one culture medium to another, and the low autofluorescence of PAO that allows easy visualization of the cell growth by fluorescence microscopy. A commercially available microbial growth chip based on PAO, presenting up to one million compartments, even makes high-throughput screening of microorganisms possible. , PAO has also been applied for antimicrobial resistance or susceptibility testing of bacteria and fungi, giving more rapid results when compared to conventional tests such as disc diffusion and Etest. , …”

Section: Introductionmentioning

confidence: 99%

Stability of (Bio)Functionalized Porous Aluminum Oxide

et al. 2014

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Porous aluminum oxide (PAO), a nanostructured support for, among others, culturing microorganisms, was chemically modified in order to attach biomolecules that can selectively interact with target bacteria. We present the first comprehensive study of monolayer-modified PAO using conditions that are relevant to microbial growth with a range of functional groups (carboxylic acid, α-hydroxycarboxylic acid, alkyne, alkene, phosphonic acid, and silane). Their stability was initially assessed in phosphate-buffered saline (pH 7.0) at room temperature. The most stable combination (PAO with phosphonic acids) was further studied over a range of physiological pHs (4-8) and temperatures (up to 80 °C). Varying the pH had no significant effect on the stability, but it gradually decreased with increasing temperature. The stability of phosphonic acid-modified PAO surfaces was shown to depend strongly on the other terminal group of the monolayer structure: in general, hydrophilic monolayers were less stable than hydrophobic monolayers. Finally, an alkyne-terminated PAO surface was reacted with an azide-linked mannose derivative. The resulting mannose-presenting PAO surface showed the clearly increased adherence of a mannose-binding bacterium, Lactobacillus plantarum, and also allowed for bacterial outgrowth.

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“…The advantage of this method is the possibility of measuring bacteria in the range of 1 to 6 ϫ 10 4 cell counts; however, compared to the oCelloScope system, an AST can be performed for only one antibiotic at a time, and no image material that can reveal any erroneous results is available. The principles of several other methods of performing rapid ASTs have been presented (17)(18)(19)(20)(21)(22)31); a common limitation of these techniques is their inability to perform multiple-sample analysis.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Optical Antimicrobial Susceptibility Testing

et al. 2013

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Rapid antibiotic susceptibility testing is in high demand in health care fields as antimicrobial-resistant bacterial strains emerge and spread. Here, we describe an optical screening system (oCelloScope) which, based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effects within 6 min and within 30 min in complex samples from pigs suffering from catheter-associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method for detecting bacterial susceptibility that might entail an earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and might present new vistas as a point-of-care instrument in clinical and veterinary settings.

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Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system

Cited by 17 publications

References 36 publications

Crystallography-Induced Correlations in Pore Ordering of Anodic Alumina Films

Crystallography-Induced Correlations in Pore Ordering of Anodic Alumina Films

Stability of (Bio)Functionalized Porous Aluminum Oxide

Real-Time Optical Antimicrobial Susceptibility Testing

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