Rapid and Specific Detection of Hydroxyl Radical Using an Ultraweak Chemiluminescence Analyzer and a Low-Level Chemiluminescence Emitter:  Application to Hydroxyl Radical-Scavenging Ability of Aqueous Extracts of Food Constituents

Abstract

With the availability of an ultraweak chemiluminescence analyzer, it is possible to monitor the production of a specific oxygen-derived reactive species, such as hydroxyl radical ((*)OH), whenever a suitable chemiluminescent probe is obtainable. Reported herein is the development of a rapid and specific method for detecting (*)OH production using a specific probe, indoxyl-beta-glucuronide (IBG), a low-level chemiluminescence emitter. Using the Fenton reagent as a source of (*)OH, it was shown that IBG could elicit a very strong intensity of chemiluminescence (CL) (16200 +/- 200 photon counts/s). Conversely, IBG was shown to be insensitive to either superoxide radical or hydrogen peroxide with their CL intensities nearly close to the background values (25 +/- 5 and 180 +/- 20 photon counts/s, respectively). Furthermore, it was also shown that this IBG-based CL production could be effectively quenched by the addition of (*)OH scavengers such as sodium salicylate, dimethyl sulfoxide, and penicillamine to the assay system. Taken together, these data indicate that IBG is a specific CL probe suitable for monitoring the production of (*)OH. This system demonstrated inhibitory activities of various aqueous extracts of food constituents on the CL of hydroxyl radicals generated by Fenton's reagents with the order of scavenging efficiencies being Prunus mume > Cordyceps sinensin > Lilium lancifolium > Astragalus membranceus.

Experimental Studies on the Chemiluminescence Reaction Mechanism of Carbonate/Bicarbonate and Hydrogen Peroxide in the Presence of Cobalt(II)

“…26 Thiourea is an effective radical scavenger for • OH. 27 The inhibited CL signals were measured, respectively, after the addition of 20 µL of thiourea at different concentrations.…”

Study on the generation mechanism of reactive oxygen species on calcium peroxide by chemiluminescence and UV‐visible spectra

“…Furthermore, CL is an effective method in research on ROS, because of the associated instantaneous nature of CL and the short lifetime of ROS radicals (11)(12)(13)(14)(15)(16). Goto et al (17) successfully investigated the CL mechanism of the alkalineearth metal peroxides (MgO 2 , BaO 2 ) with CL reagents (luminol and lucigenin), and demonstrated that the hydroxyl ( • OH) and • O 2 − were released chiefly on the MgO 2 and BaO 2 , respectively, with CL.…”

“…Among the oxygen-centred free radicals, •OH is considered to be one of the most potent oxidizers (13). Thiourea is an effective radical scavenger for •OH (25).…”

Immobilized Cytochrome c Sensor in Organic/Aqueous Media for the Characterization of Hydrophilic and Hydrophobic Antioxidants

“…Some tests rely on photometric measurements of the concentration of artificial radicals, which absorb light at a suitable wavelength or react with additional chromophores [15]. Other methods use the ability of antioxidants to reduce luminol chemiluminescence [16,17]. The antioxidantmodulated damage inflicted on biomolecules by radicals can also serve as a test of the antioxidative properties [20,21].…”