Rapid and specific detection of intact viral particles using functionalized microslit silicon membranes as a fouling-based sensor

Abstract

The COVID-19 pandemic demonstrated the public health benefits of reliable and accessible point-of-care (POC) diagnostic tests for viral infections. Despite the rapid development of gold-standard reverse transcription polymerase chain reaction...

Nanomaterials for virus sensing and tracking

“…Silicon nanomembranes have an advantage over PCR for SARS-CoV-2 detection since they can selectively recognize intact virions thus differentiating between diseased and recently recovered individuals. 372 3.1.6 Other inorganic nanoparticles. Most metallic nanoparticles offer simplicity in surface modification in addition to a high surface area to volume ratio and the presence of localized surface plasmons.…”

“…368 The SiO 2 NPs–MIP nanocomposites show more than 95% cell viability after 24 hours and are therefore ideal for in vitro virus detection. 369,370 Silicon nanorods 371 and fouling-based silicon nanomembranes 372 have also been recently explored for virus detection although the use of these materials is not yet common due to scarce literature in the field. Silicon nanomembranes have an advantage over PCR for SARS-CoV-2 detection since they can selectively recognize intact virions thus differentiating between diseased and recently recovered individuals.…”

“…Silicon nanomembranes have an advantage over PCR for SARS-CoV-2 detection since they can selectively recognize intact virions thus differentiating between diseased and recently recovered individuals. 372…”

Rational design of multivalent biosensor surfaces to enhance viral particle capture

“…Pathogenic virus particle testing holds promise as a more rapid and accurate alternative to RNA polymerase chain reaction (PCR) from infected body parts. [1][2][3] Besides the transducer, the sensitivity of viral particle testing is largely dependent on the effective capture of the elastic bio-nanoparticles. [4][5][6] Therefore, a rationally designed biosensor surface is crucial for highly sensitive virus detection.…”

Rapid Assessment of Biomarkers on Single Extracellular Vesicles Using ‘Catch and Display’ on Ultrathin Nanoporous Silicon Nitride Membranes

“…38 The µSiM platform has also been used as a filter-based capture tool for diagnostic applications in an adaptation we term the ‘µSiM-DX’. 39 Here the µSiM- DX strategy is reconfigured to generate a pipette-powered microfluidic scheme for single EV analysis we call ‘ ca tch and d isplay for l iquid b iopsy (CAD-LB)’.…”