Rapid and Simultaneous Detection of Vitamin D Receptor Gene Polymorphisms by a Single ARMS-PCR Assay

Abstract

As ARMS-PCR is more rapid, economic, and user friendly than PCR-RFLP, its substitution would be welcomed in disease association and pharmacogenetic studies of VDR variants.

Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits

“…These results reflect higher accuracy of PCR-RFLP compared to T-ARMS-PCR, which results were consistent with the study by Li et al (2014). Based on sequencing experiments, the calculated accuracy of T-ARMS-PCR and PCR-RFLP reached 99.07% and 99.69%, respectively, which was very similar to the previous results Berenstein et al, 2014;Jafari et al, 2014). Therefore, T-ARMS-PCR is a reliable method for genotyping of SNP g.2885CNA within bovine ACADVL gene.…”

“…Moreover, 40% of the discrepancies between the two methods resulted from the PCR-RFLP incomplete digestion and the other 60% were the inaccuracies in sequencing of T-ARMS-PCR products . In the identification of mutations in IDH2 and DNMT3A, PCR-RFLP and ARMS methods showed a 100% concordance (Berenstein et al, 2014), and they also showed good uniformity in VDR genotypes in the sample of an Iranian population (Jafari et al, 2014). In the present study, the concordance between the two different methods was 98.76% (636/644).…”

Genotype Distribution of Vitamin D Receptor Polymorphisms among Indonesian Patients with Chronic Hepatitis B

“…Genomic DNA was extracted using Wizard Genomic DNA Purification kit (Promega Inc., Madison, WI, USA) according manufacturer's protocol. The VDR FokI T>C (rs 10735810), BsmI C>T (rs 1544410), ApaI G>T (rs7975232), and TaqI C>T (rs 731236) single nucleotide polymorphisms (SNPs) were determined by the Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) method using primers designed by Jafari et al (18). as presented in Table 1.…”

Oxidative stress and vitamin D receptor BsmI gene polymorphism in Egyptian children with systemic lupus erythematosus: a single center study

“…Genomic DNA extraction was done by Generation DNA Purification capture column kits (Fermentas, USA) from 200 µL of whole blood. The base sequences of the primers used for amplification-refractory mutation system polymerase chain reaction assay of VDR BsmI polymorphism were: 29 B′primer 5-′AGCCTGAGTACTGGGAATGT-3′;b′primer 5′-AGCCTGAGTACTGGGAATGC-3′;Consensus primer 5′-GGGAGGGAGTTAGGCACC-3′…”