Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging

Lee, Sangyeop; Chon, Hyangah; Lee, Ji-Young; Ko, Juhui; Chung, Bong Hyun; Lim, Dong Woo; Choo, Jaebum

doi:10.1016/j.bios.2013.07.063

Cited by 188 publications

(109 citation statements)

References 42 publications

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“…SERS dimer NPs with di-thiolated reporters functionalized with peptides were used to control binding and endocytosis, and were used in human glioblastoma cells for cancer targeted Raman imaging [309]. Multiplex SERS particles with several reporters [310,311] or additional fluorescence probes [312] have also been used to identify different cancer cells.…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

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Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

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Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

255

189

View full text Add to dashboard Cite

show abstract

“…Besides EGFR, Lee et al [52] used silica-encapsulated hollow gold nanospheres (SEHGNs) as a Raman nanotag that is conjugated with specific antibodies. This technique enables the assessment of the expression of epidermal growth factor (EGF), HER2, and insulin-like growth factor-1 receptors in the MDA-MB-468, KPL4, and SK-BR-3 human breast cancer cell lines.…”

Section: Composition Analysis Of Samples With Ramanmentioning

confidence: 99%

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

Gao

Han

et al. 2017

Journal of Spectroscopy

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Raman spectroscopy has been widely used as an important clinical tool for real-time in vivo cancer diagnosis. Raman information can be obtained from whole organisms and tissues, at the cellular level and at the biomolecular level. The aim of this paper is to review the newest developments of Raman spectroscopy in the field of breast cancer diagnosis and treatment. Raman spectroscopy can distinguish malignant tissues from noncancerous/normal tissues and can assess tumor margins or sentinel lymph nodes during an operation. At the cellular level, Raman spectra can be used to monitor the intracellular processes occurring in blood circulation. At the biomolecular level, surface-enhanced Raman spectroscopy techniques may help detect the biomarker on the tumor surface as well as evaluate the efficacy of anticancer drugs. Furthermore, Raman images reveal an inhomogeneous distribution of different compounds, especially proteins, lipids, microcalcifications, and their metabolic products, in cancerous breast tissues. Information about these compounds may further our understanding of the mechanisms of breast cancer.

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“…This method showed high sensitivity and specificity. [132] and [134] Lee and co-workers reported a SERS-based cellular imaging method for detection of breast cancer phenotypic markers expressed on cell surfaces ex vivo [146]. This method involved the synthesis of SERS nanotags including silicaencapsulated hollow Au nanospheres (SEHGNs) linked with specific antibodies.…”

Section: Ex Vivo Imaging Applicationsmentioning

confidence: 99%

SERS Nanotags and Their Applications in Biosensing and Bioimaging

et al. 2018

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Owing to the unique advantages of surface enhanced Raman scattering (SERS) in high sensitivity, specificity, multiplexing capability and photostability, it has been widely used in many applications, among which SERS biosensing and bioimaging are the focus in recent years. The successful applications of SERS for non-invasive biomarker detection and bioimaging under in vitro, in vivo and ex vivo conditions, offer significant clinical information to improve diagnostic and prognostic outcomes. This review provides recent developments and applications of SERS, in particular SERS nanotags in biosensing and bioimaging, describing case studies in which different types of biomarkers have been investigated, as well as outlining future challenges that need to be addressed before SERS sees both pathological and clinical use.

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Rapid and sensitive phenotypic marker detection on breast cancer cells using surface-enhanced Raman scattering (SERS) imaging

Cited by 188 publications

References 42 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

The Clinical Application of Raman Spectroscopy for Breast Cancer Detection

SERS Nanotags and Their Applications in Biosensing and Bioimaging

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