1976
DOI: 10.1016/s0021-9673(00)82252-9
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Rapid and sensitive high-resolution procedure for digitalis glycoside analysis by derivatization liquid chromatography

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1976
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Cited by 66 publications
(6 citation statements)
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“…In the HPLC analysis of digitalis glycosides, 4-nitrobenzoyl chloride has been used as a derivatizing agent in order to increase extinction values to a more favourable wavelength (Nachtmann et al, 1976). However, this principle has never before been used for the determination of solasodine.…”
Section: Discussionmentioning
confidence: 99%
“…In the HPLC analysis of digitalis glycosides, 4-nitrobenzoyl chloride has been used as a derivatizing agent in order to increase extinction values to a more favourable wavelength (Nachtmann et al, 1976). However, this principle has never before been used for the determination of solasodine.…”
Section: Discussionmentioning
confidence: 99%
“…The reproducibility of this technique is limited by the derivatization step and varies between 2.5 and 3.5% relative standard deviation for concentrations >100 ng/ml depending on the glycoside. The detection limits (3:1, signal-to-noise ratio) vary between 5 and 20 ng/ml at an injection volume of 100 µ (19).…”
Section: Methodsmentioning
confidence: 98%
“…The isocratic separation of this mixture of digitalis glycosides can be achieved in 12 min. The separation is sufficiently good for quantitative work (19). Impurities originating from the chloroform used in the extraction and traces of reagent are eluted before the derivatives.…”
Section: Methodsmentioning
confidence: 99%
“…The latter methods have included paper (14,15), thin-layer (16)(17)(18), column (19, 20), gas-liquid (21)(22)(23)(24)(25), and highperformance liquid chromatographic (HPLC) methods (26)(27)(28)(29)(30)(31)(32). Colorimetric methods are largely nonselective and lack high sensitivity.…”
mentioning
confidence: 99%