High-Performance Liquid Chromatographic Analysis of Digoxin Formulations

Desta, Belachew; McErlane, Keith M.

doi:10.1002/jps.2600710714

Journal of Pharmaceutical Sciences

1982

DOI: 10.1002/jps.2600710714

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High-Performance Liquid Chromatographic Analysis of Digoxin Formulations

Belachew Desta

Keith M. McErlane

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1988

2009

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Cited by 6 publications

References 22 publications

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Effects of Acanthopanax senticosus HARMS extract on drug transport in human intestinal cell line Caco-2

Takahashi¹,

Kaku

Satō³

et al. 2009

J Nat Med

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Acanthopanax senticosus HARMS (AS) is used as a Chinese herbal medicine and as a health supplement in Japan. However, little is known about the interaction between AS and other drugs. In this study, we investigated the effect of AS extract on intestinal drug transporter (P-glycoprotein, or P-gp) and peptide transporter activities in Caco-2 cells. Caco-2 cells were cultured on a culture dish and a permeable membrane for 1-3 weeks. The apical-to-basolateral (A-to-B) transport of digoxin, a P-gp substrate, was significantly increased by the addition of AS extract in a concentration-dependent manner. In contrast, the A-to-B transport of cephalexin, a peptide transporter substrate, was significantly decreased by the addition of AS extract in the same manner. The effects of AS extract addition on the kinetics of the uptake of rhodamine 123, a P-gp substrate, and Gly-Sar, a peptide transporter substrate, were investigated. V (max) for rhodamine 123 uptake was significantly increased by AS extract addition compared with the control, whereas that for Gly-Sar uptake was significantly decreased. On the other hand, K (m) and K (d) for rhodamine 123 and Gly-Sar uptake were not affected. We conducted further investigations to clarify the effect of AS extract addition on P-gp activity. When AS extract was added to the apical side, B-to-A transport of rhodamine 123 was significantly decreased compared with the control. Furthermore, the amount of intracellular rhodamine 123 was increased by AS extract addition compared with the control. These results suggest that P-gp and peptide transporter activities are suppressed by AS extract addition in a non-competitive manner.

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Effects of Acanthopanax senticosus HARMS extract on drug transport in human intestinal cell line Caco-2

Takahashi¹,

Kaku

Satō³

et al. 2009

J Nat Med

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Micro high-performance liquid chromatographic determination of cardiac glycosides in β-methyldigoxin and digoxin tablets

Fujii¹,

Ikeda²,

Yamazaki³

1988

Journal of Chromatography A

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Separation of cardiac glycosides by micellar electrokinetic chromatography and microemulsion electrokinetic chromatography

Debusschere

Demesmay

Rocca

et al. 1997

Journal of Chromatography A

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High-Performance Liquid Chromatographic Analysis of Digoxin Formulations

Cited by 6 publications

References 22 publications

Effects of Acanthopanax senticosus HARMS extract on drug transport in human intestinal cell line Caco-2

Effects of Acanthopanax senticosus HARMS extract on drug transport in human intestinal cell line Caco-2

Micro high-performance liquid chromatographic determination of cardiac glycosides in β-methyldigoxin and digoxin tablets

Separation of cardiac glycosides by micellar electrokinetic chromatography and microemulsion electrokinetic chromatography

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