Rapid and sensitive determination of Pseudomonas aeruginosa by using a glassy carbon electrode modified with gold nanoparticles and aptamer-imprinted polydopamine

Sarabaegi, Masoumeh; Roushani, Mahmoud

doi:10.1016/j.microc.2021.106388

Cited by 32 publications

(16 citation statements)

References 28 publications

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“…In P. aeruginosa cultures, the AgNCs constituted by F23 caused a decrease in the degree of structural alterations of the bacterium, resulting in a lower establishment of biofilms. [110] In 2021, Sarabaegi M. and Roushani M. [108] electrodeposited AuNPs on the surface of GCE, followed by the immobilization of F23 and previous electropolymerization of dopamine on the electrode; this modification had been shown to improve the affinity and selectivity of aptamers for their target. Thus, in the presence of bacteria, the modified electrode captured the pathogen, reducing the transfer of electrons captured by the electrode and achieving an LOD of 1 CFU • mL À 1 .…”

Section: Aptamer F23mentioning

confidence: 99%

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Gutiérrez‐Santana,

Coria‐Jiménez

2023

ChemMedChem

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Antimicrobial resistance (AMR) is a rapidly spreading global health problem, and approximately five million deaths associated with AMR pathogens were identified prior to the COVID‐19 pandemic. Pseudomonas aeruginosa has developed increasing AMR, and in patients with cystic fibrosis (CF) colonized by this bacterium, rare phenotypes have emerged that complicate the diagnosis and treatment of the hosts, in addition to multiple associated “epidemic strains” with high morbidities and mortalities. The conjugation of aptamers with fluorochromes or nanostructures has allowed the design of new identification strategies for Pseudomonas aeruginosa with detection limits of up to 1 cell ⋅ mL−1, and the synergy of aptamers with antibiotics, antimicrobial peptides and nanostructures has exhibited promising therapeutic qualities. Some selected aptamers against this bacterium have shown intrinsic antimicrobial activity. However, these aptamers have been poorly evaluated in clinical isolates and have shown decreased interactions for CF isolates, demonstrating, in these cases, uncommon phenotypes resulting from the selective qualities of this disease as well as the great adaptive capacity of the pathogen. Therefore, finding an aptamer or set of aptamers that have the ability to recognize strange phenotypes of this bacillus is crucial in the battle against AMR.

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Section: Aptamer F23mentioning

confidence: 99%

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Gutiérrez‐Santana,

Coria‐Jiménez

2023

ChemMedChem

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“…Pseudomonas aeruginosa (P. aeruginosa) can cause a long-term chronic disease, and low concentrations of P. aeruginosa can cause a serious infectious bacterium. Sarabaegi et al [ 47 ] designed an electrochemical sensor for the quantitative analysis of P. aeruginosa using MIP–aptamer. The polymeric substrates maintained the aptamer stability and selectivity.…”

Section: Application Of Mip–aptamer In the Molecular Recognition Of C...mentioning

confidence: 99%

Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Zhou

Liu

2022

Biosensors

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Molecular imprinting–aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer–aptamer (MIP–aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting–aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting–aptamer-combining technologies and summarized the mechanism of MIP–aptamer formation. Meanwhile, we discussed the challenges in preparing MIP–aptamer. Finally, we summarized the application of MIP–aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.

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“…A simple and effective strategy to potentially solve this problem is a double recognition strategy involving MIP and aptamer. Roushani et al, in particular, have advanced this field and used such a strategy to detect small molecules, proteins, and viruses. − For example, a nanohybrid probe was constructed based on double recognition by an aptamer-based MIP grafted onto a multiwalled carbon nanotubes-chitosan nanocomposites; when this probe was used to detect the core antigen of hepatitis C virus, it reached an impressive detection limit of 1.67 fg mL –1 . Similarly, an artificial receptor biosensor for the recognition of ultratrace trypsin was designed using a hybrid method including both MIP and aptamer technologies.…”

Section: Introductionmentioning

confidence: 99%

Non-autofluorescence Detection of H5N1 Virus Using Photochemical Aptamer Sensors Based on Persistent Luminescent Nanoparticles

Chen

Cai

Gong³

et al. 2022

ACS Appl. Mater. Interfaces

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Fluorescence sensing is limited in practical applications owing to multiple autofluorescent substances in complex biological samples such as serum. In this paper, the luminescence decay effect of persistent luminescent nanoparticles (PLNPs) was used to avoid the interference of autofluorescence in complex biological samples, and a non-autofluorescence molecularly imprinted polymer aptamer sensor (MIP-aptasensor) was designed to detect H5N1 virus. The proposed MIP-aptasensor consists of a magnetic MIP and aptamer-functionalized persistent luminescent nanoparticle Zn2GeO4:Mn2+-H5N1 aptamer (ZGO-H5N1 Apt). Upon simultaneous recognition of H5N1 virus, strong persistent luminescent signal changes were produced. Using the unique luminescent characteristics of PLNPs and the high selectivity of imprinted polymers and aptamers, the designed MIP-aptasensor effectively eliminates the autofluorescence background interference of serum samples and realizes the non-autofluorescence detection of H5N1 virus with high sensitivity (a limit of detection of 0.0128 HAU mL–1, 1.16 fM) and selectivity (the imprinting factor for the target H5N1 virus was 6.72). This tool provides a strategy for the design of sensors and their application in complex biological samples.

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Rapid and sensitive determination of Pseudomonas aeruginosa by using a glassy carbon electrode modified with gold nanoparticles and aptamer-imprinted polydopamine

Cited by 32 publications

References 28 publications

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Diagnosis and Therapeutic Strategies Based on Nucleic Acid Aptamers Selected against Pseudomonas aeruginosa: The Challenge of Cystic Fibrosis

Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Non-autofluorescence Detection of H5N1 Virus Using Photochemical Aptamer Sensors Based on Persistent Luminescent Nanoparticles

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