Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Abstract

Molecular imprinting–aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer–aptamer (MIP–aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting–aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting–aptamer-combining technologies and summarized the mechanism of MIP–aptamer formation. Meanwhile, we discussed the challenges in preparing MIP–aptamer. Finally, we summarized the application of MIP–aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.

A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

“…Due to the weak signal of mono-Apt, the concentration was increased to 200-3200 nM to ensure sufficient signal strength during detection. The Kd value was subsequently determined using the formula Y = B max X/(Kd + X), where X denotes the aptamer concentration, Y represents the CL intensity (obtained by subtracting the CL value of the blank group from the ELISA detection), and B max corresponds to the maximum Y observed during detection [16,29].…”

“…Aptamer is short, single-stranded DNA or RNA screened by exponential enrichment systems that can bind to targets with high affinity and specificity [16]. In recent years, aptamer has become a powerful tool for bacterial detection due to its small size, easy synthesis and labeling, strong affinity and specificity, and low production cost [17].…”

Construction of Aptamer-Molecularly-Imprinted Fluorescent Covalent Organic Framework and Its Collaborative Recognition of Diethylstilbestrol

“…Aptamers are recognition elements with multiple advantages such as easy functional modification, biocompatibility, wide application range, high affinity, etc. , Due to the respective advantages of MIPs and aptamers, many researchers have explored integrating MIPs with aptamers to build collaborative recognition systems, aiming to reduce the nonspecific binding of MIPs and protect aptamers by MIPs layers. The selectivity and affinity can be improved for the composite materials to the detection of the target substance, and the interference from complex matrix environments can also be reduced . Rad et al integrated a tetracycline (TET) aptamer and MIPs on gold nanoparticle-modified glass carbon electrodes to build an efficient electrical signal amplification platform with a detection limit of 144 fM for TET …”

“…The selectivity and affinity can be improved for the composite materials to the detection of the target substance, and the interference from complex matrix environments can also be reduced. 24 Rad et al integrated a tetracycline (TET) aptamer and MIPs on gold nanoparticle-modified glass carbon electrodes to build an efficient electrical signal amplification platform with a detection limit of 144 fM for TET. 25 In this assay, COFs with stable fluorescence were synthesized as fluorescent signals and further modified to act as a carrier to bind with the DES aptamer through covalent bonds and further synthesized MIPs.…”

An Overview on Recent Advances in Biomimetic Sensors for the Detection of Perfluoroalkyl Substances

“…MOFs can furthermore be combined with MIPs to improve their sensing properties [ 66 , 67 , 68 ], while also the combination of MOFs with aptamers has been reported in the literature [ 69 ]. For strategies to combine molecular imprinting with aptamer technology, we refer to the recent review article by Zhou and coworkers [ 70 ].…”