A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

Zhao, Yifan; Luo, Sheng; Qiao, Zhaohui; Zhou, Qianjin; Fan, Jianzhong; Lu, Jianfei; Chen, Jiong

doi:10.3390/fishes8100477

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…In another study, Zhao et al [59] developed a novel method for the detection of V. alginolyticus utilizing a specific aptamer for recognition and signal amplification via a hybridization chain reaction (HCR) and horseradish-peroxidase-conjugated streptavidin. The biotin-aptamer conjugate binds to streptavidin on horseradish-peroxidase-conjugated streptavidin (SA-HRP), which allows for a highly sensitive and specific detection method.…”

Section: Detection Of Vibrio Alginolyticusmentioning

confidence: 99%

Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification

Córdova-Espinoza,

González-Vázquez,

Barron-Fattel

et al. 2024

IJMS

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Early and accurate diagnoses of pathogenic microorganisms is essential to correctly identify diseases, treating infections, and tracking disease outbreaks associated with microbial infections, to develop precautionary measures that allow a fast and effective response in epidemics and pandemics, thus improving public health. Aptamers are a class of synthetic nucleic acid molecules with the potential to be used for medical purposes, since they can be directed towards any target molecule. Currently, the use of aptamers has increased because they are a useful tool in the detection of specific targets. We present a brief review of the use of aptamers to detect and identify bacteria or even some toxins with clinical importance. This work describes the advances in the technology of aptamers, with the purpose of providing knowledge to develop new aptamers for diagnoses and treatment of different diseases caused by infectious microorganisms.

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Section: Detection Of Vibrio Alginolyticusmentioning

confidence: 99%

Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification

Córdova-Espinoza,

González-Vázquez,

Barron-Fattel

et al. 2024

IJMS

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Aptasensors and Advancement in Molecular Recognition Technology

Napit,

Jaysawal,

Chowdhury

et al. 2024

Adv Materials Technologies

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Synthetic oligonucleic acids known as aptamers exhibit remarkable selectivity and affinity for target recognition and binding. Selected via an iterative process known as “selective evolution of ligands by exponential enrichment” (SELEX), aptamers fold into defined 3D conformations to interact with their targets. The incorporation of aptamers as recognition elements has driven notable progress in biosensors, giving rise to the development of aptasensors. Here, the process of aptamer discovery and the development of various types of aptasensors are summarized. The fundamental design principles of aptasensors are elaborated along with the superiority of aptamers compared to antibodies. The various modes employed by aptasensors, such as structure‐switching design, hybridization chain reaction amplification, enzyme‐assisted recycling, and split aptamer design are examined. Further light is shed on the diverse landscape of aptasensors, their adaptability to different analytes aptasensors as well as their potential to propel advancements in modern biosensor technology. As a nucleic acids‐based biosensor platform, aptasensors poise to become a next generation of sensitive and cost‐effective technology to shape the future of molecular recognition in biosensing.

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A Novel Method for Sensitive Detection of Vibrio alginolyticus Based on Aptamer and Hybridization Chain Reaction in Aquaculture

Cited by 2 publications

References 30 publications

Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification

Aptamers: A Cutting-Edge Approach for Gram-Negative Bacterial Pathogen Identification

Aptasensors and Advancement in Molecular Recognition Technology

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