Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal amplification combined with a lateral-flow dipstick

Nimitphak, Tongchai; Meemetta, Watcharachai; Arunrut, Narong; Senapin, Saengchan; Kiatpathomchai, Wansika

doi:10.1016/j.mcp.2009.09.004

Cited by 46 publications

(30 citation statements)

References 19 publications

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“…Visual detection of test results by this method abrogates the need for post-amplification hybridization step for DNA products and probes required for LFD methods previously combined with LAMP for shrimp virus detection (Kiatpathomchai et al, 2008;Jaroenram et al, 2009;Puthawibool et al, 2009;Nimitphak et al, 2010;Arunrut et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome

Jaroenram

Owens

2014

Journal of Virological Methods

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Section: Discussionmentioning

confidence: 99%

Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome

Jaroenram

Owens

2014

Journal of Virological Methods

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“…Nimitphak et al ( 2010 ) developed a rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal amplifi cation combined with a lateralfl ow dipstick . With a DNA template extracted from PemoNPV-infected shrimp, the LAMP-LFD detection limit was 0.1 pg, whereas one-step PCR and nested PCR followed with gel electrophoresis was 1 pg.…”

Section: Other Animal Virusesmentioning

confidence: 99%

Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

Saharan

Khatri

Dingolia

et al. 2013

Biotechnology: Prospects and Applications

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Most of the diseases caused by viral infection are found to be fatal, and the diagnosis is diffi cult due to confusion with other causative agents. So, a highly effi cient molecular-based advance detection technique, i.e., loopmediated isothermal amplifi cation (LAMP) method, is developed for diagnosis of viral infections by various workers. It is based on amplifi cation of DNA at very low level under isothermal conditions, using a set of four specifi cally designed primers and a DNA polymerase with strand displacement activity. This technique is found to be superior than most of the molecular techniques like PCR, RT-PCR, and real-time PCR due to its high specifi city, sensitivity, and rapidity. Major advantage of LAMP method is its cost-effectiveness as it can be done simply by using water bath or dry bath. Here, in this review information regarding almost all the effective LAMP techniques which is developed so far for diagnosis of numerous viral pathogens is presented.

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“…Lateral flow tests, sometimes referred to as dipsticks, are used in many environmental and healthcare applications (e.g., colorimetric pregnancy tests) [13] . For example, lateral flow tests have been used for botulinum neurotoxin, aflatoxin B1, and virus detection [14][15][16][17] . One such lateral flow test is the Reveal ® for…”

Section: Introductionmentioning

confidence: 99%

Microstereolithography-fabricated microneedles for fluid sampling of histamine-contaminated tuna

Boehm¹,

Jaipan

Yang

et al. 2024

IJB

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Abstract:A custom-designed microneedle sampling system was prepared using dynamic mask microstereolithography; this sampling system was used for determination of histamine content in fresh, histamine-spiked, and spoiled tuna flesh. Lateral flow (test strip) assays were successfully utilized in the microneedle sampling system to assess histamine content. Good agreement was noted between data obtained from the microneedle sampling system and a commercially available histamine detection kit. A discrepancy was noted in the results from the microneedle sampling system and the commercially available histamine detection kit at low (negative) levels of histamine. There was an improvement in the agreement between the microneedle sampling system and the commercially available histamine detection kit at higher histamine levels. The results, which showed an improvement in the test duration and the amount of reagent needed for histamine detection, indicate the promise of printed microneedle sampling systems for histamine detection in seafood samples and other types of food testing.

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Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal amplification combined with a lateral-flow dipstick

Cited by 46 publications

References 19 publications

Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome

Recombinase polymerase amplification combined with a lateral flow dipstick for discriminating between infectious Penaeus stylirostris densovirus and virus-related sequences in shrimp genome

Rapid Detection of Viruses Using Loop-Mediated Isothermal Amplification (LAMP): A Review

Microstereolithography-fabricated microneedles for fluid sampling of histamine-contaminated tuna

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