Rapid and reversible control of human metabolism by individual sleep states

Nowak, Nora; Gaisl, Thomas; Miladinovic, Djordje; Marcinkevičs, Ričards; Osswald, Martin; Bauer, Stefan; Buhmann, Joachim M.; Zenobi, Renato; Sinués, Pablo Martínez-Lozano; Brown, Steven A.; Kohler, Malcolm

doi:10.1016/j.celrep.2021.109903

Cited by 34 publications

(33 citation statements)

References 71 publications

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“…SESI sources are commercially available and can be interfaced with commercial high-resolution mass spectrometers [27, 28, 56]. The data generated from SESI-MS is compatible with standard software for direct-injection MS-based metabolomics [57, 58], and online MS2 fragmentation for compound identification can be performed [59, 60]. Development is still needed for collecting and processing time-series data, since most current MS-based metabolomics datasets have low time-resolution or do not consider time-dependent changes at all.…”

Section: Discussionmentioning

confidence: 99%

“…Development is still needed for collecting and processing time-series data, since most current MS-based metabolomics datasets have low time-resolution or do not consider time-dependent changes at all. One example where time series of SESI-MS data at a minute-level resolution has been successfully used was for continuous monitoring of human metabolism during sleep [57].…”

Section: Discussionmentioning

confidence: 99%

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Non-invasive monitoring of microbiota and host metabolism using Secondary electrospray ionization-Mass spectrometry

Lan

Greter

Streckenbach

et al. 2022

Preprint

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The metabolic “handshake” between the microbiota and its mammalian host is a complex, dynamic process with potentially major influences on health. Dissecting the interaction between microbial species/strains and metabolites found in host tissues has been a challenge due to the high diversity of a complete micro-biota and the requirement for invasive sampling, which precludes high-resolution longitudinal analysis. Here we demonstrate that secondary electrospray ionization mass spectrometry can be used to non-invasively monitor metabolic activity of the intestinal microbiome of a live, awake mouse. This was achieved via analysis of the headspace volatile and semi-volatile metabolome of individual gut microbiota bacterial species growing in pure culture, as well as from live gnotobiotic mice specifically colonized with these microbes (i.e. metabolites released to the atmosphere via breath, the skin and from the gut). The microbial origin of these compounds was confirmed by feeding of heavy-isotope labeled microbiota-accessible sugars. This reveals that the microbiota is a major contributor to the released metabolites of a whole live mouse, and that it is possible to capture the catabolism of sugars and cross-feeding within the gut microbiota of a living animal using volatile/semi-volatile metabolite monitoring.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Non-invasive monitoring of microbiota and host metabolism using Secondary electrospray ionization-Mass spectrometry

Lan

Greter

Streckenbach

et al. 2022

Preprint

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“…We hypothesised that measured intensities correlate linearly with exhaled metabolite concentration. A linear correlation between slightly volatile metabolite concentrations in blood and exhaled breath samples has been reported [19,44]. The contrasting pre-topost-exercise patterns in metabolites measured in breath versus plasma samples cannot be easily explained, and will require additional research.…”

Section: Discussionmentioning

confidence: 99%

Real-Time Monitoring of Metabolism during Exercise by Exhaled Breath

et al. 2021

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Continuous monitoring of metabolites in exhaled breath has recently been introduced as an advanced method to allow non-invasive real-time monitoring of metabolite shifts during rest and acute exercise bouts. The purpose of this study was to continuously measure metabolites in exhaled breath samples during a graded cycle ergometry cardiopulmonary exercise test (CPET), using secondary electrospray high resolution mass spectrometry (SESI-HRMS). We also sought to advance the research area of exercise metabolomics by comparing metabolite shifts in exhaled breath samples with recently published data on plasma metabolite shifts during CPET. We measured exhaled metabolites using SESI-HRMS during spiroergometry (ramp protocol) on a bicycle ergometer. Real-time monitoring through gas analysis enabled us to collect high-resolution data on metabolite shifts from rest to voluntary exhaustion. Thirteen subjects participated in this study (7 female). Median age was 30 years and median peak oxygen uptake (VO2max) was 50 mL·/min/kg. Significant changes in metabolites (n = 33) from several metabolic pathways occurred during the incremental exercise bout. Decreases in exhaled breath metabolites were measured in glyoxylate and dicarboxylate, tricarboxylic acid cycle (TCA), and tryptophan metabolic pathways during graded exercise. This exploratory study showed that selected metabolite shifts could be monitored continuously and non-invasively through exhaled breath, using SESI-HRMS. Future studies should focus on the best types of metabolites to monitor from exhaled breath during exercise and related sources and underlying mechanisms.

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“…Both volatile compounds from the environment and exhaled breath were detected, in addition to non‐volatile analytes likely derived from aerosolized saliva. Finally, in a recent study by Nowak et al., SESI‐MS was employed to perform real‐time measurements of human breath throughout the sleep cycle 149 . Almost 2000 metabolites were studied, demonstrating a complex mediation of major metabolic pathways as numerous metabolites altered their concentration in sleep and wake states.…”

Section: Applications Of Ambient Ionization Mass Spectrometrymentioning

confidence: 99%

Applications of ambient ionization mass spectrometry in 2021: An annual review

Rankin‐Turner

Reynolds

Turner

et al. 2022

Analytical Science Advances

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Ambient ionization mass spectrometry (AIMS) has revolutionized the field of analytical chemistry, enabling the rapid, direct analysis of samples in their native state. Since the inception of AIMS almost 20 years ago, the analytical community has driven the further development of this suite of techniques, motivated by the plentiful advantages offered in addition to traditional mass spectrometry. Workflows can be simplified through the elimination of sample preparation, analysis times can be significantly reduced and analysis remote from the traditional laboratory space has become a real possibility. As such, the interest in AIMS has rapidly spread through analytical communities worldwide, and AIMS techniques are increasingly being integrated with standard laboratory operations. This annual review covers applications of AIMS techniques throughout 2021, with a specific focus on AIMS applications in a number of key fields of research including disease diagnostics, forensics and security, food safety testing and environmental sciences. While some new techniques are introduced, the focus in AIMS research is increasingly shifting from the development of novel techniques toward efforts to improve existing AIMS techniques, particularly in terms of reproducibility, quantification and ease-of-use.

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Rapid and reversible control of human metabolism by individual sleep states

Abstract: Highlights d Sleep states regulate more than 50% of all metabolite features detected in human breath d Major pathways are up-or downregulated during wake, SWS, and REM sleep d Sleep-regulated pathways show stepwise logic across different sleep stages

Cited by 34 publications

References 71 publications

Non-invasive monitoring of microbiota and host metabolism using Secondary electrospray ionization-Mass spectrometry

Non-invasive monitoring of microbiota and host metabolism using Secondary electrospray ionization-Mass spectrometry

Real-Time Monitoring of Metabolism during Exercise by Exhaled Breath

Applications of ambient ionization mass spectrometry in 2021: An annual review

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