Rapid and Reliable Detection and Identification of GM Events Using Multiplex PCR Coupled with Oligonucleotide Microarray

Xu, Xiaodan; Li, Yingcong; Zhao, Heng; Wen, Siyuan; Wang, Shengqi; Huang, Jiehong; KunLun, Huang; Luo, Yunbo

doi:10.1021/jf048368t

Cited by 36 publications

(19 citation statements)

References 14 publications

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“…Furthermore, the primer used to identify soybeans must be well-known. The primer used in this study has been used in various previous articles (Meyer et al 1996;Burns et al 2003;Greiner et al 2005;Xu et al 2005;Abd El-Nasser et al 2010). …”

Section: Test Specificity and Sensitivitymentioning

confidence: 99%

Specific identification of chicken and soybean fraud in premium burgers using multiplex-PCR method

Tafvizi

Hashemzadegan

2015

J Food Sci Technol

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The increased consumption of meat products, such as hamburger in large cities such as Tehran, has highlighted the importance of quality control for these products. Due to the escalating cost of red meat, and the difficulty of detecting adulteration in ground meat, the replacement of red meat with cheaper animal and plant proteins in these products is clearly possible. As a result, the aim of this study was to investigate the validity of labeling in premium hamburgers made of beef. In addition, the presence of soybean and chicken meat, which constitutes commercial fraud in premium hamburgers, was detected using a sensitive and quick multiplex-PCR method. In total, 10 specified brands of premium hamburgers purported to consist of beef were collected from markets in Tehran City, Iran. DNA was extracted from the premium hamburgers, then, simplex-PCR and multiplex-PCR fwere optimized using specific beef, chicken and soybean primers. The 118, 183, and 274 bp fragments, were amplified in all samples from soybean lectin, 12 s rRNA, and mitochondrial cytochrome b genes, respectively. The results indicated the addition of chicken meat and soybean in the premium hamburgers which were not indicated on their labels.

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Section: Test Specificity and Sensitivitymentioning

confidence: 99%

Specific identification of chicken and soybean fraud in premium burgers using multiplex-PCR method

Tafvizi

Hashemzadegan

2015

J Food Sci Technol

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“…This array employed a microporous, hydrophobic polyester cloth as a solid support. Similarly, an array containing features based on 8 structural genes has been demonstrated to be informative for the identification of RR soybean (Xu et al 2005), and has recently been extended to include 20 genetic elements (Xu et al 2006). This system not only tells whether the sample is made of GMOs, but it can also distinguish which kind of plant it belongs to and which characteristics it has, like insect and herbicide resistance.…”

Section: Genetically Modified Organism Detection In Foodmentioning

confidence: 99%

Array Platform for Food Safety and Quality

Consolandi¹,

Cremonesi²,

Bordoni³

et al. 2012

OMICs Technologies

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“…A DNA microarray is important as a high-throughput assay, and has already been used for GMO detection [25][26][27][28][29]. In Korea, a DNA microarray chip was developed for the detection of 24 GMOs, which include 2 GM soybeans (GTS-40-3-2 and A2704-12), 13 GM maizes (Bt176, Bt11, MON810, MON863, NK603, GA21, T25, TC1507, Bt10, DAS59122-7, TC6275, MIR604, and LY038), 3 GM canolas (GT73, MS8xRF3, and T45), 5 GM cottons (MON1445, MON 15985, MON 531, MON 88913, and LLcotton25), and 1 GM rice (Shanyou 63).…”

Section: Microarraymentioning

confidence: 99%

Regulation and detection methods for genetically modified foods in Korea

Kim

2010

Pure and Applied Chemistry

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Genetically modified organisms (GMOs) have been developed and commercialized in many countries for the past decade. The regulations on these GMOs in Korea have been established through the labeling and safety evaluation systems for management of genetically modified (GM) foods under the Food Sanitation Act enacted by the Korea Food and Drug Administration (KFDA). To manage these regulations effectively, several methods for GMO detection, including polymerase chain reaction (PCR) methods and immunoassay, are applied. For detection of GMOs at the level of DNA, primers from the promoter, terminator, other marker genes, and expressed proteins used in a wide range of GMOs were designed for single, multiplex, real-time PCR, and microarray applications. In Korea, multiplex PCR methods specific to GM soybean, maize, canola, and cotton were designed. The limit of detection (LOD) value was determined to be 1 % of GM mixtures, which is a significant value for the PCR method used for the labeling threshold in Korea. A DNA microarray chip was also developed for the detection of 24 GMOs, including GM soybeans, 13 GM maizes, 3 GM canolas, 5 GM cottons, and 1 GM rice. This DNA chip was proven to successfully detect GMOs from raw and processed foods. In the near future, more powerful screening and detection methods are needed for handling many kinds of GMOs and unauthorized GMOs.

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Rapid and Reliable Detection and Identification of GM Events Using Multiplex PCR Coupled with Oligonucleotide Microarray

Cited by 36 publications

References 14 publications

Specific identification of chicken and soybean fraud in premium burgers using multiplex-PCR method

Specific identification of chicken and soybean fraud in premium burgers using multiplex-PCR method

Array Platform for Food Safety and Quality

Regulation and detection methods for genetically modified foods in Korea

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