Rapid and Inexpensive Colorimetric Detection of Total Serum Protein Using Microzone Plates Wax-Printed on Polyester Films

Paper microzone assay embedded on a 3D printed support for colorimetric quantification of proteins in different biological and food samples

“…3,4 Considering their spread and usefulness and the growing demand for portable and low-cost procedures, an increasing number of attempts have been made to develop and optimize alternative colorimetric assays for protein detection, built on paper-based analytical devices (PADs). [5][6][7][8][9][10][11] PADs are a class of "lab-on-chip" sensors mainly made of cellulosic materials in which one or more hydrophilic regions (detection zone or microzone) are patterned by the creation of impermeable barriers, in order to delimitate an analytical zone in which a specic assay is to be performed. 12 The optimization of an analytical assay in a PAD has been proven to have several advantages when compared to conventional approaches.…”

Quick and Cheap Colorimetric Quantification of Proteins Using 96-Well-Plate Images

“…Nowadays, simple, and inexpensive electronic instruments, such as smartphones, digital cameras, and desktop scanners, have been used for colorimetric measurements. 6 Several laboratory practices describing quantitative colorimetry using electronic instruments were published in this Journal. These publications were reviewed by Kovarik et al, 7 and, since that, some laboratory experiments have been published such as starch quantification in ripe banana, 8 determination of biodiesel in diesel blends, 9 analysis of salivary amylase using starch from food as a substrate, 10 iron(III) determination in water, 11,12 quantification of colored household products, 13 determination of ciprofloxacin, 14 and acetylsalicylic acid in pharmaceuticals, 15 exploration of chemical Kinetic of the bleaching reaction of RD40, 16 building a Visible Spectrophotometer, 17 fluorescence using homemade equipment, 18 finding the pK a values of thymol blue, 19 quantification of Allura Red in maraschino cherry juice, 20 construction of adsorption isotherms, 21 determination of glucose, 22 and quantification of Cu 2+ in solution.…”

“…Normally, it is carried out using photometers, spectrophotometers, and 96-well-plate readers. Nowadays, simple, and inexpensive electronic instruments, such as smartphones, digital cameras, and desktop scanners, have been used for colorimetric measurements . Several laboratory practices describing quantitative colorimetry using electronic instruments were published in this Journal.…”