Rapid and highly specific detection of communicable pathogens using one-pot loop probe-mediated isothermal amplification (oLAMP)

Disposable and low-cost pen-like sensor incorporating nucleic-acid amplification based lateral-flow assay for at-home tests of communicable pathogens

“…To achieve the accurate and visible nucleic acid tests (NATs), we integrated our previously published oLAMP reaction ( Lu et al, 2022 ) with lateral-flow assays (LFA) as signal readout, allowing user-oriented and visible NAT for pathogens detection. The inner loop-probe primer in oLAMP was modified into a FAM-biotin-labeled loop-probe.…”

“…The primer and probe design, loop probe cleavage assay and oLAMP reaction were all conducted according to our previous work ( Lu et al, 2022 ). The primers and probes used in this study listed in Table S1 were purchased from Generay.…”

“…Previously, a one-pot loop probe-mediated isothermal amplification reaction (oLAMP) was developed by our group ( Lu et al, 2022 ). Unlike other multi-step NATs, we introduced a fluorophore-quencher-labeled loop probe which acts as a target-specific primer and as a reporter that is cleaved by a nicking endonuclease in the presence of the target.…”

Target-triggered cascade signal amplification for sensitive electrochemical detection of SARS-CoV-2 with clinical application

“…Song et al [ 21 ] have proposed an antifouling electrochemical biosensor for SARS-CoV-2 detection by introducing the polymerized polyaniline nanowires and elaborate designed inverted Y-peptides, which can successfully detect SARS-CoV-2 at a low concentration of 3.5 fM. Lu et al [ 22 ] have integrated the loop probe-mediated isothermal amplification strategy with electrochemical technology to analyze the SARS-CoV-2 in one-pot to avoid cross-contamination, which realizes fast readout within 30–40 min, with an ultra-low detection limit of 1 copy/μL. Nonetheless, among these methods, the cost and convenience may be sacrificed because of either the involvement of the design of probes modified with additional electroactive labels or the complex synthesis procedure of nanomaterials.…”

Immunoassay platform with surface-enhanced resonance Raman scattering for detecting trace levels of SARS-CoV-2 spike protein

“…For diagnosis of Covid-19 through detection of nucleocapsid protein, Chen et al [ 25 ] constructed an electrical double layer biosensor, while Qi et al [ 26 ] designed a fast-responding aptasensor based on a low-cost microelectrode array chip. RNA fragments have also been applied in detecting Covid-19 with a one-pot loop probe-mediated isothermal amplification method [ 27 ]. There are serological tests which can be used for early diagnosis, for they can detect viral proteins at the onset of symptoms.…”