Disposable and low-cost pen-like sensor incorporating nucleic-acid amplification based lateral-flow assay for at-home tests of communicable pathogens

Lu, Xiao; Lin, Haosi; Feng, Xianzhen; Lui, Grace CY.; Hsing, I‐Ming

doi:10.1016/j.biosx.2022.100248

Cited by 4 publications

(4 citation statements)

References 23 publications

(24 reference statements)

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“…Therefore, aerosol-induced cross-contamination is a potential issue in real-world settings during the manual transfer and mixing of the LAMP product with LFA buffer, a critical step in the diagnostic process. Recently, various devices have been developed to facilitate safer and more reliable handling of bio-samples in non-laboratory settings [41][42][43]. These devices significantly reduce the risk of contamination by preventing aerosolization of the sample, thus maintaining the integrity of the assay.…”

Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Jang,

Lee,

Lee

et al. 2024

Diagnostics

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Influenza viruses cause highly contagious respiratory diseases that cause millions of deaths worldwide. Rapid detection of influenza viruses is essential for accurate diagnosis and the initiation of appropriate treatment. We developed a loop-mediated isothermal amplification and lateral flow assay (LAMP-LFA) capable of simultaneously detecting influenza A and influenza B. Primer sets for influenza A and influenza B were designed to target conserved regions of segment 7 and the nucleoprotein gene, respectively. Optimized through various primer set ratios, the assay operated at 62 °C for 30 min. For a total of 243 (85 influenza A positive, 58 influenza B positive and 100 negative) nasopharyngeal swab samples, the performance of the influenza A/B multiplex LAMP-LFA was compared with that of the commercial AllplexTM Respiratory Panel 1 assay (Seegene, Seoul, Korea). The influenza A/B multiplex LAMP-LFA demonstrated a specificity of 98% for the non-infected clinical samples, along with sensitivities of 94.1% for the influenza A clinical samples and 96.6% for the influenza B clinical samples, respectively. The influenza A/B multiplex LAMP-LFA showed high sensitivity and specificity, indicating that it is reliable for use in a low-resource environment.

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Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Jang,

Lee,

Lee

et al. 2024

Diagnostics

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“… 47 , 48 In addition, point-of-care or field-deployable MPXV diagnostics will require rapid and easy sample preparation. A few methods of sample preparations have been reported for point-of care or field-deployable diagnostics, including one-step sample extraction with TNA-Cifer Reagent, 49 using dipstick extraction technology, 50 and disposable pen-like sensor systems, 51 which can be tested along with our mpox detection assay. Our RPA-Cas12a system can be optimized by incorporating the visual readout component and one-step sample preparations with the aim for field and home use.…”

Section: Discussionmentioning

confidence: 99%

Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

Zhao,

Hu,

Fan

et al. 2023

Cell Reports Methods

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“…The W1209 module used in the MobileLAMP is an affordable ($1.5) temperature controller that can be easily obtained even in single quantities. Note that the W1209 module was used in a LAMP incubation device recently (38), however does not include detailed working of the module which we have covered in this work. The heat block used in MobileLAMP is a widely used aluminium block in FDM 3D-printers, which can be purchased for as little as $1 even in low quantities.…”

Section: Affordabilitymentioning

confidence: 99%

MobileLAMP: A portable, low-cost, open-source device for isothermal nucleic acid amplification

Bhupathi,

Hegde,

Devarapu

et al. 2024

Preprint

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Isothermal amplification-based methods for pathogen DNA or RNA detection offer high sensitivity, rapid detection, and the potential for deployment in remote fields and home testing. Consequently, they are emerging as alternatives to PCR and saw a surge in research activity and deployment for the rapid detection of SARS-CoV-2 during the Covid-19 pandemic. The most common isothermal DNA detection methods rely on minimal reagents for DNA amplification and simple hardware that can maintain isothermal conditions and read-out a fluorescent or colorimetric signal. Many researchers globally are working on improving these components based on diverse end-user needs. In this work, we have recognized the need for an open-source hardware device for isothermal amplification, composed of off-the-shelf components that are easily accessible in any part of the world, is easily manufacturable in a distributed and scalable way using 3D printing, and that can be powered using a wide diversity of batteries and power sources. We demonstrate the easy assembly of our device design and demonstrate its efficacy using colorimetric LAMP for both RNA and DNA targets.

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Disposable and low-cost pen-like sensor incorporating nucleic-acid amplification based lateral-flow assay for at-home tests of communicable pathogens

Cited by 4 publications

References 23 publications

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Loop-Mediated Isothermal Amplification and Lateral Flow Immunochromatography Technology for Rapid Diagnosis of Influenza A/B

Rapid and sensitive one-tube detection of mpox virus using RPA-coupled CRISPR-Cas12 assay

MobileLAMP: A portable, low-cost, open-source device for isothermal nucleic acid amplification

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