Rapid and Convenient Detection of Urinary Equol by Thin-Layer Chromatography

Yokoyama, Shin-ichiro; Kuzuguchi, Tsuyoshi

doi:10.3177/jnsv.53.43

Cited by 6 publications

(3 citation statements)

References 20 publications

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“…TLC was used to screen isoflavones by following a previously published method [ 10 ] with a slight modification. Of the approximately 200 colonies from plates of various media, 3 had a TLC band indicating the presence of equol molecules in the culture.…”

Section: Resultsmentioning

confidence: 99%

“…TLC experiments to screen for isoflavones were performed with the following method from a previous publication [ 10 ] with slight modifications. In brief, bacterial culture supernatant was transferred to RP-18 silica gel TLC plates (20 × 20 cm, Merck, 60 F, 254 nm, Darmstadt, Germany) with a solvent system of acetonitrile: water: acetic acid (60:40:1).…”

Section: Methodsmentioning

confidence: 99%

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Isolation and identification of new bacterial stains producing equol from Pueraria lobata extract fermentation

et al. 2018

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Equol is a nonsteroidal estrogen that is produced by intestinal bacterial metabolism. Equol and equol-producing bacteria have been extensively investigated with soybean-based materials under anaerobic condition. In this study, an under-appreciated plant material, Pueraria lobata, was used to find new bacterial strains that produce equol under aerobic conditions. Three new intestinal bacteria, CS1, CS2, and CS3, were isolated, and internal transcribed spacer analysis revealed that belonging to genus Pediococcus and Lactobacillus. HPLC analysis showed that these strains produced equol or its related intermediates when fermenting P. lobata extract. In comparison to fermentation of P. lobata extract, soybean germ extract was also fermented. While the isolated strains did not produce equol in this extract, they produced other equol-related precursors. To test the modularity effect of these fermentation mixtures with the newly isolated bacteria, MCF-7 cell proliferation assay was performed, which showed that all extracts fermented with those strains has a modularity effect. Fermenting P. lobata extract with strain CS1 demonstrated the best modularity effect.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Isolation and identification of new bacterial stains producing equol from Pueraria lobata extract fermentation

et al. 2018

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“…We began our study of soy isoflavonoid metabolism by human intestinal bacteria because the bacterial product equol has activity different from that of the substrate daidzein [14]. We have also compared the activities of the soy isoflavonoid metabolites with those of daidzein and genistein [5,15].…”

Section: Resultsmentioning

confidence: 99%

Cleavage of Methyl Ethers by O-Desmethylangolensin-Producing Clostridium Strain SY8519

Niwa¹,

Yokoyama²,

Kawada³

et al. 2015

J Microb Biochem Technol

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We previously isolated an O-desmethylangolensin (O-DMA)-producing bacterium, Clostridium rRNA cluster XIVa strain SY8519. We studied the metabolism of soy isoflavonoids by strain SY8519 and found that the bacterium attenuated the activity of isoflavonoids by "decomposition". In this study, the O-methyl derivatives of isoflavones formononetin and biochanin A were fed to the bacterium. Formononetin and biochanin A were metabolized to O-DMA and 2-(4-hydroxyphenyl) propionic acid, respectively, which are the products of the original isoflavones. We could detect daidzein as an intermediate in the production of O-DMA from formononetin by time course analysis of the culture medium. Therefore, strain SY8519 can cleave the O-methyl ethers of isoflavones. We then examined the demethylation of O-methyl derivatives of gallic acid methyl ester by strain SY8519. The metabolite was almost 3-O-methylgallic acid methyl ester but not gallic acid ester. These results suggest that strain SY8519 also cleaves the O-methyl ethers of certain phenolic compounds by demethyl enzyme. We also compared the activities of the O-methyl derivatives of gallic acid methyl ester. As the number of the methyl ether decreased, the activities in tyrosinase inhibition and antioxidant assay increased. Therefore, compared with humans who have different microflora, humans who have strain SY8519 or who have similar microbial activity in their intestine would experience different effects after the intake of phytochemicals.

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Characterization of an O-desmethylangolensin-producing bacterium isolated from human feces

et al. 2009

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Rapid and Convenient Detection of Urinary Equol by Thin-Layer Chromatography

Cited by 6 publications

References 20 publications

Isolation and identification of new bacterial stains producing equol from Pueraria lobata extract fermentation

Isolation and identification of new bacterial stains producing equol from Pueraria lobata extract fermentation

Cleavage of Methyl Ethers by O-Desmethylangolensin-Producing Clostridium Strain SY8519

Characterization of an O-desmethylangolensin-producing bacterium isolated from human feces

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