Rapid and complete adsorption of unconjugated protein from protein-polysaccharide conjugate vaccines

Shafer, Douglas E; Schuman, Richard F.; Lees, Andrew

doi:10.1016/s0264-410x(00)00383-2

Cited by 9 publications

(2 citation statements)

References 13 publications

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“…For vaccinotherapy, polysaccharide−protein conjugates were usually prepared by covalently conjugating polysaccharides to carrier proteins. The conjugation of polysaccharide to carrier proteins generally enhances polysaccharide immunogenicity and renders the immune response T-cell dependent . In the field of tissue repairing, various methods of preparing protein- and polysaccharide-comprised materials have been developed for extracellular design.…”

Section: Introductionmentioning

confidence: 99%

“…The conjugation of polysaccharide to carrier proteins generally enhances polysaccharide immunogenicity and renders the immune response T-cell dependent. 1 In the field of tissue repairing, various methods of preparing protein-and polysaccharide-comprised materials have been developed for extracellular design. Polypeptide growth factors showed high affinity to sulfate polysaccharide (glycosaminoglycans such as heparin) and played an important role for angiogenesis, osteogenesis, chondrogenesis, and granulation tissue formation.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

2005

Biomacromolecules

153

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Polysaccharide-protein conjugations have generated increasing interests for biomedical applications in recent years. A naturally occurring cross-linking reagent, genipin, which has been used in herbal medicine, was employed to cross-link chitosan and gelatin for the preparation of a novel chitosan-gelatin conjugate. The primary amine groups on chitosan and gelatin were covalently linked with genipin, leading to the formation of a chitosan-gelatin conjugate with nitrogen-containing heterocycle units, the pyrindine-like derivatives. The FT-IR and UV-vis studies revealed that chitosan could react with genipin via a nucleophilic ring-opening reaction to construct more sufficient and extensive cross-link networks, as compared with its gelatin counterpart. The UV-vis absorption properties of the chitosan-gelatin conjugates were strongly related to the chitosan-to-gelatin weight ratio in the compositions. It is worth noting that the conjugation process endows the special emission properties of the chitosan-gelatin conjugates, which depends on the cross-linking reaction and the formation of hydrogen bonding involved chitosan-gelatin complex. Fluorescence quenching or enhancement was observed from the chitosan-gelatin conjugates upon coordinated with a wide variety of heavy metal ions (Ag+, Cu2+, Fe2+, and Co2+). This study also examined the possibility of covalent coupling the capture chelator (chitosan) with bioactive protein (e.g., albumin, alpha-globulin, and fibrinogen) to create fluorescence emission. These findings may provide a novel way to deliver therapeutic radionuclides for immuno-targeting purposes in the future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

2005

Biomacromolecules

153

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Fractionation and characterization of a protein–polysaccharide complex from Pleurotus tuberregium sclerotia

Tao¹,

Fan²,

Zhang³

2007

J Polym Sci B Polym Phys

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A water‐soluble sample (TM4b), extracted from sclerotia of Pleurotus tuberregium, was analyzed using elemental analysis, one‐ and two‐dimensional 1H and 13C NMR. The results indicated that TM4b was protein–polysaccharide complex, and the polysaccharide moiety was hyperbranched β‐D‐glucan with residuals branched at C3, C2, C4, and C6 positions. A preparative size‐exclusion chromatography (SEC) column combined with nonsolvent addition method was used to fractionate TM4b, and nine fractions were obtained. Solution properties of TM4b in 0.15 M aqueous NaCl were studied using static laser light scattering and viscometry at 25 °C. The dependences of intrinsic viscosity ([η]) and radius of gyration (〈S2〉) on weight–average molecular weight (Mw) for TM4b in the Mw range from 1.89 × 104 to 2.58 × 106 were found to be [η] = 0.21M and 〈S2〉 = 3.63M. It indicated that TM4b existed as compact sphere conformation in the aqueous solution. Atomic force microscopy image further confirmed that the TM4b molecules exhibited globular shape in the solution. This work gave valuable information on fractionation and chain conformation characterization of the globular protein–polysaccharide complex. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 45: 2546–2554, 2007

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Binding to G-quadruplex RNA activates the mitochondrial GTPase NOA1

Al-Furoukh

Goffart

Szibor

et al. 2013

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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NOA1 is an evolutionary conserved, nuclear encoded GTPase essential for mitochondrial function and cellular survival. The function of NOA1 for assembly of mitochondrial ribosomes and regulation of OXPHOS activity depends on its GTPase activity, but so far no ligands have been identified that regulate the GTPase activity of NOA1. To identify nucleic acids that bind to the RNA-binding domain of NOA1 we employed SELEX (Systemic Evolution of Ligands by EXponential Enrichment) using recombinant mouse wildtype NOA1 and the GTPase mutant NOA1-K353R. We found that NOA1 binds specifically to oligonucleotides that fold into guanine tetrads (G-quadruplexes). Binding of G-quadruplex oligonucleotides stimulated the GTPase activity of NOA1 suggesting a regulatory link between G-quadruplex containing RNAs, NOA1 function and assembly of mitochondrial ribosomes.

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Rapid and complete adsorption of unconjugated protein from protein-polysaccharide conjugate vaccines

Cited by 9 publications

References 13 publications

Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

Synthesis and Characterization of a Novel Chitosan−Gelatin Bioconjugate with Fluorescence Emission

Fractionation and characterization of a protein–polysaccharide complex from Pleurotus tuberregium sclerotia

Binding to G-quadruplex RNA activates the mitochondrial GTPase NOA1

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