Fractionation and characterization of a protein–polysaccharide complex from Pleurotus tuberregium sclerotia

Abstract

A water‐soluble sample (TM4b), extracted from sclerotia of Pleurotus tuberregium, was analyzed using elemental analysis, one‐ and two‐dimensional 1H and 13C NMR. The results indicated that TM4b was protein–polysaccharide complex, and the polysaccharide moiety was hyperbranched β‐D‐glucan with residuals branched at C3, C2, C4, and C6 positions. A preparative size‐exclusion chromatography (SEC) column combined with nonsolvent addition method was used to fractionate TM4b, and nine fractions were obtained. Solution properties of TM4b in 0.15 M aqueous NaCl were studied using static laser light scattering and viscometry at 25 °C. The dependences of intrinsic viscosity ([η]) and radius of gyration (〈S2〉) on weight–average molecular weight (Mw) for TM4b in the Mw range from 1.89 × 104 to 2.58 × 106 were found to be [η] = 0.21M and 〈S2〉 = 3.63M. It indicated that TM4b existed as compact sphere conformation in the aqueous solution. Atomic force microscopy image further confirmed that the TM4b molecules exhibited globular shape in the solution. This work gave valuable information on fractionation and chain conformation characterization of the globular protein–polysaccharide complex. © 2007 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 45: 2546–2554, 2007

Chain conformation and bioactivity of water‐soluble polysaccharide extracted from Rhizoma Panacis Japonici

“…The A value for RPS3 was calculated by Eq. (7) from the slope to be 1.48 ± 0.05 Å, which is the same as that 1.48 Å of a hyperbranched glycoprotein extracted from sclerotia of Pleurotus tuber‐regium 30…”

pH Dependence of Structure and Surface Properties of Microbial EPS

“…In static LLS, the angular dependence of the excess Rayleigh ratio ( R ex ) was measured. the R ex is related to the weight-averaged molecular weight ( M w ), the z -average root-mean-square radius of gyration ⟨ R g 2 ⟩ z 1/2 (or simply as ⟨ R g ⟩) as follows: , K C R normale normalx ≈ 1 M normalw ( 1 + 1 3 ⟨ R g 2 ⟩ z q 2 ) + 2 A 2 C where K = 4π 2 n 2 (d n /d C ) 2 /( N A λ 0 4 ) with n , dn / dC , N A , and λ 0 being the solvent refractive index, the specific refractive index increment, Avogadro’s number, and the wavelength of light in vacuum, respectively. The q = (4 πn /λ 0 )­sin­(θ/2) with θ being the scattering angle.…”

“…In static LLS, the angular dependence of the excess Rayleigh ratio (R ex ) was measured. the R ex is related to the weightaveraged molecular weight (M w ), the z-average root-meansquare radius of gyration ⟨R g 2 ⟩ z 1/2 (or simply as ⟨R g ⟩) as follows: 12,13…”

Surface decoration of selenium nanoparticles by mushroom polysaccharides–protein complexes to achieve enhanced cellular uptake and antiproliferative activity

“…With an increasing number of Se atoms, they aggregated to form SeNPs. PSP molecules, which are globular structures with a large number of hydroxyl and imino groups, 39 reacted with the surface of the SeNPs to form good dispersible and stable nanoparticles (PSP-SeNPs). Without PSP surface decoration, SeNPs were seriously aggregated and precipitated, while the PSP-SeNPs existed as monodispersed spherical particles in aqueous solution with an average diameter <50 nm (Fig.…”