Rapid analysis of docetaxel in human plasma by tandem mass spectrometry with on-line sample extraction

Development and validation of a nylon6 nanofibers mat-based SPE coupled with HPLC method for the determination of docetaxel in rabbit plasma and its application to the relative bioavailability study

“…In the literature, DTX was extracted from rat or human plasma using LLE technique with ethyl acetate [13], methyl tertiary butyl ether [12,14,15,17], acetonitrile/n-butyl chloride [16]. The volume of plasma was 0.05-1 mL and the organic solvent consumption was 0.6-4.05 mL.C18 [18,19,23], CBA-bonded silica beads [20], CN [21,24] or HLB [22] SPE columns have been used for DTX extracted from plasma samples, and the mass of packing material was at the range of 50-200 mg.…”

“…Based on the literature review, HPLC method has been used for the quantitative determination of DTX in plasma and methods for sample pretreatment mainly included liquid-liquid extraction (LLE) [8,[12][13][14][15][16][17] and solid-phase extraction (SPE) [18][19][20][21][22][23][24]. SPE is today the most popular sample preparation method due to its low consumption of organic solvents, simplicity, high recovery, highpreconcentration factors, easily to be automated and operated.…”

Determination of endocannabinoid receptor antagonist SR141716 (rimonabant) in plasma by liquid chromatograph tandem mass spectrometry

“…Although CH 3 OH could dissolve the compounds, its high methanol content prevented its use in preparation of plasma calibrators and controls. As previously reported, as little as 1% of methanol could cause protein precipitation and resulted in poor reproducibility in analytical measurement [18]. Therefore, a suitable solvent that does not precipitate plasma protein was sought for SR141716 and AM251 standard solutions.…”

A Rapid and Sensitive UPLC–MS/MS Method for Determination of Docetaxel in Rabbit Plasma: Pharmacokinetic Study of New Lung-Targeting Docetaxel Liposome at Low Dose

“…One potential problem with the use of lower DTX dose is the difficulty in determining drug levels in plasma over a time frame that is necessary for the accurate assay of pharmacokinetic parameters. To date, the general analytic method for DTX in biological matrices is HPLC [12,[14][15][16][17] and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) [18][19][20][21][22][23][24][25]. The lower limit of quantitation (LLOQ) of HPLC with UV detection assays for DTX analysis has been reported to be 2.5-12.5 ng/ml in plasma [12,[14][15][16][17].…”

“…So LC-MS/MS method with more specificity and lower LLOQ is needed. Although the LLOQ of LC-MS/MS can be 0.15-1 ng/mL, the complicated and time-consuming plasma sample pretreatment such as liquid-liquid extractions (LLE) [18][19][20], solid phases extractions (SPE) [21,22], on-line extraction [23], and column switching [24] is also required to purify analyte from the interfering components of the plasma matrix. Therefore, in the current research, a simple, sensitive, and more rapid LC-MS/MS using a one-step protein precipitant pretreatment and optimized chromatography condition is developed and validated for analysis of DTX in rabbit plasma.…”