Rapid Accumulation of Phosphatidylinositol 4,5-Bisphosphate and Inositol 1,4,5-Trisphosphate Correlates with Calcium Mobilization in Salt-Stressed Arabidopsis

DeWald, Daryll B.; Torabinejad, Javad; Jones, C. E.; Shope, Joseph C.; Cangelosi, Amanda R.; Thompson, James E.; Prestwich, Glenn D.; Hama, Hiroko

doi:10.1104/pp.126.2.759

Cited by 271 publications

(273 citation statements)

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“…Inositol 1,4,5-triphosphate (IP3) acts as a secondary messenger in signal transduction pathways involving cytosolic Ca 2+ elevations in plants (29). In these signaling pathways, cytosolic IP3 generation on perception of an external stimulus leads to cytosolic Ca 2+ elevation because of activation of IP3-sensitive tonoplast-localized Ca 2+ channels (30); the resulting cytosolic Ca 2+ spike occurs because of release of intracellular Ca 2+ stores.…”

Section: His-gchismentioning

confidence: 99%

Ca ² ⁺ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca ²⁺ channels

Verma

Gehring

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

238

240

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A family of peptide signaling molecules (AtPeps) and their plasma membrane receptor AtPepR1 are known to act in pathogendefense signaling cascades in plants. Little is currently known about the molecular mechanisms that link these signaling peptides and their receptor, a leucine-rich repeat receptor-like kinase, to downstream pathogen-defense responses. We identify some cellular activities of these molecules that provide the context for a model for their action in signaling cascades. AtPeps activate plasma membrane inwardly conducting Ca 2+ permeable channels in mesophyll cells, resulting in cytosolic Ca 2+ elevation. This activity is dependent on their receptor as well as a cyclic nucleotide-gated channel (CNGC2). We also show that the leucine-rich repeat receptor-like kinase receptor AtPepR1 has guanylyl cyclase activity, generating cGMP from GTP, and that cGMP can activate CNGC2-dependent cytosolic Ca 2+ elevation. AtPep-dependent expression of pathogen-defense genes (PDF1.2, MPK3, and WRKY33) is mediated by the Ca 2+ signaling pathway associated with AtPep peptides and their receptor. The work presented here indicates that extracellular AtPeps, which can act as danger-associated molecular patterns, signal by interaction with their receptor, AtPepR1, a plasma membrane protein that can generate cGMP. Downstream from AtPep and AtPepR1 in a signaling cascade, the cGMP-activated channel CNGC2 is involved in AtPep-and AtPepR1-dependent inward Ca 2+ conductance and resulting cytosolic Ca 2+ elevation. The signaling cascade initiated by AtPeps leads to expression of pathogen-defense genes in a Ca 2+ -dependent manner.calcium signaling | plant innate immunity | flagellin receptor 2 | brassinosteroid associated kinase 1

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Section: His-gchismentioning

confidence: 99%

Ca ² ⁺ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca ²⁺ channels

Verma

Gehring

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

238

240

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“…Excess Na + -induced membrane depolarization may activate mechanosensitive Ca 2+ channels to generate Ca 2+ signature under salt stress (4,17). Pharmacological studies and genetic analysis have shown the involvement of inositol 1,4,5-tris phosphate (IP 3 )-gated Ca 2+ channels in the regulation of Ca 2+ signature during salt stress (24)(25)(26). The FRY1 locus of Arabidopsis encodes an inositol polyphosphate 1-phosphatase, which catabolizes IP 3 .…”

Section: Second Messengersmentioning

confidence: 99%

Salt Stress Signaling and Mechanisms of Plant Salt Tolerance

Chinnusamy

Zhu

Genetic Engineering: Principles and Methods

230

151

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INTRODUCTIONSoil salinity of agricultural land has led to the breakdown of ancient civilizations.Even today it threatens agricultural productivity in 77 mha of agricultural land, of which 45 mha (20% of irrigated area) is irrigated and 32 mha (2.1% of dry land) is unirrigated (1). Salinization is further spreading in irrigated land because of improper management of irrigation and drainage. Rain, cyclones and wind also add NaCl into the coastal agricultural land. Soil salinity often leads to the development of other problems in soils such as soil sodicity and alkalinity. Soil sodicity is the result of the binding of Na + to the negatively charged clay particles, which leads to clay swelling and dispersal. Hydrolysis of the Na-clay complex results in soil alkalinity. Thus, soil salinity is a major factor limiting sustainable agriculture.The USDA salinity laboratory defines saline soil as having electrical conductivity of the saturated paste extract (EC e ) of 4 dS m -1 (1 dS m -1 is approximately equal to 10 mM NaCl) or more. High concentrations of soluble salts such as chlorides of sodium, calcium and magnesium contribute to the high electrical conductivity of saline soils.NaCl contributes to most of the soluble salts in saline soil.The development of salinity-tolerant crops is the need of the hour to sustain agricultural production. Conventional breeding programs aimed at improving crop tolerance to salinity have limited success because of the complexity of the trait (2). Slow progress in breeding for salt-tolerant crops can be attributed to the poor understanding of the molecular mechanisms of salt tolerance. Understanding the molecular basis of plant salt tolerance will also help improve drought and extreme-temperature-stress tolerance, since osmotic and oxidative stresses are common to these abiotic stresses. The salt-2 tolerant mechanisms of plants can be broadly described as ion homeostasis, osmotic homeostasis, stress damage control and repair, and growth regulation (3). This chapter reviews recent progress in understanding salt-stress signaling and breeding/genetic engineering for salt -tolerant crops. EFFECT OF SALINITY ON PLANT DEVELOPMENTSalinity affects almost all aspects of plant development, including germination, vegetative growth and reproductive development. Soil salinity imposes ion toxicity, osmotic stress, nutrient (N, Ca, K, P, Fe, Zn) deficiency and oxidative stress on plants.Salinity also indirectly limits plant productivity through its adverse effects on the growth of beneficial and symbiotic microbes. High salt concentrations in soil impose osmotic stress and thus limit water uptake from soil. Sodium accumulation in cell walls can rapidly lead to osmotic stress and cell death (1). Ion toxicity is the result of replacement of K + by Na + in biochemical reactions, and Na + and Cl --induced conformational changes in proteins. For several enzymes, K + acts as cofactor and cannot be substituted by Na + .High K + concentration is also required for binding tRNA to ribosomes and thus protein synt...

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“…12 Important roles of PLC in plant cell responses to hyperosmotic stress and drought in different plant systems are well documented. 13,14 The PLC/DAGK-derived PA also seems to be the prominent species involved in biotic stress tolerance. Thus, tomato cell suspensions responded to a number of pathogenic elicitors by increasing their PA contents within minutes of elicitation, which arose mostly via DAG phosphorylation and was correlated with decreased levels of PtdIns(4,5)P 2 .…”

Section: Introductionmentioning

confidence: 99%

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

et al. 2014

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Phosphatidylinositol-specific phospholipase C2 (PLC2) is a signaling enzyme with hydrolytic activity against membrane-bound phosphoinositides. It catalyzes the cleavage of phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5) P 2 ) into two initial second messengers, myo-inositol-1,4,5-trisphosphate (InsP 3 ) and diacylglycerol (DAG). The former, as well as its fully phosphorylated derivative, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP 6 ), play a major role in calcium signaling events within the cell, while DAG may be used in the regeneration of phospholipids or as a precursor for phosphatidic acid (PA) biosynthesis, an important signaling molecule involved in both biotic and abiotic types of stress tolerance. Overexpression of the gene for Brassica napus phospholipase C2 (BnPLC2) in Brassica napus has been shown to enhance drought tolerance, modulate multiple genes involved in different processes and favorably affect hormonal levels in different tissues. We, therefore, undertook the current study with a view to examining, at the metabolome level, its effect on both abiotic (low temperature) and biotic (stem white rot disease) types of stress in canola. Thus, while transgenic plants exhibited a significant rise in maltose levels and a concomitant elevation in some unsaturated free fatty acids (FFAs), glycerol, and glycerol 3-phosphate under subzero temperatures, they accumulated high levels of raffinose, stachyose and other sugars as well as some flavonoids under acclimatization conditions. Collectively, overexpression of BnPLC2 appears to have triggered different metabolite patterns consistent with its abiotic and, to a limited extent, biotic stress tolerance phenotypes.

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Rapid Accumulation of Phosphatidylinositol 4,5-Bisphosphate and Inositol 1,4,5-Trisphosphate Correlates with Calcium Mobilization in Salt-Stressed Arabidopsis

Cited by 271 publications

References 54 publications

Ca ² ⁺ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca ²⁺ channels

Ca ² ⁺ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca ²⁺ channels

Salt Stress Signaling and Mechanisms of Plant Salt Tolerance

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

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Rapid Accumulation of Phosphatidylinositol 4,5-Bisphosphate and Inositol 1,4,5-Trisphosphate Correlates with Calcium Mobilization in Salt-Stressed Arabidopsis

Cited by 271 publications

References 54 publications

Ca 2 + signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca 2+ channels

Ca 2 + signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca 2+ channels

Salt Stress Signaling and Mechanisms of Plant Salt Tolerance

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

Contact Info

Product

Resources

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Ca ² ⁺ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca ²⁺ channels

Ca ² ⁺ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca ²⁺ channels