Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair

Tao, Jie; Zhang, Jiumeng; Du, Ting; Xu, Xin; Deng, Xianming; Chen, Shaochen; Liu, Jinlu; Chen, Yu‐Wen; Li, Xuan; Xiong, Meimei; Luo, Yi; Cheng, Hao; Mao, Jian; Cardon, Ludwig; Gou, Maling; Wei, Yuquan

doi:10.1016/j.actbio.2019.03.047

Cited by 121 publications

(93 citation statements)

References 54 publications

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“…Vijayavenkataraman et al (2019) fabricated 3D porous NGCs using a biodegradable and conductive block copolymer of PPy and a novel electrohydrodynamic jet 3D printing process, which can support higher growth of neural cells and a stronger maturation of hESC-NCSCs to peripheral neuronal cells. Tao et al (2019) manufactured a functional nanoparticle-enhanced nerve conduit for promoting the regeneration of peripheral nerves, which consists of gelatin-methacryloyl (GelMA) hydrogels with drug-loaded poly (ethylene glycol)-poly (3-caprolactone; MPEG-PCL) nanoparticles dispersed in the hydrogel matrix and rapidly fabricated by a continuous 3D printing. It is unknown whether a better result would be obtained by bridging the nerve gap with a more suitable ANG and tissue engineered nerve grafts in which the BM is more similar in components and thickness to the injured nerve; therefore, related animal studies are ongoing.…”

Section: Discussionmentioning

confidence: 99%

Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats

Wei

Cheng

et al. 2020

Front. Neuroanat.

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Section: Discussionmentioning

confidence: 99%

Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats

Wei

Cheng

et al. 2020

Front. Neuroanat.

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“…Gelatin combined with other hydrogels has been used to optimize printability, improving shape retention during printing. For example, gelatin-alginate [240,[319][320][321], Laponite [322], nanoclays [323], and synthetic polymers such as Pluronic F-127 [208] and PCL [324], have all been studied. Alginate dialdehyde-gelatin scaffolds were printed in the presence of a cross-linker for reaching feature sizes of~500 µm [245], while alginate-GelMA interpenetrating networks via UV crosslinking of GelMA followed by Ca crosslinking of alginate was reported [321].…”

Section: Structural and Mechanical Propertiesmentioning

confidence: 99%

“…Inclusion of alginate [240,[319][320][321], Laponite [322], nanoclays [323], Pluronic F-127 [208], PCL [324]; Feature size 500 µm [245] [ [313][314][315][316]; Feature size 300 µm (SLA), Compressive E: 0.5-18 MPa [328] Mouse planta dermis [319]; dental pulp stem cells [320]; hMSCs and amniotic epithelial cells [240]; chondrocytes [214,327] Silk ( Mouse articular chondrocytes [342]; human fibroblasts [338]; porcine chondrocytes [340]; hMSCs [344,345]; human mesenchymal progenitor cells [346]…”

Section: ) Applicationsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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“…3D bioprinting technology has been proved to be a promising tool for fabricating nerve conduits in a customized and precise manner. [19,20] It allows the integration of biomaterials and biofactors into the conduits in a convenient and efficient process. Here, we introduced the integration of platelets and a hydrogel nerve conduit through a continuous 3D bioprinting process for promoting peripheral nerve regeneration (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

3D‐Printed Nerve Conduits with Live Platelets for Effective Peripheral Nerve Repair

Tao

Liu

et al. 2020

Adv Funct Materials

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Platelets can secrete multiple growth factors for peripheral nerve repair. However, topically injected platelets are rapidly activated followed by burst release of the cargos, causing limited therapeutic efficiency in vivo. Herein, a platelet-incorporated hydrogel conduit is shown that acts as a biosynthetic nerve conduit for peripheral nerve repair. This conduit is prepared by rapid 3D printing of the live platelets mixed with bio-ink containing gelatin methacrylate (GelMA) and poly(ethyleneglycol)diacrylate (PEGDA). The hydrogel can significantly prolong the survival of the incorporated platelets. While the conduit provides a physical foundation for bridging the nerve gaps, live platelets in the conduit sustained-release multiple growth factors to promote the nerve repair. The in vivo therapeutic efficiency of this platelet-incorporated nerve conduit is tested in bridging a 10 mm gap in sciatic nerves. The results of morphological, electrophysiological, and histological assessments indicate that the incorporated platelets can significantly promote the hydrogel conduits in peripheral nerve repair. The demonstrated 3D bioprinted nerve conduit with live platelets may show potential clinical application in peripheral nerve repair.

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Rapid 3D printing of functional nanoparticle-enhanced conduits for effective nerve repair

Cited by 121 publications

References 54 publications

Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats

Differences in the Structure and Protein Expression of Femoral Nerve Branches in Rats

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

3D‐Printed Nerve Conduits with Live Platelets for Effective Peripheral Nerve Repair

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