RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.

Abstract

The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed.

Regeneration and assessment of genetic fidelity of the endangered tree Moringa peregrina (Forsk.) Fiori using Inter Simple Sequence Repeat (ISSR)

“…Martins et al (2004) reported the genetic stability of micropropagated almond plants. The genetic stability of in vitro propagated Picea mariana was also reported by Isabel et al (1993) and in vitro-adventitious shoots of Pinus taeda (Tang 2001) and chestnut (Carvalho et al 2004). PCR based molecular markers including ISSR are very simple, fast, reliable, highly discriminative and inexpensive.…”

Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)

“…Some genetic molecular markers, such as RFLP (DeVerno et al 1994), RAPD (Isabel et al 1993;Fourré et al 1997) and AFLP (Hornero et al 2001), have been used for analysis of true-to-typeness. Microsatellites can serve as highly sensitive markers for monitoring genetic variation that may signal potential deleterious mutations during in vitro culture, because they reflect a relatively high rate of mutation and corresponding degree of genetic variability (Burg et al 1999;Leroy et al 2000;Wilhelm et al 2005;Lopes et al 2006;Burg et al 2007).…”

Karyotype analysis and optimization of mitotic index in Picea mariana (black spruce) preparations from seedling root tips and embryogenic cultures

“…The arm ratios and relative chromosome values did not show variation between embryogenic cultures and root tips or among the different lines studied. Recent molecular studies on genetic stability of black spruce embryogenic cultures showed no variation within the clones (Isabel et al, 1993). The basis for the apparent genetic stability of these embryogenic cultures is unknown but may be related to their embryogenic character (Eastman et a!., 1991 In herbaceous plants, however, the occurrence of…”