RAPD polymorphism of wild emmer wheat populations, Triticum dicoccoides, in Israel

Fahima, Tzion; Sun, Genlou; Beharav, Alex; Krugman, Tamar; Beiles, Avigdor; Nevo, Eviatar

doi:10.1007/s001220051089

Cited by 128 publications

(100 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The Proc Stepdisc procedure of the SAS software was used (SAS Institute, 1996, Fahima et al, 1999. This procedure was applied to the EST-SSR and gSSR markers, separately.…”

Section: Discriminant Analysismentioning

confidence: 99%

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Missio

Caixeta²,

Zambolim³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

“…The Proc Stepdisc procedure of the SAS software was used (SAS Institute, 1996, Fahima et al, 1999. This procedure was applied to the EST-SSR and gSSR markers, separately.…”

Section: Discriminant Analysismentioning

confidence: 99%

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Missio

Caixeta²,

Zambolim³

et al. 2011

Genet. Mol. Res.

View full text Add to dashboard Cite

“…Random amplified polymorphic DNA (RAPD) analysis has been successfully used to detect genetic variation of plant populations in a large number of studies (Fahima et al, 1999;Mengistu et al, 2000;Persson and Gustavsson, 2001;Arafeh et al, 2002) and to analyse genetic differentiation among populations from ecologically different habitats (Owuor et al, 1997;Huff et al, 1998;Kö lliker et al, 1998;Hsao and Lee, 1999). Moreover, the analysis of molecular variance technique (AMOVA) (Excoffier et al, 1992) is a powerful tool to analyse RAPD marker variation within and among plant populations (Huff et al, 1993;Huff, 1997).…”

Section: Introductionmentioning

confidence: 99%

Genetic differentiation among populations of Sesleria albicans Kit. ex Schultes (Poaceae) from ecologically different habitats in central Europe

2003

View full text Add to dashboard Cite

As observed for many other plant species, the populations of Sesleria albicans in Central Europe are located in habitats, which differ to a high degree from each other with regard to ecological factors such as nutrients, light and water as well as in type of land use. The species colonizes limestone cliffs, pavements, screes, grazed and mown grasslands, heaths, fens and open woodlands. In this study, we used random amplified polymorphic DNA (RAPD) analysis to investigate the genetic differentiation among 25 populations of S. albicans from six different types of habitat (beech forests, alpine and lowland rocky ridges, lowland screes, fens, calcareous grasslands). With RAPD analysis, 344 fragments could be amplified, of which 95.9% were polymorphic. The level of polymorphism ranged from 29.7 to 56.7% polymorphic bands per population and was correlated with population size. In an analysis of molecular variance (AMOVA), used to detect variation among individuals within populations, among populations from the same habitat and among different habitats, most of the genetic variation was found within populations (62.06%) and among populations from the same habitat (33.36%). In contrast, only a very low level of differentiation could be observed among different habitats (4.58%). The results of our study give only little evidence for an ecotypic differentiation of Sesleria albicans. This differentiation is principally conceivable, but obviously not related to the investigated RAPD loci.

show abstract

“…Despite the rather small territory of Lithuania, the ecological-climatological conditions in the eastwest and south-north direction are rather different [44], and climate warming may be the one of reasons for the spread of E. annuus in the northern direction. Small but significant differences (8% for RAPD markers; 5% for ISSR markers) between molecular variance found among the three regions considered in our work ( Table 5) and high population genetic structuring (Φ PR =0.49 for RAPD markers; Φ PR =0.58 for ISSR markers) can be explained in several ways, including the multiple founders and possible habitat-genotype interaction [30,45,46]. Trtikova et al [17] also identified a high level of genetic differentiation among populations from Switzerland.…”

Section: Discussionmentioning

confidence: 59%

Clonal structure and reduced diversity of the invasive alien plant Erigeron annuus in Lithuania

et al. 2013

View full text Add to dashboard Cite

The alien species Erigeron annuus (L.) Pers. is in an intensive spreading phase in Lithuania. Random amplified polymorphic DNA (RAPDs) and inter-simple sequence repeats (ISSRs) assays were used to study the genetic structure of old and new invasive populations and to determine the most spread genotypes of this species in Lithuania. Pairwise genetic distances between populations established using RAPD and ISSR markers significantly correlated (r=0.91, P<0.05). Our study indicates that there are two genetically different types of E. annuus populations. The first type is represented by a widely spread main clone and related monomorphic populations. The second type is represented by polymorphic populations, some of them present at sites where E. annuus has not been previously observed. Main clone predominates in nine populations and is from the region where this species was first described in natural ecosystems of Lithuania. UPGMA cluster analysis revealed genetic relationships between the main clone and accessions from old cemeteries where E. annuus has been grown as an ornamental plant. We found high genetic differentiation among populations (G ST=0.58 for RAPDs, G ST=0.64 for ISSRs). Taken together, our results will contribute to the monitoring of E. annuus spread in Lithuania.

show abstract

RAPD polymorphism of wild emmer wheat populations, Triticum dicoccoides, in Israel

Cited by 128 publications

References 29 publications

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Genetic differentiation among populations of Sesleria albicans Kit. ex Schultes (Poaceae) from ecologically different habitats in central Europe

Clonal structure and reduced diversity of the invasive alien plant Erigeron annuus in Lithuania

Contact Info

Product

Resources

About